沉澱抗體 的英文怎麼說
中文拼音 [chéndiànkàngtǐ]
沉澱抗體
英文
precipitating antibody-
The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated
Niptg誘導表達，產物沉澱中於約45kda處顯見高合蛋白表達帶， westernblot分析表明，該蛋白帶可被篩庫血清中特異性lge抗體識別；而載體本身表達的26gst蛋白帶則否。However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting
利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。This study demonstrated that the arabidopsis f - box protein coil associated with atcul1, atrbxl and skpl - like proteins askl and ask2 to assemble scfcoil ubiquitin ligase complexes. also, we found that the atcull component of scfcoil complexes contained two species including atcull and modified atcull. ( 2 ) we found that coil assembled to two separate scfcoil complexes with either askl or ask2 through immunoprecipitation analysis with plant expressing myc - tagged version of ask2
用表達融合蛋白myc - ask2的擬南芥為材料，以- myc抗體進行免疫共沉澱分析發現， myc - ask2蛋白可以與coi1蛋白一起免疫共沉澱，但是不能與ask1蛋白免疫共沉澱，表明coi1蛋白與ask2蛋白，但是不能同時與ask1結合形成scf ~ （ coi1 ）復合體。The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr. it seems that the increased 1, 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n, while the decrease of this branch may prevent these processes
用特異性抗體結合westemblot結果發現，正義或反義gnt一vcdna的轉染並不引起pkb 、 p44 / 42mapk和mek蛋白質表達的變化，而gntv一s / h 」 21細胞pkbt308 、 5473位點磷酸化和免疫沉澱pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化為指標的pkb的活性都較mock細胞增加， gntv一as / h7721細胞中這些指標的變化則相反。Ghg - electrical grade activated silica powder is produced by way of mingling on the basis of electrical grade silica powder. it has activt effect on surface & make silica powder mingled with resin, raise cohesive force between resin & silica powder, raise the water - resestance on the sunface and the compressive strength of pour object, reduce sediment, gradation & split, increase filler of silica powder, and replaced inpoted products on the producing line of imported dry type transfomer & high tension mutual induc tance equiment, it has been the best pouring insulate materual in electrical trade
電工級活性硅微粉是在電工級硅微粉的基礎上進行偶聯化處理而製成，具有表面活性作用，能使用硅微粉與樹脂發生交聯，提高樹脂與硅微粉的粘結力和界面增水性，提高澆注體抗沖擊強度，減少沉澱、分層、開裂現象，增加硅微粉的填充量，在引進的乾式變壓器、高壓互感器生產線上已成功地代替了進口產品，成為電工行業理想的環氧澆注絕緣材料。The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male
Page電泳結果表明：麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白，分子量分別為181kd和136kd ； sds - page電泳分析：存在3條雌特異性帶，其分子量為123kd 、 120kd和91kd ，由此，可推定卵黃原蛋白（ vitellogenin ， vg ）和卵黃磷蛋白（ vitellin ， vn ）由2個蛋白組成，其中分子量較大的蛋白由2個亞基組成；雙向電泳結果顯示，在120kd附近有兩個特異性點，其等電點為5 . 5和5 . 7 ；雙擴散表明，麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應，而與雄蜂血淋巴無免疫反應，說明了vg與vn具有免疫同源性，是雌特異性蛋白，且由脂肪體合成。The main work is introcuced as follows : ( 1 ) the inorganic - organic nanocomposite of nanogold and chitosan, which have high biocompatibility, was synthesized in situ and used for the immobilization of antibodies. an amplified piezoelectric immunosensor for detection of normal human igg was designed with this nanocomposite film. comparing with self - assembling film of semi - cystamine, the inorganic - organic nanocomposite film could immobilize more antibodies with the activity of antibodis well retained
與半胱胺自組裝膜比較，納米金-殼聚糖復合物膜較大的比表面積使其可固載更多的抗體，同時可較好地保持抗體的反應活性;納米金的物理吸附作用使更多的沉澱結合於石英晶體表面，以其為載體制備的傳感器具有更大的頻率響應值。A method for preparation of antithrombin ( at - ) concentrated from human plasma ' s fraction has been described. after preliminary treatment of plasma with the cold ethanol, the isolation of at - iii from the precipitate was performed by affinity chromatography on heparin - sepharose cl - 6b ; desalted by dialysis and concentrated
從人體血漿中提取較高純度的抗凝血酶（ at - ）的，通過低溫乙醇處理血漿，得到的沉澱經肝素-瓊脂糖兩次親和層析，並透析、濃縮獲得at -的初品。( 3 ) we further demonstrated that the atcull component of scfco11 complexes was modified via axr1. mutations axr1 resulted in reduction of the modified atcull component of scfcol1 complex. ( 4 ) the mutant alleles of axri ( axr ] - 3 and axr ] - 12 ) showed moderate insensitivity - to ja - inhibitory root growth and mild reduction of ja - inducible gene expression of atvsp, thi2. i and pdfi. 2 compared with that of wild type
以表達融合蛋白flag - coi1的axr1基因突變體axr1 - 3為材料，用- flag抗體進行免疫共沉澱分析發現， scf ~ （ coi1 ）復合體中修飾的atcul1蛋白的含量明顯降低，表明axr1基因突變影響對scf ~ （ coi1 ）復合體中修飾的atcul1蛋白的修飾。The scfco, " ubiquitin - ligase complexes are required for jasmonate response in arabidopsis ( l ) arabidopsis coi is required for all the jasmonate - regulated response. it encodes a protein containing leucine - rich repeats and a degenerate f - box motif these structural features are characteristic of f - box proteins that function in ubiquitin ligase complexes for the ubiquitylation of substrate - proteins targeted for degradat1on
以表達融合蛋白flag - coi1的擬南芥為材料，用- flag抗體進行免疫共沉澱分析發現， coi1蛋白可以與ask1 、 ask2 、 atrbx1和atcul1蛋白結合形成scf ~ （ coi1 ）復合體，並發現其中的atcul1蛋白包括未修飾和修飾的兩種形式。( 3 ) the piezoelectric quartz crystal impedance ( qci ) technique was employed to monitor in situ the pb2 + - induced precipitation of bsa onto a gold electrode and the precipitate dissolution via edta in an aqueous solution
（ 3 ）用壓電石英晶體阻抗法（ pqci ）現場監測了水溶液中鉛離子誘導牛血清白蛋白（ bsa ）沉澱及沉澱由na _ 2edta溶解的過程。The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography. the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b. after washing the unspecific proteins, change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively
方法是先用硫酸綏鹽析，再過親和層析柱，即33飽和硫酸按沉澱后，透祈過夜，上p24抗原做配體的濱化氰活化的sepharose4b柱，流洗掉雜蛋白和抗人紅細胞單特異性抗體后，再洗脫並收集雙特異性抗體。Methods 1 mr1 hybridoma was cultured in hollow fibre system. culture supernatant was harvested, then precipitated by half - saturated ammonia sulphate. mfm was purified by deae - sephacel ion exchange chromatography. mri concentration was measured by elisa
硫酸錢半飽和沉澱， oeae陰離子交換層析分離純化抗co40l單克隆抗體mri 。 pbs透析后， 505一隊ge檢測純化抗體的純度， eusa測定抗體濃度。Because the limited concentration that macromolecular materials absorb liquid antistatic reagent is very low, it is better if add appropriate solid powder material when preparing high - concentration antistatic master batch. there are a little precipitation in product, but no effect on use effect
由於高分子材料吸收液體抗靜電劑的極限濃度較低，製作高濃度抗靜電母料時，可加入合適的固體粉末材料。本品有少許沉澱物，不影響使用效果；使用時最好先用乾燥、潔凈的棍棒適當攪拌，以提高本品的均勻性。分享友人