巰基組氨酸 的英文怎麼說
中文拼音 [qiújīzǔānsuān]
巰基組氨酸
英文
thiolhistidine-
Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on
該蛋白質中含有保守的- cp ( g ) pc -氨基酸活性基序,該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞,對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連, trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。One 66kd band appeared except 44kd main band when go isozyme above was subjected to sds - page and ce - sds, indicating this go isozyme was similar to that from spinach leaves which contained 40kd and 66kd simultaneously. whether b - mercaptoethanol was added or not when go isozyme was subjected to in sds - page and ce - sds, 40kd main band and 66kd band still appeared, indicating two subunits were not linked by covalent disulfide. amino acid analysis shew that the ratios of basic to acidic amino acid of go isozyme and its 40kd acidic subunit were 0
菜心go同工酶的sds - page和sds -毛細管電泳( ce - sds )顯示,該酶除了含40kd主帶外,還有很淺的66kd帶,和之前我們提出的菠菜go同工酶含40kd酸性亞基和66kd堿性亞基相似; sos - page和ce - sds電泳中,無論加入-巰基乙醇與否, go同工酶都只有40kd主帶和66kd淺帶,表明菜心go同工酶中40kd酸性亞基和66kd堿性亞基不是以共價二硫鍵相連;用制備性sds - page法獲得菜心go同工酶的40kd亞基,並和菜心go同工酶一起測定其氨基酸組成,該go同工酶及40kd亞基的堿酸性氨基酸的比例分別為0 . 66和0 . 54 ,表明40kd亞基可能是個酸性蛋白,而66kd帶則是個堿性蛋白。
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