supf 中文意思是什麼
supf
解釋
祖普夫-
Our previous work identified delayed mutation occurred at target supf trna gene in plasmid transfected into cells pretreated with low concentration of alkylating agent n - methyl - n ' - nitro - n - nitrosoguanidine ( mnng )
在收回的質粒上我們檢測到了延遲發生的、高於對照5倍以上的突變。由於w在細胞中的半壽期僅為1 -
In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統，直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變：首先用低濃度（ 0 . 2 m ）的短壽烷化劑mnng （半壽期為1 . 1hr ）處理細胞2 . 5h后，繼續培養24h ，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。 -
Direct damage on supf trna gene can be neglected because half - life of mnng is 1. 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours. therefore the mutagenesis is not lesion directed
20m的mnng經洗滌后的極微量殘留經過10 ? 20多個半衰期的降解后，己不足攻擊轉人的qdna分子，因此這種突變顯然是發生在mnng直接攻擊部位以外的正常堿基上。
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