噬菌體原 的英文怎麼說

中文拼音 [shìjūntǐyuán]
噬菌體原 英文
prophage
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 體 : 體構詞成分。
  • 原 : Ⅰ形容詞1 （最初的; 原來的） primary; original; former 2 （沒有加工的） unprocessed; raw Ⅱ動詞（原...
  1. Bacteriophages are also assayed by a plague assay method based on similar principles.

    基於同樣原理細菌噬菌體也通過空斑測定法進行檢測。
  2. Immunological properties of foreign peptides displayed on a filamentous bacteriophage

    噬菌體展示外源多肽的免疫原性研究
  3. Display of chicken cck protein on the phage t4 capsid surface and its immunogenicity

    4噬菌體表面的展示及免疫原性研究
  4. The monoclonal antibodies ( mab ) specific for the nucleoprotein of prrsv were used to select a phage random heptapeptide library

    採用噬菌體隨機7肽庫對單克隆抗體ge3識別的抗原表位進行了鑒定。
  5. Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use

    X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。
  6. Phage display describes a selection technique in which a peptide or protein is expressed as a fussion with a coat protein of bacteriophage, resulting in display of the fused protein on the surface of the virion, while the dna encoding the fussion resides within the virion

    自1985年gpsmith首次提出噬菌體展示技術以來，隨著生物技術的發展，噬菌體隨機肽庫已成為研究分子間相互作用的有力工具，特別是在抗原表位研究方面。
  7. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  8. This paper describes several latest industrial microbial technologies in detail, which are the synthesis of the chiral diols by epoxide hydrolase from microbie, cofactors regeneration for redox with fdh, production of nano / micro wire by the phage display, metabolic network rebuilding for conventional fermentation and the application of the organic solvent tolerance and the metagenomics technology

    本文綜述了幾項最新的工業微生物技術，主要包括：微生物環氧化水解酶催化合成手性二醇、微生物甲酸脫氫酶用於再生氧化還原反應的輔因子、通過噬菌體展示技術得到納米級金屬絲、代謝網路改造和重建用於傳統發酵生產以及有機溶劑耐受菌和宏基因組技術的應用。
  9. In addition, no back mutation to obtain a maximal complexity ( i. e. - resistant and sensitive species coexistence ) or original ecology ( i. e. original - lysogen ) for the evolution occurs

    而且，逆反應變異回原有菌相而成對噬菌體敏感及阻抗菌相共存之最大分歧度或回歸至起始單一對噬茵體敏感菌相併未曾演化發生。
  10. The amplified phage be used repeat the selection. the selection was repeat three times. the titter of phage indicated that the eluted phage enriched with the rounds increasing

    山西醫科大學碩士學位論文第三次淘洗的噬菌體感染大腸桿菌xli一blue ，鋪制平板，隨機挑取18個分離良好的噬斑，制備噬菌體原種。
  11. In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit

    本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。
  12. And the positive clones were used as the immunogens to immunize mice. both of the serum and phage peptides were add to measure their effects on the growth of huvec ( human umbilical vascular endothelial cell )

    檢驗這種短肽可否模擬vegf的抗原決定簇誘發機體產生能與vegf結合的抗體，研究小鼠抗血清對huvec （人臍靜脈血管內皮細胞）生長的抑制作用以及噬菌體表達的7肽和12肽對huvec生長的抑制作用。
  13. Thirteen putative epitopes showing characteristics of antigenic epitope were found from the analysis information. using pcr, the nucleotide acid fragments encoding these putative epitopes were amplified, then cloned into the expression vector miske. the positive recombinant phage displying the epitopes were found out by using pcr, sequencing and the determination of phage plaque titer

    運用goldenkey分子生物學軟體對prrsvbj - 4結構蛋白的抗原表位及其二級結構進行了分析和比較，從中篩選13段顯示表位特徵的氨基酸殘基序列，用pcr技術擴增相應的核苷酸片段，將其插入到噬菌體表達載體m13ke ，結果預測的13個表位可在噬菌體表面得以展示。
  14. The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis

    取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。
  15. In order to provide valuable data for further exploring the immunogenicity and function of structural proteins of prrsv and designing epitoe vaccine for prrsv, the purpose of the research was to characterize the epitopes on structural proteins of prrsv bj - 4 by means of phage display technique, elisa and gene expression etc. 1. the epitopes and secondary structures of the structural proteins of prrsv bj - 4 were forecasted by molecular biology software goldenkey

    本研究利用噬菌體展示技術，結合elisa和基因表達等技術對prrsv結構蛋白的抗原表位進行了鑒定與分析，為prrsv的結構蛋白免疫特性與功能研究以及表位疫苗的設計等奠定了基礎。
  16. Detection of lysogenic phage in the culture of symbiotic xenorhabdus and photorhabdus bacteria and solid culture system of entomopathogenic nematodes

    昆蟲病原線蟲和共生細菌培養系統中噬菌體的檢測
  17. Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii, the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa

    3 mg _ 7重組噬菌體抗體庫的淘篩用m13ko7輔助噬菌體感染轉化菌，以挽救出噬菌體形式的抗體庫；用高表達mg _ 7抗原的胃癌細胞系kato對抗體庫進行三輪淘篩；用elisa篩檢呈現有mg _ 7scfv的噬菌體單克隆。
  18. In addition, the display of repertoires of antibody fragments on the surface of filamentous bacteriophages offers a new way of making antibodies with predefined binding specificities

    高親和的抗乙肝病毒表面抗原pres1 （ 20 - 47 ）抗體具有封閉hbv的作用。噬菌體展示技術提供了獲得結合特異表位抗體的新途徑。
  19. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。
  20. The antibody induced by peptide gwyydal abolished vegf - induced huvec growth in dose dependent which specifically express the kdr. moreover, both of the peptide gwyydal and vasavfysalve displaying on phage also inhibited the growth of huvec

    [結論]從噬菌體肽庫中篩選到了與抗體有高親和力的陽性噬菌體克隆，噬菌體表達的7 、 12肽gwyydal 、 vasavfysalve模擬了vegf的抗原表位，引起顯著的抗- vegf抗體反應。
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