活性片段 的英文怎麼說

中文拼音 [huóxìngpiānduàn]
活性片段 英文
avtive fragment
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 片 : 片構詞成分。
  • 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
  • 活性 : [化學] activity; active; activated活性肥料 active fertilizer; 活性酵母 active dry yeast; 活性粘土...
  • 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
  1. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  2. Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank

    以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。
  3. Five analogues and five segments were designed and synthesized by using solid phase synthesis method according to separated papaver somniferum pollen tridecapeptide with antitumor activities as leading peptide. their primary secondary structures in solution were determined by cd spectra and their inhibitive activities to human liver and mammary gland cancer cells were assayed by mtt method. the relationship of structure - activity was studied and discussed

    罌粟花粉十三肽對人肝癌和人乳腺癌腫瘤細胞具有明顯的抑制作用，以其為先導化合物，設計併合成了5個類似物和5個片段，結合cd譜測定的二級結構及它們對人肝癌和人乳腺癌腫瘤細胞的抑制作用測定結果，研究並討論了該肽結構與抗腫瘤活性的關系
  4. In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated

    將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活，導致發酵產物中4個a組分（不需要的組分）的消失；將基因簇中的avec基因通過同樣手段，使其失活，導致發酵產物中4個「 1 」組分的消失，而主要積累「 2 」組分（進一步改造可成為伊維菌素的前體b _ 2組分） 。
  5. Thirdly, the hyl gene was cloned to puc19 and pbr322 respectively. then the fragment containing ampr derived from puc19 and hasa came from pse380 - has were inserted into hyl either or both in vitro. transformed the recombinated vectors into s. equi by electro - operation, then plating on hag solid medium containing ampicillin, and selected clones hi which the hyl gene was inactivated by gene replacement through homologous recombination

    （ 1 ）直接插入外源基因滅活hyl方法：將hyl片段與載體puc19 pbr322連接后，用來自puc19的amp ~ r抗性基因片段從hyl基因的中部插入將載體上的hyl片段分為兩部分； （ 2 ）用hasa替換hyl部分片段的方法：將hyl片段與載體pucl9 pbr322連接后，用hasa片段替換部分hyl ，再將amp ~ r片段接入到hasa上游。
  6. These pieces of dna were found to contain genetic information that might play a role in the survival and infectiousness of this pathogen

    這些dna片段包含的遺傳信息可能與該病原體的存活和感染性有關。
  7. The hk dvd version is a two disc package, with disc one featuring the film two endings are included but the alternate ending destroys the consistency of the movie a lot and is not recommended ! and disc two containing all the bonus materials. easter egg can be found on disc 2 by tapping down the cursor until the english film title

    無間道的dvd是雙碟裝，碟一是本片包括兩個結局，但大陸版的結局草草收場，而且破壞了全片劇情的統一性，不值一看，碟二是特別收錄，有製作特輯花絮music video等，另外還有隱藏的復活蛋收錄，只需在目錄不停向下按，直到指標移到左邊的infernal affairs英文片名，按下確定便會發現有八分鐘的ng片段。
  8. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  9. The gene of amoa in ammonia - oxidizing encodes the active - site polypeptide of ammonia monooxygenase which catalyzes the oxidation of ammonia to hydroxylamine. we designed a pair of primers special for the amoa gene by comparing the known amoa gene sequences and used pcr to amplify the amoa gene fragments

    Amoa基因是編碼氨單加氧酶活性多肽位點基因，我們通過引物篩選合成了對氨氧化細菌amoa基因特異結合的引物序列，利用pcr技術對活性污泥中的amoa基因片段進行特異擴增，得到的dna片段大約為490bp 。
  10. The blast analysis of the 3. 0kb segment indicate that it is similar to translational activator and has more than 80 % homology to two genes in arabidopsis and oryza saliva respectively

    將所得約2 . 8kb的基因片段作blast分析，表明其可能屬于翻譯激活子基因家族，並在擬南芥和水稻中皆有同源性大於80的同源基因。
  11. This result show the deleted fragment is n ' t the key domain to transactivation. 2 ) the mutant p6p2 translate a peptide includiing 165 amino acid and acted as inhibition to sv40 promote

    （ 63加152 1079氨基酸段）對sv40啟動子的均有轉錄激活活性，這表明缺失的氨基酸片段（ 64 151氨基酸序列）對轉錄激活是非必需的。
  12. There is no characteristic in the amino acid sequence 63 - 152 and it is the piece that we want to delete to identify the function of the segment. ie180 gene mutants deleted the 64 - 151 amino acid was amplified by muti - pcr and were cloned by pmdist - vector. the clone plasmids were named pjmp1p3p2. the segment corresponding to the sequence of 1 - 1079 amino acid of the genbank sequence amplified by pcr, its clone plasmids was named pjmp1p2. the segment corresponding to the sequence of 454 - 1079 of genbank sequence amplified by pcr and its clone plasmids is named pjmp6p2 - three clone plasmids and pcdna3 were digested by restriction enzyme bamhi and hind, the gene segment of p1p2, p1p3p2, p6p2 were recycled

    本試驗應用dnamis及prosis軟體分別對genbank中登記號為no352564的ie180序列進行了蛋白質序列分析，結果表明其1 - 34段氨基酸序列具有典型helix - turn - helix特徵序列，並且富含酸性氨基酸d及e ，是典型的dna識別序列；富含絲氨酸序列的152 - 409氨基酸序列是一個與激活有關的、潛在的磷酸化位點； 454 - 696氨基酸區域是dna結合域； 64 - 151氨基酸片段沒有明顯的序列特徵；從中可看出ie180蛋白的1 - 1080氨基酸段具有典型的轉錄激活因子結構特徵。
  13. The full - length sequence, the 3 ' - deletion fragment, the sequence encoding the mature protein and the sequence encoding the conservative domain, were cloned using synthesized primers. recombinant expression vectors were constructed through directional cloning and then host e. coli were transformed by the vectors

    設計特異引物克隆得到毒蛋白基因的4個片段，即基因全長、末端缺失片段、編碼成熟肽的片段及編碼活性區域的片段。
  14. The former expresses recombinant proteins with a 6xhis tag at n - terminal. the fore mentioned four fragments were all used for expression in this system and the mature protein and the conservative domain were effectively expressed while the expression of the other two was unobvious

    前者表達n ?末端加6 his標記的融合蛋白，克隆到的4個基因片段均進行了表達，其中成熟肽和活性區域得到了大量表達，蛋白全長和末端缺失片段表達不明顯。
  15. A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained

    將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。
  16. A new e. coli promoter - probe vector phn1005 was constructed by using a red - shift enhanced gfpmut3 as reporter gene and showed the following characters : 1, bamhi at the 5 " end of gfpmut3 structure gene could be used to clone promoter - active fragment and the strength of promoter could be quantitatively assayed. 2, a rrnbtlt2 terminator from rrna gene at the 3 " end of gfpmut3 could permit clone strong promoter. 3, another rrnbt1t2 terminator was inserted upstream bamhi to prevent reading through of promoters from puc19

    進一步以紅移且熒光強度提高21倍的gfpmut3為報告基因，構建了大腸桿菌啟動子探針載體phn1005 ，該載體上gfpmut3結構基因5 』端的bamhi位點可用來克隆具有啟動子活性的dna片段並定量分析插入的啟動子強度；其3 』端含rrnat1t2終止子，可允許克隆強啟動子；在bamhi上游同樣插入rrnat1t2終止子以防止載體puc19上的啟動子的轉錄通讀； gfpmut3結構基因上游還插入一段內含子序列使正反六種讀碼框的翻譯均可被終止，可保證gfpmut3以正確的讀碼框翻譯。
  17. Among sequenced 16 positive clones randomly selected, two represent novel expression tag sequences, two are homologous to two unknown proteins in genbank ; the rest are homologous to known or putative proteins or enzymes with definite functions by searching the genbank through blast program, which are involved in various life activities of cell such as regulation of gene expression, plant secondary metabolism, signal transduction, adversity resistance, stress response and defense reaction. significant changes of quantities of these gene fragments were observed before and after ssh, which indicated they were enriched after ssh

    隨機挑選了16個差異表達的克隆進行序列測定，經與genbank數據庫相關數據比較分析，發現有2個新的cdna片段（ ets ） ，有12序列與genbank中已知蛋白或推測蛋白質（ putativeprotein ）序列有高低不同的同源性，它們參與基因的表達調控、植物的次生代謝、信號傳導、抗逆、應激及防禦反應等細胞生命活動過程。
  18. A fusion - trascriptional library was then constructed by inserting sau3ai - partially digested chromosomal dna from 7653r into bamhi cloning site of phn127. a group of constitutive - expression fusions with different fluorescent strength were obtained

    將7653r的總dna經sau3ai部分酶解並克隆到phn127上，獲得的融合子庫，從中篩選到gfp組成型表達的具有調控活性的dna片段。
  19. ( 4 ) in artificial pepsin digestive system with ph2. 0, due to the acid denaturation, the pigg was hydrolyzed wholly, in the system with ph3. 0 and 4. 0, although not all entirely pigg could be detected, but the pigg antibody activities against e. coli still existed without changing, this indicates that the f ( ab " ) 2 with antigen - binding activity still remain complete during digesting process

    （ 4 ）在ph2 . 0的人工胃液消化體系中，由於酸變性pigg全部水解；在ph3 . 0和ph4 . 0的人工胃液消化體系中，盡管檢測不到所有的pigg ，但pigg仍可與e . coli特異性結合且凝集價未發生變化，表明在消化過程中具有抗原結合活性的f （ ab ） _ 2片段仍可完整地保留下來。
  20. The study of in - situ construction of cytocompatible surface on pdl - la matrix via amphiphile - amino acid ( rgd ) hybrid self - segregation - the amphiphilic diblock copolymer, poly ( dl - lactide ) - poly ( ethylene oxide ) ( pla - peo ) copolymer, containing hydrophobic pla block and hydrophilic peo block was synthesized via coupling method in this dissertation. cell - adhesion - promoting amino acids and integrin receptor peptide rgd were then immobilized at the end of peo chain of pla - peo copolymer via hydroxyl group activation technique. the solvent blending and casting method was then used to obtain the amphiphile modified pdl - la membranes

    兩親共聚物-氨基酸（ rgd ）雜化體原位自修飾構建聚乳酸細胞相容性表面的研究一本論文首先設計併合成了一類含疏水聚乳酸（ pla ）鏈段和親水聚氧乙烯（ peo ）鏈段的兩親嵌段共聚物材料（ pla - peo ） ，利用peo鏈端的活性官能團羥基固定了促細胞粘附的氨基酸及整合素配體多肽片段rgd 。
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