接受序列 的英文怎麼說
中文拼音 [jiēshòuxùliè]
接受序列
英文
receiving sequence-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。The reason of stopping flying of large scale aeroboat in china may due to the complex application of the permission, flying line approval, expensive cost and professional maintenance in addition to the technical problems, the cost of 12 thousand us dollars roughly equal to rmb 100 thousand and the flying time of at least 15 days is not easily acceptable by any normal enterprises
大型商業載人廣告飛艇在國內停飛除技術原因外，飛行手序的申報，航線申報等一系列繁瑣的程序，昂貴的飛行費用，專業的飛艇維護，眾多的場地租賃都限制了它的大眾化。每飛行日1 . 2萬美元合10萬元人民幣，最少飛行15日，非一般企業所能接受。Direct sequence - code divition multiple access ( ds - cdma ) technology is achieved easily and used widely at present its receiver capability is confined by two factors, one of which is the multiple access interference ( mai ) which makes the capacity low, another of which is the near - far problem
直接序列擴頻-碼分多址（ ds - cdma ）技術是最易實現、也是目前應用最廣的一種方式，它的接收性能受到兩個主要的限制：一個是多址干擾造成的性能下降；另一個則是遠近效應問題。I have read and understand the conditions of quarantine surveillance detailed as listed in part c above and i undertake to comply with these conditions, acknowledging that compliance is necessary for the post - arrival quarantine to be served as quarantine surveillance
本人已閱畢及明白上述丙部所列的檢疫監察條件，並承諾予以遵守，而本人確知必須遵守有關條件，才可讓該犬只接受檢疫監察，作為其抵港后的檢疫程序。Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product
用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。( 5 ) user management system is built by asp technology on the server - side. user ' s register and logging is accepted and the user permission for different group is designed ( program files referred to appendix 1 ) ; the connection between web server and plastic die material database system realized by ado, odbc technology, which will guarantee the mutual information transportation between the database system and different terminals ; ( 6 ) further study of expert system is discussed and the implementing reasoning base on plastics mould material selection neural networks is put forward and key problems is also discussed. according to the requirements of sustainability, extended knowledge frame of mould selection and track algorithm meeting the requirements of dynamics, history, structuralism, illegibility, unintegrity and uncertainty are verified and corresponding examples are also put forward
（ 3 ）通過ado 、 odbc技術實現web服務器與塑料模具材料數據庫系統的連接，從而保證了塑料模具材料數據庫系統和各終端之間信息的雙向傳遞； （ 4 ）在服務器端利用asp技術建立塑料模具材料遠程選擇優化專家系統交互界面，通過標準數據庫查詢語言，客戶能對數據庫中的塑料模具材料信息進行查詢（程序文件列表參見附錄1 ） ；廣東丁業人學1學m學位淪義門）在服務器端利用sp技術建立用戶管理系統，接受用戶的注冊、登錄消求，為不同等級的用戶設定相應的權限（程序文件列表參見附錄1 ） ； （ 6 ）討論了進一步完善該專家系統所需做的工作，提出塑料模具選材神經網路批理的實現方案，並對其中的關鍵問題進行了討論：按照可持續發展的要求，對擴展的模具選材知識框架、滿足動忐性、歷史性、結構性、模糊性、個大根性、不硯定性等需求的跟蹤演算法進行了論證並給出計算示例。A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。Other than traditionally theory analyze and discussion of digital correlator which is laid after demodulator, a scheme based on non - interrelated correlative dll will be discussed in this paper after referencing the theory and the result of simulation. the demodulated data and the information of carrier are not need in this plan. we sample the result of correlation between local sequences and the received signal, then realizing the code synchronization by software
不同於一般的理論分析以及解調后數字相關器的討論，本文通過理論分析與模擬結果，選用了非相干相關同步的基本結構，並加以改進，不需要解調和載波的信息，將本地序列與接受信號直接相關后進行采樣，通過軟體的方法，實現同步。After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。Then, inspired by the idea of evaluation method we propose a new method and construct a simple music structure based music information retrieval system, the experiment results show that it is a perspective method, the system can give a list of retrieval results ordered by structure similarity that extracted from the automated music structure label system build up before, and two same music piece can be identified almost 100 %, so it is very useful in mir and intelligent play list generation
受到結構評價方法啟發，本文又提出了基於結構分析的音樂檢索的方法，初步探討了利用音樂結構信息建立檢索系統這一新方法的前景。初步實驗結果顯示這種方法是很有前途的，它可以直接檢索音頻形式的音樂，按照結構相似程度給出最後結果並排序輸出，這在音樂檢索和智能播放列表的生成方面都有廣泛的應用。To many scientists, epigenetics amounts to a heresy, calling into question the accepted view of the dna sequence ? a cornerstone on which modern biology sits
對許多科學家來說，實驗胚胎學就像是異端邪說，因為它質疑已被人們接受的關于dna序列的理念，而後者正是現代生物學的基石。In may 2002, the author was appointed to manage the dacheng hotel by sichuan demotic congress. he had guided the staff to carry out a series of effective measures successfully, holding the mind of sincere, devoted, holding together and learning to construct the culture of the hotel. they still strengthened the managing thought that the customers who were at the first place should be serviced at pains. they kept establishing and perfecting all kinds of bylaw and job prescribe so as to assure the effective, in - order, normative running
2002年5月，筆者受省人大辦公廳委託，開始全面接手對大成賓館的管理經營工作，帶領全體員工，臥薪嘗膽，艱苦創業，實施了一系列有效的改革措施，始終堅持以「誠信、敬業、團結、學習」的企業文化建設為核心，強化「用心服務、顧客第一」的經營理念，堅持建立和完善各項規章制度、工作規范，保證企業高效、規范、有序的運行;堅持「以人為本」的人本思想，充分調動全體員工的工作積極性。Members of the volunteer service are trained to perform emergency duties during typhoons, landslips and flooding ; to search for and rescue people in distress in the mountains ; to help evacuate or rescue victims trapped under collapsed buildings or buried in landslides ; to combat forest fires and oil pollution at sea ; to carry out crowd control duties and to provide communication services at charity functions and government events
民安隊隊員均須接受訓練，以便執行下列任務：在臺風襲港、山泥傾瀉或水災時奉命出動；搜索並拯救困於山野的人士；協助正規部隊拯救埋于倒塌樓宇或山泥中的人士；撲滅山火和清理海上油污；在慈善或政府活動中維持秩序和提供通訊服務。Volunteers are trained to perform emergency duties during typhoons, landslips and flooding ; to search for and rescue people lost, injured or trapped in mountains, collapsed buildings or buried in landslides ; to combat scrub fires and oil pollution at sea ; and to carry out crowd control duties and provide communication services at charity functions and government events
隊員均須接受訓練，以便執行一系列緊急任務：在臺風襲港、發生山泥傾瀉或水災時奉命出動；搜索拯救困於山野、倒塌樓宇或山泥中的人士；撲滅山火和清理海上油污；並於慈善或政府活動中維持秩序和提供通訊服務。For example, if a microsoft excel spreadsheet consists of the resource name and resource cost columns, that spreadsheet can accept only text and numerical information in that order
譬如，如果一個microsoftexcel電子表格包含「資源名稱」和「資源成本」列，則該電子表格只能接受相同次序的文本和數值信息。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。In general, when people are referred to a clinical psychologist, a thorough and objective psychological assessment will be conducted to determine the nature of the problem, and the kind of assistance that will be appropriate. where necessary, a treatment plan can be formulated
臨床心理學家會因應接受服務者的問題，作出一系列有系統及客觀的心理評估和分析，然後制訂心理治療的程序，以幫助當事人改變對事物的觀點、感受及情緒反應，提高個人的適應能力及消除心理困擾。Recently spread spectrum technique has been used widely in wireless data communication, cellular mobile communication, military communication and telemetry monitor system with resistance to interference, high usage of spectrum, resistance to multi - path fading, so investigators in communications field pay more attention to the technique, especially direct sequence spread spectrum ( dsss ) technique
近年來，擴頻技術以其抗干擾能力強、頻譜利用率高、抗多徑等優越性能在無線數據通信、蜂窩移動通信、軍事通信以及遙測監控系統中獲得了廣泛的應用，並日益受到通信領域研究人員的重視，特別是直接序列擴頻技術（ dsss ） 。The families and genera are indexically listed
名錄中接受的科和屬按字母順序索引列出。分享友人