It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation
最佳氮源為玉米漿和蛋白腖,最佳比例為2 : 3 ,最佳濃度為1 . 6 ;加入其它碳源時有助於菌株穩定產酶; 100ml搖瓶的最佳裝液量為15ml ;肌酸、肌氨酸和氯化膽
堿都能誘導菌株產酶,其中肌酸誘導產酶的效果最好,而肌
酐和尿素不能誘導菌株產酶;誘導物肌酸的最適加入時間為接種培養12小時后,最適加入量為0 . 75 。
The resin of alkali developable photosensitive has been synthesized from the raw material of acrylic acid, maleic anhydride and epoxidized soybean oil
摘要以環氧大豆油、丙烯酸和馬來酸酐為原料合成了堿溶性光敏環氧丙烯酸酯齊聚物,用紅外光譜對產物進行了表徵。