插入序列 的英文怎麼說

中文拼音 [chārùxùliè]
插入序列 英文
insertion sequence
  • 插 : 動詞1. （把細長或薄的東西放進、擠入、刺進或穿入; 插上; 插進） stick in; insert 2. （中間加進去; 加進中間去） interpose; insert
  • 入 : Ⅰ動詞1 （進來或進去） enter 2 （參加） join; be admitted into; become a member of 3 （合乎） conf...
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 插入 : insert; infix; run in; break in; patch; insertion; plug in; intercalate; intercalation; intromiss...
  1. Floral aberrance of cbf1 transgenic tobacco and analysis of its flanking sequences

    1基因煙草植株的花器官變異及其插入區側翼序列的分析
  2. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  3. The results showed that the f fragment, 728bp in length, could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment, about 4kb in length, is inferred to have repeat sequences around tn5 insertion site, in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica. to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals, the experiment with mice was carried out

    結果顯示， f片段長度為728bp ，與現有生物數據庫的blast比較分析，發現該序列僅有局部短於1oobp的區域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性，推測為一新基因； b片段長約4kb ，序列拼接結果推測靠近tn5插入位點部位有重復序列，對b片段tn5遠端的部分序列進行blast比較，發現它與小腸結腸炎耶爾森氏菌的強毒力島有一定的同源性。
  4. Dna sequence analysis indicated that tn5gusa5 is prone to insert into low gc content regions ; guanine is a preferential base at the first place and cytosine at the last site of target sequence

    在質粒上tn5gusa5也傾向于插入低gc含量區；堿基g和c分別在靶序列的首位和末尾出現的幾率高。
  5. Some tendency of tn5gusa5 transposition were found that all preferred sites of tn5gusa5 in xcc 8004 genomic dna are in at - rich regions ; target sequences of tn5gusa5 have some features that the probabilities of guanine and cytosine are high respectively at the head and tail base of target sequence ; the level of gene transcription does not influence insertion density of tn5gusa5 significantly

    結果表明， tn5gusa5插入位點有一定的規律性： tn5gusa5在xcc8004基因組上傾向于插入低gc含量（ 50左右的區域插入密度最高）區段；插入位點的靶序列有一定的特異性，在靶序列的首位鳥嘌呤出現的幾率高，而在靶序列的末位胞嘧啶出現幾率高； tn5gusa5的插入密度與該區段基因的轉錄水平無明顯關系。
  6. Transposable elements, or insertion elements, are dna sequences which can be inserted into many different sites in chromosomes.

    轉位因子或插入因子是能夠插入到染色體不同位點的DNA序列。
  7. Transposable elements, or insertion elements, are dna sequences which can be inserted into many different sites in chromosomes

    轉位因子或插入因子是能夠插入到染色體不同位點的dna序列。
  8. Compared with the 5. 8s complete sequence of the snail arion rufus, its1 and its2 regions were recognized and combined for analysis. from sequence observation, it showed that the zhejiang sample has more inserted sites and fragments while the sequences of other three are nearly all the same. the average g % + c % of the four individuals was 46. 8 % while the zhejiang sample ' s was 48. 3 % and the other three ' s were all about 46. 2 % ; ts / tv and genetic distance mainly lies between the zhejiang sample and the other three individuals, which were 0. 8 and 0. 07 respectively

    用於比較的序列長約350bp ，觀測一級結構，加拿大、墨西哥灣扇貝和美國二代個體的its1和its2序列幾乎完全相同，而浙江個體則具有較多的插入位點與片段； 4個個體平均g + c含量46 . 8 % ，其中浙江個體為48 . 3 % ，其它3個個體均為46 . 2 %左右；轉換顛換比與遺傳距離主要存在於浙江個體與其它3個個體之間，分別為0 . 8和0 . 07左右；以櫛孔扇貝作外群構建的分子系統樹表明：浙江群體已產生了一定的分化。
  9. Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions

    細胞色素b基因序列變異分析表明： 1 ）雁形目鳥類細胞色素b基因全序列長度一致，無插入和缺失：對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列，無無義突變，全序列內部無終止密碼子； 2 ）序列比對后1143加，含416個核著酸變異位點， 306個簡約信息位點，其中處於密碼子第三位的變異最大，第一位和第二位堿基的變異相對較小。
  10. We start with an empty list, and we insert a 0 if the first bit is 1

    開始時序列為空，如果第1個比特是1 ，我們就插入一個0 。
  11. However, the cloned promoter had 18 substitution at 18 sites, 22 deletions at 18 sites and 3 insertion at 3 sites between sites 0 - - - 1273 bp which was reported to control temporal - spatial specific expression, and 3 substitution at 3 sites, 6 deletions at 6 sites between - 1095 bp and - 1273 bp, the key functional sequence area, in comparing with known osg6b

    但是與報道的osg6b比較，在決定時空特異性的0 - 1273bp功能區域內，有18處出現18堿基替換， 18處出現22堿基缺失， 3處出現3堿基插入；在核心功能序列區域（ - 1095bp - 1273bp ）內，有3處出現3堿基替換， 6處出現6堿基缺失。
  12. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  13. It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain

    通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段，將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較，結果在參比的16個ndv毒株中在該區段中除了有多個點突變外，個別毒株有堿基插入和缺失，所有以lasota株為代表的弱毒株均無6堿基的插入，而以f48e9株為首的強毒株均有此6堿基的插入，但有一個中等毒力的毒株dp沒有6堿基的插入，不過它的基因序列和lasota的幾乎相同，對于所克隆到的基因的代表性還有待確定。
  14. Transformation efficiency is greatest with circular plasmids containing only small inserts of passenger dna.

    使用帶有一小段客座DNA的插入序列的環狀質粒進行轉化，較率會最高。
  15. Selenocysteine insertion sequence, secis

    硒半胱胺酸插入序列
  16. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  17. The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod

    結果表明同源性分別達到98和97 ，並且在ha1切割位點有多個堿性氨基酸的插入序列，證明其為強毒株。
  18. Osdd2 gene existed as a single copy gene in the rice genome based on the result of southern blot analysis. we took a pcr method to clone the cdna of osdd2 gene

    使用克隆的邊端插入序列為探針對水稻（中花11品種）進行southern雜交分析，表明osdd2基因在水稻基因組中是以單拷貝存在。
  19. Transformation efficiency is greatest with circular plasmids containing only small inserts of passenger dna

    使用帶有一小段客座dna的插入序列的環狀質粒進行轉化，較率會最高。
  20. Methods : the two pairs of designed primers were based on pzp3 a and hcg p - ctp109 - 145 cdna sequences. the pzp3 a - hcg p - ctp109 - 145 chimera was amplifiled by overlapping pcr. the chimera was cloned into ppic9k plasmid and transformed into e. coli dh5 a

    結果： 3步pcr擴增出pzp3 - hcg - ctpdna片段，插入到載體質粒ppic9k的克隆位點，獲得重組ppic9k - pzp3 - hcg - ctp表達質粒，測序結果顯示插入序列與設計預期完全一致。
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