template layer 中文意思是什麼
template layer
解釋
臨時層-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
The paper introduces the construction technique and quality control of the concrete ground container, the project uses the small machine - constructing method which vibrates beam and the vibrating spear unify, through example of key working procedure : the construction survey, the ground basic roller compaction, two ash zone constructions, the steel bar construction, the template construction, the concrete surface layer construction craft and the quality control, the small machine construction method prove to meet the project needs
摘要介紹了混凝土集裝箱堆場施工工藝與質量控制，工程採用振動梁和振動棒相結合的小型機械法施工，通過對關鍵工序如：施工測量、場區基層碾壓、二灰層施工、鋼筋施工、模板施工、混凝土面層的施工工藝與質量控制，表明小型機械法施工能滿足工程需要，工程完成後符合驗收標準，滿足了業主的使用要求，對于同類工程的施工具有借鑒意義。 -
Conventionally, this involves coating a silicon wafer with a thin layer of light - sensitive polymer, shining uv light onto it through a template, and then dissolving the affected areas to create a pattern
和傳統方法一樣，先給矽片薄薄地塗上一層對光線十分敏感的聚合物，再隔著一個模板用紫外線照射矽片，然後溶解那些受到影響的區域，這樣就製成了一個圖案。 -
The results showed that in the process of in - situ precipitation cs precipitated layer - by - layer in the template to form 3d layer cs congealation rod by acid - base neutralization of naoh and the amino group of cs. when the process condition was well controlled, it formed homogeneous structure
結果表明：原位沉析法是利用naoh溶液與殼聚糖的氨基起酸堿中和，使殼聚糖分子在模板上原位沉積成三維層狀殼聚糖凝膠棒。 -
This paper reviews the advances in research on the formation mechanisms of ordered mesoporous materials since 1992, and casts light on a few models, such as liquid crystal template mechanism, silicate rod assembling mechanism, silicate layer puckering mechanism, charge density matching mechanism, cooperative formation mechanism, true liquid crystal template mechanism, hydrogen bond - co - - - stack mechanism
摘要簡要介紹了自1992年以來有序介孔材料形成機理的研究進展，重點介紹了幾個重要的反應機理模型，如液晶模板機理模型、棒狀自組裝機理模型、層狀折疊機理模型、電荷密度匹配機理模型、協同作用機理模型、真液晶模板機理模型、氫鍵- -堆積協同作用機理模型等。 -
Based on the concept of " layer and type ", it is designed by layer. the types lies in the layers and then the sub - layers lies in the types. based on the feature type of the parts information, all of the part information is input systematically and finally the information model matched to the process template is created
鄭州大學碩士研究生論文摘要系統信息模型的建立是基於特徵編碼技術，從信息輸入層面的設計入手，按「層、類」思想分層設計，層中有類，類下有層;以零件信息特徵類型為依據，系統地輸入零件各類信息，最後生成與工藝模板相匹配的信息模型。
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