噬菌體雜交 的英文怎麼說
中文拼音 [shìjūntǐzájiāo]
噬菌體雜交
英文
phage cross-
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions, clearing aged cells and metabolic products, regulating immune responses and protecting against infection. in some pathological states such as psoriasis and contact dermatitis, a certain serum level of the antibody could inhibit the progression of the diseases, and is beneficial to the recovery from the diseases. after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies, meanwhile, experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) ", a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability, immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis, and recombinant antibody is under investigating
抗角蛋白自身抗體（ akautoab ）是naa的重要組成部分，以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，並對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究，直接或間接地發現akautoab是機體正常免疫調節網路的組成部分，在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用；在某些病理情況下（如銀屑病、接觸性皮炎等） ，體內akautoab的組分和滴度會發生變化，而正常水平的akautoab則有利於限制病情的發展，促進損傷的修復。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。
分享友人