核酸分離法 的英文怎麼說

中文拼音 [hésuānfēnlífǎ]
核酸分離法 英文
nucleic acid separation method
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  1. When using the nuclear acid sequence and the predicted amino acid sequence of scghr to blast the fugu genome, we found that there were two contigs which shared the most similarity with scghr. therefore we realized there maybe were two ghrs encoded by different genes in teleosts. then, by means of nuclear acid sequence joining, orf of fghr1 and fghr2 were predicted from fugu genomic sequences using cohoghr isf1, cohoghr isf2, chghr and other related ghr amino acid sequence available at ncbi database

    當利用南方鯰ghr的核苷酸、氨基酸序列blast東方?的基因組時，發現有兩個contig與其氨基酸序列非常相似，因此意識到魚類可能存在兩種不同基因編碼的ghr 。隨後主要利用已經克隆得到的cohoghrisf1 、 cohoghrisf2 、 chghr以及其他魚類ghr核苷酸和氨基酸序列blast東方純的基因組，採用序列拼接的方法從東方?基因組中分離出兩種不同基因編碼的fghr1和fghr2 （本研究把和傳統ghr相似的叫做ghr1 ，把另外一種叫做ghr2 ） 。
  2. Meanwhile, these six coat protein genes were sequenced and compared with other homologous sequences in genbank. the strain designation of the six isolates of tumv was finally determined. the results are as the following : 1. six isolates of turnip mosaic virus named tumv - sd1, tumv - sd2, tumv - sd3, tumv - sd4, tumv - sd5 and tumv - sd6 were respectively acquired from infected chinese cabbages and radishes in 3 cities ( taian, yantai and zaozhuang ) of shandong province

    利用rt - pcr方法克隆了tumv山東分離物的外殼蛋白（ cp ）基因，測定了它們的核苷酸序列、並將其與已報道的序列進行同源性比較和分析，最終確定了其歸屬地位，具體研究結果如下： 1 .從山東省3地市感病的白菜和蘿卜上分離到蕪菁花葉病毒的6個分離物，分別命名為tumv - sd1 、 tumv - sd2 、 tumv - sd3 、 tumv - sd4 、 tumv - sd5和tumv - sd6 。
  3. The percentage of polymorphic sites, degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method. the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified. these sequences, together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics

    汝us價ay1830 ）各12個個體進行rapd分析，對比多態位點比例、遺傳多態度以及遺傳距離，並構建neighbor - join噸系統樹;通過pcr擴增出線粒體165rrna基因，純化后直接測序，利用生物信息學方法進行序列分析和核昔酸變異比較，結合ge紅bar止中大西洋叉尾帶魚（ lepid （護腳caud玫tuseuphrasen1788 ）同源序列構建u甲cm叭系統樹。
  4. In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level

    本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆，通過核酸序列的差異比較分析，研究了該基因在不同物種中的進化關系；同時，通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究，獲得如下研究結果： （ 1 ）通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。
  5. This review summarizes the progression in preparation and preprocessing technologies of biological specimens. it especially introduces the preprocessing technologies, including centrifugation, filtration, dielectrophoresis, immunomagnetic separation, nucleic acid extraction

    摘要介紹生物標本的制備與預處理技術的進展，其中，重點介紹生物標本的預處理技術，包括離心分離、過濾、兩相電泳、免疫磁性分離及核酸抽提方法。
  6. This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv

    為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。
  7. Nuclear energy - chemical separation and purification of uranium and plutonium in nitric acid solutions for isotopic and dilution analysis by solvent chromatography

    核能.用溶劑色譜法對同位素和稀釋度分析用的硝酸溶液中鈾和鈈的化學分離和提純
  8. According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat, we designed primers and got the structure gene successfully. by 3 ' - race method combined with nested pcr, the 3 ' - end nuclear acid sequence was also obtained ; in additon, for the 5 ' - end sequence, we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer, and till now, partial 5 ' - end sequence has been amplified as well

    本研究根據先前分離純化所得天然tb22kda蛋白經maldi - tof - ms （質譜法）測得的氨基酸序列和文獻報道的過敏蛋白核苷酸序列設計引物，擴增克隆了該過敏蛋白結構基因的編碼序列；根據測得的序列設計特異性引物，並利用3 』 - race方法結合巢式pcr擴增得到基因的3 』末端；依據同源性比較的結果選用一段保守序列為5 』引物，並根據結構基因內部序列設計3 』特異性引物，進一步獲得了該基因5 』端的部分序列。
  9. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  10. An analytical method for simultaneous determination of anions in high - concentration phosphate solution in dayawan nuclear power plant by suppressed ion chromatography has been developed

    摘要建立了對核電站中含高濃度磷酸鹽水樣中常見痕量陰離子（氟離子、氯離子、甲酸根、乙酸根、硫酸根）梯度淋洗的分析方法。
  11. Utilizing ion - pair reversed - phase hplc, four kinds of oligonucleotides with size 18, 19, 20 and 21nt, respectively, were separated successfully. furthermore, two kinds of oligonucleotides with same size and difference sequence were successfully separated. linear regression equations of two standard samples have been set up

    在普通的高效液相色譜儀上，應用反相離子對高效液相色譜法成功地分離了4種長度不同的單鏈寡聚核昔酸（ 18 、 19 、 20 、 zlnt ）以及兩種序列不同的19nt單鏈寡聚核昔酸。
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