substrate protein 中文意思是什麼

substrate protein 解釋
底物蛋白
  • substrate : n. 1. 底層，地層。2. 【無線電】（半導體工藝中的）襯底，基底。3. 【生物學】（生態學中的）基層；【生物化學】受質；被酶作用物。
  • protein : n. 【化學】朊，蛋白（質）。
  1. According to the research of physiological and biochemical indicators or index, components of soluble proteins, substrate protein of phosphorylation and the activity of protein kinase in low - temperature stress in the leaves of brassica oleracea l., we tried to find the law of the physiological and biochemical response of brassica oleracea l. leaf to low temperature. at the same time, discussion on the signal transduction can also provide further evidences for revealing the mechanism of low - temperature stress. the results are showed as follows : malondialdehyde ( mda ), superoxide dismutase ( sod ), ascorbate peroxidase ( asp ) and peroxidase ( pod ) activities were changed greatly after 0 ~ 30min ' s treating with low temperature

    本文以甘藍葉片為材料，通過對低溫5脅迫下甘藍生理生化指標、可溶性蛋白組分以及磷酸化底物蛋白、蛋白激酶活性的研究，以期找出甘藍葉片對低溫脅迫的生理生化響應規律，為甘藍露地越冬栽培防範寒害提供理論指導，同時對低溫脅迫下甘藍逆境信號傳導進行了探討，從而為徹底弄清低溫脅迫機理提供進一步的證據，研究的主要結果如下：丙二醛含量（ mda ） 、超氧化物歧化酶（ sod ） 、抗壞血酸過氧化物酶（ asp ）和過氧化物酶（ pod ）活性在低溫處理0 30min發生顯著變化，低溫處理3min后，甘藍葉片內mda含量基本沒有變化，處理5min時出現第一個峰值，達到對照的104 . 10 ， 10min出現低谷，僅為對照的86 . 27 ，隨后再次上升， 30min時超過第一峰值，為對照的113 . 93 。
  2. Vanadate, a potent ptpase inhibitor, locks adipocyte differentiation at an early stage in the program and leds to the accumulation of p - c - crkii, a phosphotyrosyl protein that is a substrate for ptpase ha2

    研究發現一種強烈的磷酸酯酶抑制劑- -釩酸鈉能夠作用於脂肪細胞的分化早期過程從而抑制其分化，並使一種ptpha2的底物即酪氨酸磷酸化的蛋白質p - c - crkii累積。
  3. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  4. Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases

    研究人員已經利用蛋白質工程和定點突變技術來改變各種醇腈酶的活性位點和底物特異性等。
  5. Arc1, thl1 and thl2, the substrate protein genes of s receptor kinase, were cloned through a series of methods of molecular biology such as pcr, rt - pcr, dna cloning and sequencing. the resultings sequences were highly analysed by using the related biosoftwares on internet, providing new insights in the field of the molecular mechanism of self - incompatibility in plants. the major results are as followings : 1

    本文通過pcr和rt - pcr等一系列分子生物學方法克隆了蕓薹屬植物中的甘藍和油菜自交不親和信號傳導過程中srk底物蛋白基因arc1 、 thl1和thl2 ，並使用各種相關生物信息學軟體對srk底物蛋白基因序列進行了分析，然後在internet網上利用在線軟體對蛋白質的結構和功能進行了預測和探討，以期為蕓薹屬植物自交不親和性的分子機理的研究提供新的內容。
  6. Green fluorescent protein ( gfp ) was extracted from jellyfish ( aequerea victoria ) of ocean invertebrate. gfp can emit green fluorescent when it is illuminated by light of suitable wavelength. the emissions of fluorescence need not any additional factors, such as substrate, supplementary factor

    綠色熒光蛋白（ greenfluorescentprotein ， gfp ）是來源於多管水母（ aequoreavictoria ）等海洋無脊椎動物的一種蛋白質，該蛋白質在體外經適當波長的光激發便發出綠色熒光，並且這種熒光的發射不需要任何底物和輔助因子的誘導。
  7. With the procedures, the overall recovery of enzymatic activity reached 20 % and the specific activity for substrate hydantoin was about 4 u / mg protein. the purification factor was about 4. 7 folds with the purity about more than 95 % as estimated by sds - page analysis. d - hydantoinase gene from strain ss - ori was cloned to five different vectors to be five recombinant plasmids and in turn to transfer into five different e. coli strains, respectively

    對其中產酶活性最高的一工程菌pexsec一hdt /雲coljblz ] （ de3 ）海因酶的表達條件進行了研究，目的蛋白的表達量約占總菌蛋白的20 % ，其酶活力為0 . 92u / ml ，約是原始菌株的d一海因酶表觀活性的4 . 6倍。
  8. Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells. only after adhering to surface followed by spreading can cells develop and proliferate. most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity, which may correlates to the low degree of cell attachment. modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation. cell attachment to substrate is primarily mediated by integrins, a widely expressed family of heterodimeric surface receptors. most extrcellular matrix proteins such as fibronectin, osteopontin, collagen type i, bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin. the main aim of this research is to measure, assess adhesion, proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin, collagen type i or biotie gen, which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture. ( 2 ) adhesion, proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed. ( 3 ) also, adhesion, proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin, or collagen type i respectively. ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo. this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities

    因此將這些蛋白包被、固定到材料表面，觀察骨組織工程種子細胞mscs細胞的粘附、生長特性是本研究的中心環節，並從以下方面進行探討： （ 1 ）採用不同原代細胞分離方法，研究其對mscs細胞的生物學特性影響。 （ 2 ）檢測基因勝肽膠對mscs細胞粘附、增殖及分化的影響。 （ 3 ）分別採用型膠原及纖維粘連蛋白（ fibronectin ， fn ）包被聚乙醇酸-乳酸共聚物（ poly （ 1actide - co - glycolide ） ， plga ）膜及多孔塊型plga材料，觀察細胞在單層或三維培養狀態下，型膠原及fn對mscs細胞粘附、增殖及向成骨細胞分化效應及能力。
  9. 2. proceedings of the reseaches on the novel nitrogenase mnfe protein and crfe protein several new batches of mnfe protein and crfe protein were prepared. their substrate reduction activities have been determined by the complementation with uw45 fe protein

    新型固氮酶mnfe蛋白和crfe蛋白的特性與結晶研究在已有的工作基礎上，分離純化了幾批mnfe蛋白和crfe蛋白，並用部分純的ugh fe蛋白進行活性互補，分別測定了mnfe蛋白和crfe蛋白的底物還原活性。
  10. The scfco, " ubiquitin - ligase complexes are required for jasmonate response in arabidopsis ( l ) arabidopsis coi is required for all the jasmonate - regulated response. it encodes a protein containing leucine - rich repeats and a degenerate f - box motif these structural features are characteristic of f - box proteins that function in ubiquitin ligase complexes for the ubiquitylation of substrate - proteins targeted for degradat1on

    以表達融合蛋白flag - coi1的擬南芥為材料，用- flag抗體進行免疫共沉澱分析發現， coi1蛋白可以與ask1 、 ask2 、 atrbx1和atcul1蛋白結合形成scf ~ （ coi1 ）復合體，並發現其中的atcul1蛋白包括未修飾和修飾的兩種形式。
  11. Cortactin, a novel member of filament actin binding protein family and the main substrate of non - receptor src protein kinase, plays an important role in the dynamic organization of cell cortex cytoskeleton

    皮層蛋白（ cortactin ）是一種微絲肌動蛋白結合蛋白，它與肌動蛋白纖絲的側面相結合，並直接參與皮層細胞骨架的組建。
  12. However, the ubiquitylated substrate protein of these ubcs remains unknown, and the nature of the dna to which the repair factors bind is still unclear

    這三種酶協同作用把泛素分子鏈作為標簽連接在目的蛋白上使之被26s蛋白酶體識別而降解。
  13. Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids

    Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵，包括36個氨基酸組成的疏水信號膚，一個cub （ clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl ）結構域和一個胰酶樣絲氨酸蛋白酶結構域（ t汀psin一likeserineproteasedomain ）和幾個保守的糖基化位點等，沒有發現有跨膜區的存在。
  14. Proteinmicroarrays are now becoming very popular due to their possible futureapplications in the study of nucleic acid - protein, protein - protein, ligand - receptor, drug - protein target, and enzyme - substrate interactions

    蛋白晶元正在成為相當受歡迎，由於他們的未來可能應用在研究核酸蛋白、蛋白質，配體受體藥物靶蛋白和酶基板互動
  15. Comparison of substrate - reduction properties of these altered mofe proteins with wt and nifv - mofe proteins showed that : ( 1 ) only the a - gln194 substitution did not affect the total electron to proton reduction and notably double altered mofe protein with substitution of citrate and a - lys190 only retained very poor proton reduction activity. ( 2 ) a - gln194 substitution made a more direct effect on n2 reduction then other two substitutions

    四個突變體固氮酶、野生型和nifv ~ -固氮酶的mofe蛋白的底物還原特性比較表明： （ 1 ） - gln ~ （ 194 ）替換不影響固氮酶還原質子時的總電子流；尤其引人注意的是，含有- lys ~ （ 190 ）和檸檬酸雙重替換的mofe蛋白幾乎完全失去了質子還原的能力。
  16. Haemostaseology - determination of protein c activity - reference measurement procedure with a chromogenic peptide substrate

    止血學. c蛋白活性的測定.肽基色譜參照測量程序
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