subculturing 中文意思是什麼

subculturing 解釋
傳代培養
  1. There are also different in inducing cluster buds from callus of clone gingkgo and its other tissues and organs, some callus can induce cluster buds, but others can not. differentiation rate from the callus of cotyledon can be up to 36 % and the number of cluster buds is positive related to the times of subculturing

    但是不同器官誘導的愈傷組織對于叢生芽的誘導也是不同的，子葉誘導的愈傷組織的分化率較高，有36左右，並且繼代次數的增加也能夠增加分化的幾率
  2. Many researchers have conducted experimellts about it, but not succeeded. culture media were compared to find the best medium of gingkgo culture for controlling callus browning by different sugars, antioxidants and sorbents. the result showed that the medium with ms + zt 1. 0mg / l + naa 1. 0mg / l + sucrose 50g / l + agar 8g / l + ac 2g / l was the best medium, at l5 days subculturing intervals

    銀杏組織培養過程中，尤其是在愈傷組織的繼代培養中，褐變現象特別嚴重，曾有不少的人做過這方面的研究，但都沒有成功，而本研究通過對不同糖類物質、抗氧化劑、吸附劑以及不同的培養基對褐變的影響和控制效果，探索出有效控制褐化現象發生的最佳培養條件，試驗結果表明： ms + zt1 . 0mg / l + naa1 . 0mg / l +蔗糖50g / l +瓊脂8g / l + ac2g / l培養基上的繼代效果最好，繼代時間最好在15d左右。
  3. Twenty one adventitious buds from 30 root segment cultures were obtained after subculturing on medium lacking growth regulator for 90 days. the effects of hormones 6 - ba and naa on the adventitious bud differentiation were investigated and optimized. the results were showed that 6 - ba had significant effect on bud differentiation and the optimized medium for adventitious bud differentiation was ms + l. 0mg / l naa + 0. 5mg / l 6 - ba. there were 67 adventitious buds occurred from average 30 hairy root segment cultures

    90d后統計不定芽數量，平均每30個根段培養物上可獲得21個不定芽；添加0 . 5mg 16 - ba能顯著地促進毛狀根不定芽的分化，配合使用6 - ba和naa對毛狀根不定芽的分化最為有效（ ms + 1 . 0mg lnaa + 0 . 5mg l6 - ba ） ， 90d后統計，平均每30個根段培養物上可獲得67個不定芽。
  4. The apoptosis in the passage cells was detected by using flow cytometry. it was fmded that the apoptosis of passage 12 cells was not obvious. cells were subcultured to sixteenth passage. it proved that the subculturing of porcine ear skin fibroblasts was stable by using this kind of method combining trypsin cold treatment with trypsin heat treatment

    在進行傳代培養時，利用了流式細胞儀檢測了培養細胞的凋亡情況，發現傳至第12代的細胞仍無明顯凋亡現象，細胞已傳至16代以上，說明應用此方法可穩定地進行豬耳皮膚成纖維細胞的傳代培養。
  5. Effects of cell density on proliferation and osteogenic differentiation of human mesenchymal stem cells during subculturing in vitro

    傳代培養密度對人間充質幹細胞增殖及成骨分化的影響
  6. The highest yield of protoplasts ( 4. 2 x 106 / g f. wt. ) was obtained from 12 - day - old calli after subculturing on fresh medium

    結果表明，用轉代後生長12d的愈傷組織，分離的原生質體產率為4
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