明達克 的英文怎麼說
中文拼音 [míngdákè]
明達克
英文
mindak-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。It was very different in the early days of the occupation of southern iraq when british soldiers patrolled in soft berets, in contrast with the americans in baghdad
英軍接管伊拉克南部地區初期，只需戴著貝雷帽巡邏，與在巴格達的美軍形成了鮮明的對比。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Mountbatten then capped matters by drawing a pistol from his pocket to demonstrate the strength of pykrete against gunfire.
蒙巴頓從衣袋中掏出手槍，準備用它來證明「派克里特」抵抗槍炮的力量，這就使表演達到了高潮。These results indicated that the cloned osg6b " had a constitutive characteristics but not anther specificity, and the transgene was segregated in a mendelian fashion in the tl generation
研究表明，所克隆的osg6b 』不具有花藥特異性，而具有組成型表達的特徵。第二部分：從大腸桿菌eWithin 700 km, there are 8 european capitals around kielce - berlin in germany, vienna in austria, prague in czech, minsk in belarus, kyiv in ukraine, bratislava in slovakia, vilnius in lithuania and budapest in hungry
其周邊700公里內就輻射到了八個國家的首都? ?德國柏林、奧地利維也納、捷克布拉格、白俄羅斯明斯克、烏克蘭基輔、斯洛伐克布拉迪斯拉發、立陶宛維爾紐斯、匈牙利布達佩斯。Use 3 - 5 adjectives to describe aladar ， kron ， neera and bruton. then tell the reason
用3 - 5個形容詞來描述一下艾力達、克隆、妮娜和布魯頓，然後說明理由。Root distribution and canopy structure parameters of s. godejevii in different sandy land habitats ( semi - fixed sand dunes, fixed sand dunes and inter - dunes low land ), as well as species composition and their frequency in the herb layer of the scrubs, were compared the results were as follows : the population hi the semi - fixed sand dunes had the most developed root systems, followed by he population in the fixed sand dunes, and that hi the inter - dunes low land was the last
為了更有效地利用這一重要植物資源，本文對分佈於渾善達克沙地三種不同沙地生境（半固定沙丘、固定沙丘、丘間低地）黃柳灌叢的根系分佈，冠層特徵進行了研究，同時測定了灌叢下草本層植物種類組成及頻度，結果表明：半固定沙丘上的黃柳根系最發達（根系分佈深，數量大） ，固定沙丘的黃柳明顯不及前者，而丘間低地的黃柳根系最不發達。Western - blotting result demostrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody. our system improve the expression level of r hpf4 by 80 fold compared with pt7 - 7 - r hpf4. after purified and renatured, r hpf4 prepared by our methods has bioactivity like wide hpf4. our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells
我們構建的rhpn原核高效表達系統經m page及凝膠密度掃描分析結果表明， rhpf4表達量占菌體總蛋白量的25 30 ，較原表達克隆pt7 7 rhpf4提高了近80倍，經快速高效的包涵體分高純化工藝和復性工藝， rhpf4具有野生蛋白活性。By using western - blot, the fusion protein could not be react with antiserum to prrsv, whereas it presented a reactivity with swine antisera against prrsv and mab ge3 by elisa the results implied that the epitope might be one conformational epitope that was mainly composed of aa50 ~ aa55 domain on n protein
Western - blot分析表明表達產物與prrsv陽性血清沒有反應性，而elisa分析表明表達產物可與prrsv陽性血清和單克隆抗體發生反應。由此說明單克隆抗體ge3所識別的抗原表位可能是存在於n蛋白上以kphf為核心的構象型表位。Such credit or refund shall, if applicable, be requested by written notice to nasdaq with all pertinent details
若是適用，應向那斯達克以書面方式並載明細節，提出信用額度或退款要求。A survey showed that hundreds of internet companies failed to meet the basic requirements for being listed at nasdaq and that they might be removed from trading at any time
一項調查表明，有幾百家網路公司不符合在納斯達克交易所掛牌的起碼要求，有隨時被摘牌的危險。Phaa, phab and phac were inserted to pbv - 220 with double digest of restriction enduonuclease. the expression vectors of pbv - a, pbv - b and pbv - c were constructed by orientaional cloning. indefication of expression vector with restriction enduonuclease digest showed that phaa phab and phac were in right orfs
將phaa 、 phab和phac片段雙酶切后，定向克隆至原核表達載體pbv220 ，構建了三個原核表達載體pbv - a 、 pbv - b和pbv - c ；經酶切分析表明，所克隆的三個基因phaa 、 phab和phac置於表達載體的正確閱讀框架下。On the 10th of october dohturov had marched halfway to fominskoe, and halted at the village of aristovo, making every preparation for exactly carrying out the orders given him. on the same day the whole french army, after reaching in its spasmodic rush as far as murats position, seemingly with the object of giving battle, suddenly, with no apparent cause, turned off to the left to the new kaluga road, and began marching into fominskoe, where broussier had before been alone
十月十日，多赫圖羅夫前往福明斯克途中，抵達阿里斯托沃村，停止前進，準備正確執行上級命令的時候，就在這同一天，好像得了瘋病一樣，全部法國軍隊開到了繆拉的陣地，好像準備要打一仗，可是突然又無緣無故地向左轉到新卡盧日斯卡雅大路，進駐原先只有布魯西埃駐扎在那裡的福明斯克。Manchester united have agreed an undisclosed fee with spartak moscow for defender nemanja vidic, the premier league club announced on its website
效力于莫斯科斯巴達克隊塞黑國家隊著名后衛維蒂奇將在明年1月1日球員冬季轉會期開啟之後加盟英超曼聯。Starkey group is a global corporation with more than 4, 000 employees worldwide, its headquarter located in minnesota usa
斯達克是一個全球發展的集團公司，總部位於美國明尼蘇達州，在全世界范圍內擁有超過4000名的雇員。Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage
構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。Darkstorm has proven time and again that any who challenge him meet a quick end
達克斯通已經多次證明過任何膽敢挑戰他的人都會死得難看。The council urges that the government should, having regard to the characteristics of the hong kong financial market, expeditiously review the risk management of information technology - related stocks and adopt effective measures as soon as possible to develop an excellent culture of corporate governance, so as to enhance the vitality of the hong kong investment market, protect the legitimate rights and interests of investors and provide a level playing field for professional intermediaries in the market time to review the risk management of tech stocks
當時本港的投資者，由盈動提出收購電訊一役開始，正對新興經濟市場的發展，抱著極高的期望，甚至乎，在剛啟業的創業板上市的十數只科技股，亦備受高度追捧，形成了股價超高的現象，而美國的納斯達克市場，也已經累積了長時期的超買，科技股明顯地正處於一個等待著調整的階段。The sponsorship for the beijing olympics is exclusive, says chen luming, olympic project manager for sohu, which is traded on the nasdaq stock market
「北京奧運會的贊助商資格是獨家性質的， 」搜狐（納斯達克上市公司）奧運項目經理陳陸明表示。分享友人