target enzyme 中文意思是什麼
target enzyme
解釋
靶酶-
The enzyme 5 - enolpyuvyl - 3 - phosphoshikimic acid synthase ( epsp synthase ; ec2. 5. 1. 19 ), encoded by anoa locus, is a key enzyme present in microorganisms and plants where it has a function in the biosynthesis of aromatic amino acids. glyphosate ( n - phosphonomethyl - glycine ) is an effective non - selective, broad spectrum, postemergence herbicide, which has been shown to inhibit epsp synthase activity in a competitive manner. glyphosate tolerant plants can be mediated by either overproduction of the target enzyme or by the presence of an altered enzyme
植物和微生物芳香族氨基酸生物合成過程中的一個關鍵酶? ? 5 -烯醇丙酮莽草酸- 3 -磷酸合成酶（ epspsynthase ； ec2 . 5 . 1 . 19 ）由aroa基因編碼，該酶受廣譜滅生性、內吸傳導型除草劑草甘膦的競爭性抑制，將epsp合成酶基因轉入植物中可獲得草甘膦耐受植株。 -
To target this mitochondrial enzyme into chloroplast, the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene, whereas expression of the chimeric gene was driven by the camv 35s promoter
Pchlsod質粒含有煙草mnsod基因的cdna序列，與豌豆核糖體小亞基葉綠體引物肽（ tp ）的編碼基因序列構成融合基因，由35s啟動子調控。 npt基因為選擇標記基因， pgv2260為輔助質粒。 -
They contained an antisense constructed for the spruce gene encoding ccr ( cinnamoyl alcohol dehydrogenase ), an enzyme of monolignol synthesis. the antisense rna method is a technique for reducing the expression of a resident target gene. the transgenic sublines were produced by particle bombardment at the dept of forest genetics, swedish university of agricultural sciences
本項目研究的目的就是採用瑞典農業大學構建的反義ccr基因轉導的挪威雲杉細胞愈傷組織，通過誘導產生再生植株，並檢測證實該基因是否表達及其它相關基因的表達狀況，為培育出低木質素的轉基因挪威雲杉新品種奠定物質和理論基礎。 -
The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity
首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。 -
Drug design has been relied on target enzyme which usually is a protein molecule
基於靶標結構的藥物設計方法是先導藥物設計的重要手段。 -
Proteinmicroarrays are now becoming very popular due to their possible futureapplications in the study of nucleic acid - protein, protein - protein, ligand - receptor, drug - protein target, and enzyme - substrate interactions
蛋白晶元正在成為相當受歡迎，由於他們的未來可能應用在研究核酸蛋白、蛋白質，配體受體藥物靶蛋白和酶基板互動 -
In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。 -
The abstract of a 1999 paper co - authored by jakobsson, samuelsson and two collaborators ends on an upbeat note, saying that the enzyme “ is a potential novel target for drug development
傑可布森、山繆森及另外兩位同事於1999年聯名發表的研究摘要，以樂觀的語氣收尾，說該酵素將是深具潛力的新藥發展目標。
分享友人