thione 中文意思是什麼
thione
解釋
硫酮-
After the gst fusion proteins were processed by factor xa and were purified by gluta - thione - sepharose 4b affinity chromatography, the recombinant protein hbrp with high purification would be acquired. during the process of separation and purification, the every result which can show the location of the aim protein was monitored by 10 % sds - page. the gst fusion proteins acquired finally were examined and analyzed by the methods of western - blottin
原核表達系統具有表達效率高，操作方便等特點，但缺乏翻譯? 3 ?后修飾，以pgex載體表達的gst hbrp融合蛋白，具有表達的蛋白穩定性好，在體外易於純化等優點，表達的gst融合蛋白可用gluthioneseph刪sc4b進一步純化一factorxa酶切與測分離，從而大量獲得hbrp重組蛋白，而且一次回收率大約在90以上。
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