subtractive hybridization 中文意思是什麼

subtractive hybridization 解釋
減除雜交法
  1. Screening of differentially expressed genes in placentas with hepatitis b virus infection by suppression subtractive hybridization technique

    乙型肝炎病毒感染胎盤組織差異表達基因的篩選與確定
  2. In this study, sd rats were used to establish the animal model of brain injury induced by repeated + gz exposure and suppression subtractive hybridization technique was adopted to screen the differentially expressed genes in rat brains of + gz exposure group. the aim of the study was to obtain preliminary experimental data for the molecular mechanisms of the brain injury

    本研究利用大鼠重復+ 10gz暴露引發腦損傷的動物模型，觀察腦的病理學改變；應用抑制性消減雜交技術篩選+ gz重復暴露大鼠腦的差異表達基因，旨在初步探討+ gz重復暴露致腦損傷的分子機制。
  3. In virtue of the technique of suppression subtractive hybridization, we had successfully constructed a subtractive library of d. involucrata sprouting bract by taking homochronous leaf as driver in this study

    本研究利用新近發展的抑制性差減雜交技術（ ssh ）成功構建了珙桐幼嫩苞片和葉片差減文庫。
  4. In previous study, janghong have succeeded cloning 24 ests of mouse testis spermatogenic cell apoptosis - related gene by creating mouse cryptorchidism model and making use of suppression subtractive hybridization, and registered in genbank

    在前期的研究工作中，本室姜宏等通過建立小鼠隱睪模型，運用抑制消減雜交法（ suppressionsubtractivehybridization ， ssh ）克隆出24個小鼠睪丸生精細胞凋亡相關基因的est ，並在genbank中登錄。
  5. After the screening, the expression and identity of the gene for nadp - malic enzyme in leaves of aloe vera l. under salt stress had been done. methods : construction and screening of the subtractive library : to construct a cdna subtractive library of aloe vera l. under salt stress, the leaves of aloe vera l were removed from the seedlings which were treated with 300 mm nacl. suppression subtractive hybridization ( ssh ) was carried out and a subtractive library was constructed

    鹽脅迫下庫拉索蘆薈葉子中nadp -蘋果酸酶基因的表達鑒定：為了檢測蘆薈鹽脅迫下庫拉索蘆薈cdna消減又庫的構建篩選及nadp一蘋果酸酶基因的鹽誘導表達中nadp一蘋果酸酶基因『刀叻心）的表達和nadp一蘋果酸酶（ nadp一ma1ieenzyme ）蛋白的積累是否受著高鹽的誘導，我們利用耐鹽品種庫拉索蘆薈（ a了口。
  6. Part 1 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) chapter 1 construction of subtractive cdna library of mitten crab ( eriocheir japonica sinensis ) ovary two subtracted cdna libraries of mitten crab ( eriocheir japonica sinensis ) ovaries at two successive developmental stages were constructed by suppression subtractive hybridization ( ssh )

    第一部分中華絨螯蟹卵巢發育相關基因的篩選1中華絨螯蟹卵巢差減cdna文庫的構建應用抑制性差減雜交技術，構建了中華絨螯蟹卵巢兩個發育時期的差減cdna文庫。
  7. Among these techniques, the suppression subtractive hybridization ( ssh ) method has been shown to be quick and highly efficient, and it generates less false - positive clones

    將實驗方的雙鏈cdna分成兩組，分別與兩種不同的接頭連接（ adaptor1andadaptor2r ） 。
  8. Dominating methods in wheat gene cloning include homology - based cloning, map - based cloning, suppression subtractive hybridization, ddrt - pcr etc

    目前，小麥基因的克隆方法主要有同源克隆、圖位克隆、差減雜交、差異顯示等。
  9. A cdna subtractive library with high subtractive efficiency of repeated + gz exposures in rat brain was constructed with suppression subtractive hybridization ( ssh ). the cdna subtractive library after amplification included 100 blue clones and 400 white clones, 75 ones of which were selected to prepare for plasmid. identification of the clones with restriction endonuclease cleavage showed most of them had been cloned to the vector

    構建高消減效率的+ gz重復暴露大鼠腦cdna消減文庫，擴增后cdna文庫包含約400個白色克隆和100個藍色克隆，克隆飽滿清晰，隨機挑取75個白色克隆，制備質粒后，以ecor酶切分析，表明大部分克隆入質粒載體。
  10. Furthermore, suppression subtractive hybridization ( ssh ) was employed for the isolation of cdna fragments for euonymus japonicus " zhuzi " differentially expressed genes, and forward suppression subtractive cdna library of cold - regulated genes was constructed. the seedlings of euonymus japonicus " zhuzi " treated with low temperature were as tester and untreated seedlings as driver. subtractive cdna library was differentially screened through cdna macroarray, six hundreds and four cdna clones were identified as cold specifically induced or highly expressed

    （ 5 ）應用抑制差減雜交（ suppressionsubtractivehybridization ， ssh ）方法，構建冷誘導表達的正向抑制差減cdna文庫，低溫處理的幼苗為tester ，常溫處理為driver ，通過cdna微陣列差異篩選cdna文庫，得到604個低溫誘導或表達增強的候選克隆，對其中的84克隆進行dna測序，去除冗餘的cdna ，在genbank中進行核酸和蛋白質同源性的比較和功能分析，共有36個單一序列，其中12個cdna在genbank數據庫沒有同源的序列。
  11. Its virtue is to hold the relationship between phe - notype and gene. it is difficult to be replaced by other means because the gene decides the phenotype. the most classic positional cloning is " cdna - dependent ", including cdna selection, different display and subtractive hybridization

    該方法最獨特的優點是抓住了「表型」與「基因」的聯系，基因的功能是決定某一表型，因此，通過明確的表型來鑒定某一基因存在的位置與功能，是其它非遺傳技術難以取代的。
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