核酸序列分析 的英文怎麼說

中文拼音 [hésuānxùlièfēnxī]
核酸序列分析 英文
nucleic acid sequence analysis
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  1. It ' s meaningful to study the function of rab proteins in ciliates and further to explicit the mechanism of vesicular trafficking. the rob gene was amplified from macronuclear dna of euplotes octocarinatus. the size of gene was 783 bp long with an orf of 624 bp encoding eorabl protein and containing three in - frame tga codes

    本研究利用pcr技術從游仆蟲（ euplotesoctocarinatus ）大核dna中擴增出rab基因，並對該基因進行序列分析，該基因全長為783bp ，兩端為端粒序列，編碼框為624bp ，編碼207個氨基酸，開放讀框中有3個tga ，在此編碼半胱氨酸。
  2. The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide

    核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。
  3. Through the analysis of the nucleotide and ammo acid of the sequences about e2 gene, it express that the e2 gene includes about 1100bp which encodes about 370 amino acides

    通過對pbne2p e _ 2基因的進一步基因序列和核苷酸序列分析，結果表明， e _ 2基因包括約1110個堿基，編碼約370個氨基酸。
  4. Rt - pcr was used to amplify the cdna of the genome. these cdna fragments were cloned into the plasmid pucm - t. the result indicated that the seguence of the genome was obtained. the genome of aev - nh937 composed of 7055 nucleotides, potentially encodes a polyprotein of 2134 amino acids. the genome of aev - nh937 has 94. 3 % nucleotides identity with the calnek vaccine strain of aev

    把它們分段克隆在pucm - t載體上，經序列分析，獲得了aev - nh937毒株的基因組全序列及推導的氨基酸序列，基因組全長為7055個核苷酸，與calnek疫苗株具有94 . 3的同源性，編碼一個含2134個氨基酸的多聚蛋白。
  5. Development of laboratory sequence analysis software based on www and unix

    的核酸序列分析實用軟體的開發
  6. Fachb440 was higher than that in the others. alignment based on deduced amino acid sequences indicated that spirulina fachb440 was different from that in other three samples of arthrospira. nucleotide sequence similarity among the three strains of the genus of arthrospira ( 96. 5 - 99. 6 % ) was higher than that between arthrospira and spirulina ( 78. 1 - 78. 5 % )

    Maximaouqdsm ）采螺旋藻（ spirulinafachb440 ） rubisco大亞基基因（ rbcl ）部分片段並進行了序列測定與分析，結果表明：螺旋藻（ spirulinafachb440 ）的gc含量比其他的節旋藻品系高；氨基酸序列分析發現螺旋藻與其他節旋藻品系的差異較大；螺旋藻與節旋藻核著酸序列相似性約為78
  7. The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced

    首先以中肋骨條藻的rdna序列為設計種特異性引物的靶區域，共設計出7對適合rfq - pcr的引物（依次命名為primer1 （ f r ） primer7 （ f r ） ） ，並用常規pcr實驗初步證明其中有4對引物（ primer1 （ f r ） 、 primer2 （ f r ） 、 primer5 （ f r ） 、 primer6 （ f r ） ）可作為中肋骨條藻特異性引物的候選者；然後測定了以primer6 （ f r ）為引物的pcr產物的序列，序列分析表明，中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大，從中可設計出滿足rfq - pcr需要的taqman探針（命名為taqman6 ） ；進一步的核酸雜交實驗表明， taqman6隻與中肋骨條藻的pcr產物雜交，不與其他藻的pcr產物雜交。
  8. Was multiplied and the tk gene was cloned. the cloned tk gene was retrieved by proper restrictive hemodynamics. the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin. then, the specificity and sensitivity of tk gene probe were detected with dot blot hybridization. the sequence of tk gene of nm98a strain was analysed. the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99. 7 %

    本研究中首次對iltv - nm98a株的tk基因進行了克隆和序列分析，結果表明： iltv - nm98a株tk基因的核苷酸序列與已發表的iltvtk基因的核苷酸序列具有高度的同源性，兩者之間僅相差4個核苷酸，同源性高達99 . 7 ，從而證實了iltvtk基因是高度保守的，為iltvtk基因核酸探針的制備提供了有力的依據。
  9. The percentage of polymorphic sites, degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method. the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified. these sequences, together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics

    汝us價ay1830 ）各12個個體進行rapd分析，對比多態位點比例、遺傳多態度以及遺傳距離，並構建neighbor - join噸系統樹;通過pcr擴增出線粒體165rrna基因，純化后直接測序，利用生物信息學方法進行序列分析和核昔酸變異比較，結合ge紅bar止中大西洋叉尾帶魚（ lepid （護腳caud玫tuseuphrasen1788 ）同源序列構建u甲cm叭系統樹。
  10. In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level

    本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆，通過核酸序列的差異比較分析，研究了該基因在不同物種中的進化關系；同時，通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究，獲得如下研究結果： （ 1 ）通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。
  11. In comparison with genbank data, the homologies of the nucleotide sequence and amino acid sequence were as following : hc was 98. 4 % and 100 % ; ha was 97. 2 % and 99. 3 % respectively. 4. two recombinant prokaryotic expression vector were constructed which has complete open reading occlusing initation codon, leader signal peptide sequence and termination codon

    序列分析表明，所克隆獲得的基因與genbank中已經登錄的核苷酸和氨基酸的同源性分別為： hc98 . 4和100 ， ha97 . 2和99 . 3 ，證明本試驗蟲株與國外報道的同源性很高。
  12. Hovever, the gene encoding the mature papain peptide was amplified using pcr from genomic dna extracted mid - development carica papaya fruit. about 98. 8 % of cdna nucleotide sequence reported in literature were homologous to the responding sequences of our study. there are three introns in the gene, in which the content of a and t is 86. 0 %, 79. 5 % and 90. 6 % respectively

    同時本研究以木瓜基因組dna為模板，通過pcr反應獲得了編碼木瓜蛋白酶成熟多肽部分的核苷酸序列，序列分析表明該基因含有三個內含子，其長度分別為157bp 、 266bp 、 88bp ， at含量分別為86 . 0 ， 79 . 5 、 90 . 6 。
  13. Using persistent changing probability for analysis of nucleotide sequences

    分析核酸序列的持變概率方法
  14. Supply of concentrate haemodialysis solution with bicarbonate powder buffer to the hospital authority and the department of health as a 24 - month contract from date of acceptance design, supply, delivery, installation, commissioning, maintenance of hardware, software and related services for the implementation of the automated tag and information display system for the immigration department on or before december 2006 supply of 320 000 kg. of polyelectrolyte type ii to the drainage services department as a 36 - month contract from date of acceptance provision of dental laboratory work for the department of health as a 24 - month contract from date of acceptance supply, installation and commissioning of a ground reception system for meteorological data from multi - functional transport satellite for the hong kong observatory from date of acceptance to fulfillment of contractual obligations supply and installation of 1 set of automated dna sequencing system to the department of health from date of acceptance to fulfillment of contractual obligations

    承投為醫院管理局和?生署供應高濃度血液滲析液連炭酸氫鹽緩沖粉劑，合約由發出接納書日期開始，為期24個月為入境事務處於2006年12月或之前推行自動化籌號及資訊顯示系統供應硬體和軟體，包括設計、送貨、安裝、試機、保養及有關服務為渠務署供應320000公斤高分子電解質（第ii類） ，合約由發出接納書日期開始，為期36個月為?生署提供牙科製品服務，合約由發出接納書日期開始，為期24個月為香港天文臺供應一套多用途輸送衛星氣象數據地面接收系統，包括安裝及試機服務，由發出接納書當日至履行合約訂明的責任為止為?生署供應和安裝一套核酸序列自動測定系統，由發出接納書當日至履行合約訂明的責任為止
  15. The dissertation consists of five chapters : in chapter one, the recent progress in molecular approaches in systematic studies of macroalgae e. g. dna extraction, restriction endonuclease fragment length polymorphisms ( rflps ), random amplified polymorphic dna ( rapd ), gene sequencing, intersimple sequence jepeats ( issr ), amplified fragment length polymorphisms ( aflp ) and single strand. conformation polymorphisms ( sscp ) were reviewed

    本論文由五部分組成：在第一部分，綜述了大型海藻dna的提取、限制性片段長度多態性（ rflps ） 、隨機擴增多態性dna （ rapd ） 、核酸序列分析、擴增片段長度多態（ aflp ） 、單鏈構象多態（ sscp ）等分子手段在大型海藻系統學研究中應用的一些進展。
  16. In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail

    3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。
  17. Sequence analysis indicated that the opening reading frame of ns1 gene is 1056nt in length, encoding 352 amino acids, identical to the sequence of strain sa 14 - 14 - 2 deposited in genbank. ns1 gene in the pmd18 - t - ns1 was cut by bamhi and hindiii, was cloned into the expression plasmid pet - 30b ( + ) treated with the same enzymes, resulting in a recombinant plasmid pet - 30b - ns1. the pet - 30b - ns1 was identified by pcr, restriction digestion, and sequencing

    通過序列分析結果表明， jev - ns1基因編碼區核苷酸序列長度為1056bp ，編碼352個氨基酸，序列分析和比較表明本實驗克隆到的ns1蛋白基因與genbank中收錄的疫苗株sa14 - 14 - 2的核苷酸序列一致，表明盡管該疫苗株在我國應用多年，但ns1基因並未發生變異，提示該疫苗株遺傳性狀比較穩定，是一株優良的疫苗株。
  18. The result demonstrates that the sequence of the coding region is 1167bp, encodes a protein of 389 amino acid

    2kb左右的片段。核酸序列分析表明，該片段的編碼區長1167bp ，編碼389個氨基酸。
  19. Sequence analysis the cdna clone contains an 546bp orf ( open reading fragment ) encoding a predicted protein of 181 amino acids with molecular weight of 20. 7kd, 130bp 5 ' non - coding region and 152bp 3 " non - coding region including polyadenylation signal sequence aattaa and poly a tail. 3

    棉花arf基因的序列分析核苷酸序列分析表明， gharf的全長為828bp ，其中含有一個從131bp到676bp間的546bp構成的一個完整開放閱讀框，它編碼181個氨基酸、分子量為20 . 7kd的蛋白質。
  20. Molecular epidemiology of avian infectious bronchitis viruses isolated in guangxi by pcr and dna sequencing technique

    及核苷酸序列分析技術對廣西雞傳染性支氣管炎病毒進行分子流行病學研究
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