核酸分離 的英文怎麼說

中文拼音 [hésuānfēnlí]
核酸分離 英文
nucleic acid isolation
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  1. G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software

    本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。
  2. All data indicated that f i was a pure substance. third, using modern analytic techniques such as nucleic magnetic resonance ( nmr ), infrared spectroscopy ( ir ), ultraviolet visible absorption spectra ( uv ) and mass spectrometry ( ms ), we analyzed the basic chemical structure of f i and nominated it phthalic acid di ( 3 - methyl ) nanyl ester ( pdne ). we estimated primarily it was a novel natural compound

    第三，利用核磁共振，紅外光譜，紫外分析，質譜等現代分析測試技術對分離提取物f進行結構鑒定，解析出f的結構，初步推斷該物質為鄰苯二甲酸- （ 3 -甲基）壬酯（ pdne ） ，根據目前所掌握的資料，還沒有檢索到該物質相關的報道，初步認定為一種新型的天然產物。
  3. The entire earth may have been melted, and the iron and silicate components separated to form the core and mantle.

    整個地球被熔化，鐵和硅酸鹽分離而形成地核和地幔。
  4. Given the occurrence of split genes, it might be re - defined as the set of dna sequences ( exons ) that are required to produce a single polypeptide

    對分離的基因來說，它可被定義成一組需要用來產生蛋白質的脫氧核糖核酸序列，即外子。
  5. Nucleic acid isolation sublimate

    核酸分離純化
  6. When using the nuclear acid sequence and the predicted amino acid sequence of scghr to blast the fugu genome, we found that there were two contigs which shared the most similarity with scghr. therefore we realized there maybe were two ghrs encoded by different genes in teleosts. then, by means of nuclear acid sequence joining, orf of fghr1 and fghr2 were predicted from fugu genomic sequences using cohoghr isf1, cohoghr isf2, chghr and other related ghr amino acid sequence available at ncbi database

    當利用南方鯰ghr的核苷酸、氨基酸序列blast東方?的基因組時，發現有兩個contig與其氨基酸序列非常相似，因此意識到魚類可能存在兩種不同基因編碼的ghr 。隨後主要利用已經克隆得到的cohoghrisf1 、 cohoghrisf2 、 chghr以及其他魚類ghr核苷酸和氨基酸序列blast東方純的基因組，採用序列拼接的方法從東方?基因組中分離出兩種不同基因編碼的fghr1和fghr2 （本研究把和傳統ghr相似的叫做ghr1 ，把另外一種叫做ghr2 ） 。
  7. Sequence comparison indicated that the nucleotide homologies between the ha gene and those from eight other h3 subtype sfv isolates from china are over 95 %, and nucleotide identities between h3 sfv isolates in china and a typical h3 subtype avian influenza virus ( aiv ) strain dkuk1 - 63 are 95 % to 100 %. these might tell us that the ha gene of all h3 subtype sives isolated in china was originally from aiv although the vaccine based on ha do not protect against different subtype of influenza virus, it can provide favorable resistance to infection with antigenic variants within a subtype

    通過與其它7株國內siv分離株和6株genebank中的h3流感病毒ha序列比較分析，結果表明分離自我國不同地區的8株h3siv核苷酸序列同源性均在95 %以上，且與禽流感病毒（ aiv ）經典代表株dkuk - 63序列同源性在95 - 100 %之間，證實我國h3sivha基因來源於禽的流感病毒。
  8. Meanwhile, these six coat protein genes were sequenced and compared with other homologous sequences in genbank. the strain designation of the six isolates of tumv was finally determined. the results are as the following : 1. six isolates of turnip mosaic virus named tumv - sd1, tumv - sd2, tumv - sd3, tumv - sd4, tumv - sd5 and tumv - sd6 were respectively acquired from infected chinese cabbages and radishes in 3 cities ( taian, yantai and zaozhuang ) of shandong province

    利用rt - pcr方法克隆了tumv山東分離物的外殼蛋白（ cp ）基因，測定了它們的核苷酸序列、並將其與已報道的序列進行同源性比較和分析，最終確定了其歸屬地位，具體研究結果如下： 1 .從山東省3地市感病的白菜和蘿卜上分離到蕪菁花葉病毒的6個分離物，分別命名為tumv - sd1 、 tumv - sd2 、 tumv - sd3 、 tumv - sd4 、 tumv - sd5和tumv - sd6 。
  9. A chip based on electroelution principle was presented for the recovery of dna fragments, which can make the isolation and collection of target dna fragments possible in a single process by switching electrodes

    提出了一種基於電洗脫原理的核酸純化回收晶元，通過對晶元上電極進行適當的切換操作，可一次完成核酸樣品分離和純化回收。
  10. From dead chicken which infected infectious stunting syndrom of our province, one virue was isolated using spfeggs, chicken embryo fibroblast, mdck18, and vero cell. this virus was unable to agglutinate chicken erythrocytes. in order to definite the pathogeny of infectious stunting syndrom. physical and chemical specific property, types of the nucleic acid of the isolated virus, recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken

    為確定該病的病原，對所分離病毒進行了理化特性測定、病毒核酸型別測定、動物回歸試驗等生物學特性測定，證明該分離病毒與細小病毒科（ parvovirdae ）細小病毒屬（ parvovirus ）的雞細小病毒（ chickenparvovirus ）特性基本相符，核酸型為dna型。
  11. In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level

    本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆，通過核酸序列的差異比較分析，研究了該基因在不同物種中的進化關系；同時，通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究，獲得如下研究結果： （ 1 ）通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。
  12. Nuclear energy - measurement of radioactivity in the environment - water - part 1 : measurement of strontium 90 activity in water - radiochemical separation of strontium by nitric acid and measurement of yttrium 90 beta activity

    核能.環境中放射性的測量.水.第1部分:水中鍶90放射性測定.用硝酸對鍶進行放射化學分離和釔90放射性的測量
  13. Then the effect of turning channel has been studied. 7. this detection system has been used to dna segments separation experiments ; the results showed that it has met the need for dna separation

    將該微通道電泳晶元系統應用於不同熒光標記寡核苦酸、 dna片段的分離分析，實現了寡核著酸的單堿基分辨，同時應用於結核桿菌基因組pcr產物的分析，實驗結果表明此系統可以實現dna片段的快速高效分析。
  14. The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous, but lowly homologous with other reference strains. the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains, and is identical with the facts in the field cases. all the guangxi isolates are classified into genotype vii of apmv - 1, the same genotype dominated in china and other areas in recent years

    結果發現，廣西分離株之間在信號肚的核旮酸和氨基酸同源性很高，而與其它參考株差異較大；廣西分離株在裂解位點的氨基酸組成和排列均符合強毒株的特徵，並與毒株在臨床上的致病情況相符；根據apmvlf基因第47位第420位核苦酸序列所繪制的系譜樹吵ylogenetictree ）來看，廠西雞和鵝分離株都歸屬于基因型vll 。
  15. Studies of morin - al - nucleic acids ternary system and its analytical application

    桑色素-鋁離子-核酸三元體系的研究及其分析應用
  16. The room for the following equipments need the air - conditioning : uv - spectrophotometer, fluorescence spectrometer, gc, gc - ms, aas, afs, icp, icp - ms, hplc, hplc - ms, ion chromatography, atomic fluores - cence spectrometer, pcr amplification and balance

    12紫外/可見分光光度儀、熒光分光光度，氣相色譜，氣相色譜質譜、原子吸收，原子熒光儀、等離子發射光譜、等離子質譜、高效液相、液相色譜質譜、離子色譜、熒光光度、 pcr核酸擴增儀以及天平室安裝空調。
  17. In the experiment, tick - borne encephalitis virus ( tbev ) mdj - 01 strain isolated in mudanjing in 2001 was identified. and the biological characters and molecular biological characters were studied on, with senzhang strain isolated in 1953 as a control

    本試驗對2001年自牡丹江分離的森林腦炎病毒（ tbev ） mdj - 01株進行了鑒定以及生物學性狀和核苷酸全序列的測定的研究。整個試驗以1953年分離的tbev森張株為對照。
  18. The recombinant plasmid was identit1ed with restriction endonuclease, pcr, then sequenced. the resuit of sequence analysis showed that the vp3 gene is 1605bp and inc1uds a complete open reading frame encoding a protein of 534 amino acids. the hl isolate shares 98. 5 % and 98. 3 % identity with b isolate at nucieotide and amino acid levels respectively

    結果表明：鵝細小病毒h1分離株vp3基因全長1605bp ，編碼534個氨基酸，只有一個完整的開放閱讀框架，與國外已發表的鵝細小病毒b株核苷酸序列同源性為98 . 5 ，氨基酸序列同源性為98 . 3 ，表明這二個毒株親緣關系相近。
  19. This review summarizes the progression in preparation and preprocessing technologies of biological specimens. it especially introduces the preprocessing technologies, including centrifugation, filtration, dielectrophoresis, immunomagnetic separation, nucleic acid extraction

    摘要介紹生物標本的制備與預處理技術的進展，其中，重點介紹生物標本的預處理技術，包括離心分離、過濾、兩相電泳、免疫磁性分離及核酸抽提方法。
  20. Several kinds of preparation methods and the applied developments of magnetic polymer microsphere in cell separation, immobilized enzyme, dna separation and medicine carrier are discussed in detail, and the prospects of magnetic polymer microsphere are also proposed

    在此基礎上，對磁性高分子微球在細胞分離、固定化酶、靶向藥物、核酸分離等領域的最新應用及存在的問題進行了分析，並指出了該領域今後的研究方向。
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