整合基因 的英文怎麼說

中文拼音 [zhěnggějīyīn]
整合基因 英文
integrator gene
  • 整 : Ⅰ形容詞1 （全部在內; 完整） whole; all; complete 2 （整齊） neat; tidy; orderly Ⅱ動詞1 （整理; 整...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 整合 : commensuration
  1. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  2. Transformed shoots were selected on solidified medium containing kanamycin. ten kanamycin resistant transformants were obtained by direct or induction calla. these transformants were checked by pcr, pcr - southern blot and southern blot, confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad

    對所得到的抗性轉化株進行了pcr 、 pcr - southernblot 、 southernblot檢測，其中2株呈陽性，證明vp4基因已整合到苎麻基因組dna中。
  3. The global regulator csra of e. coli is a specific mrna - binding protein. csra negatively regulates several metabolic pathways that are induced post - exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism ; positively controls gene expression within the glycolytic pathway ; and also csra modulates the levels of enzymes that participate directly in pep metabolism

    Csra是整體調控網路的調控基因，可負調控指數生長後期誘導的一些代謝途徑，包括糖原的生物合成、糖原的分解代謝、糖異生，而對糖酵解的一些重要基因的表達則執行正調控功能， csra也調控直接參與pep代謝的三個酶的活性水平。
  4. By applying the th model theory to analyzing the cases of developed countries that mentioned in chapter 4, chapter 6 finds out the block gene and tendon compages that consists of the university - government - industry th. furthermore, it gives the pheno - types as some external expressions of the genotypes and the family - tree diagram constituted by these phenotypes. after a transition, it illuminates the scientometric, the webometric and the triple helix algorithm and their application

    第六章運用th模型理論對第四章提供的案例挖掘梳理后，發現並給出了：構成大學?政府?產業三重螺旋體的完整板塊配基和鍵鏈組合，作為「基因型」外在表達的一些「現象型」屬類以及由它們所構成的圖式譜系。
  5. In the article we study the venture of the end stage of m & a. firstly, we clear the definition of merger and acquisition, m & a risk, venture discernment and so on ; secondly, introduce the status quo, analyse the acute tide on m & a in west country, get the enlightenment and find some problem to china enterprise ; analyse and epurate the six aspects, thirteen factors, and design thirteen corresponding indexes. then the coherent coefficient of integration is set up by fuzzy method and accurate value measurement

    本文的基本思路是：首先澄清相關的基本概念，如並購，並購風險，並購風險識別等；第二，介紹了企業並購的現狀，通過對西方國家風起雲涌的並購浪潮的分析，由此對我國並購的啟示，進而分析我國企業並購現存的主要問題，從而對企業並購整合加以界定；第三，對企業並購整合類型加以分析和提煉，總結出並購整合可以分為六大類型， 13個影響因素；最後，針對整合的六大類型、 13個因素，分別運用不同的方法，定性與定量相結合，對它們進行識別。
  6. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  7. Transgenic integration site detection is the efficient method to identify whether the transgenic crops are approved to import or export. some quantitative detection methods were reported in 2000 and 2001, and that is about bt11 and mon810 corn. real - time 5 " - nuclease pcr had been previously used successfully to determine quantitatively roundup ready ( rr ) soy and btl76 maize in food

    轉基因整合位點檢測是鑒定轉基因品種是否為批準進口轉基因作物的有效的特異性方法，國外在2000年和2001年已開展了轉基因玉米bt11 、 mon810兩個品種整合位點的特異性檢測方法研究。
  8. However, in the late phase of the growth, the strain hv grew a little faster than e. colihms174. hv ( ptz101 ) was constructed by the plasmid containing the phb operon and the parde gene tansformed into the strain hv

    將帶有phb操縱子和質粒穩定分配基因parde的質粒ptz101引入vhb整合菌中，構建成為產phb的vhb整合菌hv （ ptz101 ） 。
  9. Blast result showed the fragment containing the opuaa and its 5 " upsteam sequence was obtained

    通過blast比較，表明獲得完整的atp結合蛋白基因orf框序列及其上游的核苷酸序列。
  10. Results of pcr for the resisted shoot and transgenic plants showed that bt gene had intergrated into maize genome

    對抗性芽以及再生植株的pcr檢測初步證明， bt基因已整合進玉米基因組中。
  11. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  12. The individuals of rhd - positive phenotype with intact exons carried generally insert fragments and boxl box2 and box3 and this proved that inserts or rh box could n ' t affect the express of rh d gene. in 2 of the 5 wei nationality pedigrees whose proband were rh d - negative, rhc / e phenotype of all the rh negative individuals was ccee. rhd exon 4nsert and rh box did not be found in all individuals

    在7個先證者為rhd陰性的漢族家系中，大部分成員均出現插入片段和rhbox ，且在遺傳上符合孟德爾遺傳定律， d外顯子完整且表型為rhd陽性的家系個體成員廣泛帶有插入片段和box1 、 box2或box3 ，插入片段或rhbox並未影響d基因的表達。
  13. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  14. Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch

    利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。
  15. Now it has been one of the most important aquatic products in the freshwater cultivation. however, this prawn ca n ' t survive at a water temperature lower than 14c, which has seriously limited its cultivation expanding. in order to obta in a new breed of this prawn with increased cold - resistance, we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ), a sperm - mediated gene transfer technique

    本研究的特色和創新之處在於，針對羅氏沼蝦不耐低溫，但體型相對較大，精莢明顯的特點，首次將目前已知具有最強抗凍活性的雲杉卷葉蛾（ sprucebudworm ， choristoneurafumiferana ）抗凍蛋白（ sbwafp ）基因（ sbwafp ） ，通過精子介導的轉基因技術整合到羅氏沼蝦的胚胎中，以期培育出耐低溫的羅氏沼蝦新品系。
  16. In view of existing situation of eco - environment in the west, this thesis divides it into five different zones, according to the principle of suit measures to local conditions, designs different administering modules of forestry ecology ; hi view of existing situation of industrial structure in the west, this thesis discusses specific forestry s contribution on restructuring of agriculture, industry and the third estate

    針對目前西部生態環境現狀，合理劃分區域，按因地制宜，分區治理原則，設計適合各區特點的林業生態治理模式：針對日前西部產業結構現狀，提出產業結構調整的基本思路，具體論述了林業對西部農業、工：業及第三產業結構調整所應發揮的作用。提出了各自相應的對策與措施。
  17. Css is the one of the colonization factor antigens which is a protective antigen can cause immune reaction by the means taking orally. in this study, carrot was separately transformed with agrobacterium tumefacience strain lba4404 that contains ctb or ctb - cs3 fused gene in order to get oral diarrhea vaccine. this is a potent strategy to produce etec oral vaccine

    本研究期望通過農桿菌轉化系統將ctb （霍亂毒素b亞基，可作為佐劑和載體）基因和ctb - cs3融合基因分別轉入胡蘿卜植株，使ctb和ctb ? cs3融合基因穩定整合到胡蘿卜基因組內，希望以此獲得以胡蘿卜為受體的etec口服疫苗，使服食者在進食的同時就可獲得腹瀉免疫。
  18. On the basis of that, through making use of dynamic game model to analyze the reason of integration of high - tech industry of mainland and taiwan, finds the nash equilibriums of technique cooperating degree of high - tech industry of mainland and taiwan under the same or different quality technique level condition, and uses the current situation of high - tech agriculture experiment area of fujian and taiwan to examine it, then utilizes anterior conclusions to give the countermeasures to the regulation agency of mainland and taiwan in high - tech industry cooperation

    在此基礎上，通過運用動態博弈模型對海峽兩岸高科技產業的整合動因進行分析，找出海峽兩岸高科技產業在同質技術水平與異質技術水平條件下海峽兩岸高科技產業技術合作意願度的均衡點，並用閩臺兩地高科技農業試驗區的情況進行了一些實證檢驗，然後利用前面的分析與模型得出的結論，針對海峽兩岸的管理層在高科技產業合作方面提出了相應的對策。
  19. 2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin

    二、在以上研究的基礎上，對cmiv抗菌肽的c端進行較大的改造，即將與腫瘤細胞過度表達的表皮生長因子受體（ egfr ）具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端，設計完整的基因，並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。
  20. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
分享友人
分享到 Line

分享到臉書