塗抹培養 的英文怎麼說
中文拼音 [túmǒpéiyǎng]
塗抹培養
英文
smear culture- 塗 : Ⅰ動詞1 （抹） spread on; apply; smear 2 （亂寫或亂畫） scrawl; scribble 3 （抹去） cross [blot; s...
- 抹 : 抹動詞1. （用抹子弄平泥或灰） daub; plaster 2. （緊挨著繞過） turn; bypass; skirt
- 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
- 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
- 塗抹 : 1. （塗在東西上） daub; smear; paint2. （亂寫; 亂畫） scribble; scrawl
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Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle
首先用粗略制備的原生質體經紫外誘變篩選到一株酶活為2250u ml的實驗出發菌；制備成菌懸液，紫外燈下照射誘變，紅光下塗抹篩選平板，恆溫培養2 3d ；按透明圈大小進行預初篩，挑選徑圈比大的菌落接斜面，恆溫培養3d ；按一株一瓶的方式進行初篩；從中選取酶活較大的3 5株，按一株2 3瓶的方式進行復篩；挑取酶活大且穩定的1 2株進入下一代誘變篩選。
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