活性發酵 的英文怎麼說

中文拼音 [huóxìngfājiào]
活性發酵 英文
active fermentation
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 發 : 名詞（頭發） hair
  • 酵 : 動詞（發酵） ferment; leaven
  • 活性 : [化學] activity; active; activated活性肥料 active fertilizer; 活性酵母 active dry yeast; 活性粘土...
  1. In the research field of food preservation, an intense interest for scientists is focused on the isolation of antimicrobial substances from microbial metabolites. natural biopreservatives derived from traditional fermented foods are generally recognized as safe. in this paper, one strain screened for its safety and broad antimicrobial spectrum and its antimicrobial substance - - bacteriocin are studied in detail

    本文從傳統的發酵食品中篩選出一株安全、具有廣譜抗菌活性的芽孢桿菌，並對其抗菌活性物質細菌素進行分析，為微生物天然生物防腐劑的開發以及在食品中的應用提供理論依據。
  2. Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted

    因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明， arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝： （ 1 ） arg可能影響胞外蛋白酶的活性，進而促進含氮大分子物質的分解代謝，補充發酵過程中的氮素來源。
  3. The cultural conditions such as temperature, fermentation period and the compound of medium are studied. the result of test show that suitable factors for both bacterium to grow and active substance to produce are 28, 200rpm and 72 hours. the bacterium is gotten through centrifuge with 8000rpm for 20min. then the bacteria is diluted and colophony named s - 8 is put into and used to absorbed active substance for 4 hours

    對該菌株發酵條件的研究表明：該菌株用馬鈴薯葡萄糖液體培養基發酵培養， 28 ， 200rpm搖瓶中振蕩培養72h可獲得高活性的發酵產物，用蘇雲金芽孢桿菌hd - 1做指示菌，將發酵液稀釋40倍生測仍可形成明顯的抑菌圈。
  4. The metabolites eliciting inhibition to foam cell formation process of macrophage produced by endophyte hccb00017 were studied. several products were isolated through solvent extraction, and silica gel chromatography et al. one compound, hccb00017 - a, showed cytotoxicity ; the other two, hccb00017 - c and hccb00017 - e, showed inhibitory activity against foam cell formation process of macrophage

    對具有巨噬細胞泡沫化抑制活性的植物內生菌hccb00017的代謝產物進行研究，應用溶媒萃取、硅膠柱分離等方法，從其發酵液中分離出具有細胞毒性的活性物質hccb00017 - a ，以及具有巨噬細胞泡沫化抑制活性的組分hccb00017 - c和hccb00017 - e 。
  5. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。
  6. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  7. Conclusion : the system not only could pick upon the op timum strain on fixed culture medium or the optimum substrate to special strain fleetly but also could be used to evaluate the microbe growth character apace, monitor z ymogen upgrowth in fermentor and determine total number of bacteria in fresh cre amery rapidly

    結論：研製的生物傳感器系統可用於菌種和培養基組分的快速篩選、微生物活細胞生長特性的快速評估，還可用於發酵罐中發酵菌的生長監測及新鮮牛奶中雜菌總數的快速測定。
  8. In order to increase the number of living bacterium, the proper alkali or alkali salt was added to ferment liquid to eliminate stressing effect of organic acid on lactobacillus acidophilus

    摘要採用向發酵液中添加適當的堿或堿性鹽等物質的方法，消除了影響嗜酸乳桿菌生長的有機酸的用，達到單位體積的發酵液內含有更多活菌體的目的。
  9. Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b, in which the yields of avermectins b in 82 isolates were only about 0. 1 ~ 2 % of those produced by the parental strain olm73 - 12. 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis. the mutant only producing avermectins bl was not detected

    搖瓶發酵和hplc分析結果表明，有99個突變株仍然產阿維菌素b的4個組分，其中82株的發酵單位很低，僅為出發菌株olm73 - 12的0 . 1 2 ，從中挑取2株經pcr擴增和測序驗證，均發生了正確的基因取代；沒有檢測到僅產b1的突變株，這表明阿維菌素b2組分的產生並不是因為阿維菌素pks上dh2的部分活性所造成。
  10. In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated

    將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活，導致發酵產物中4個a組分（不需要的組分）的消失；將基因簇中的avec基因通過同樣手段，使其失活，導致發酵產物中4個「 1 」組分的消失，而主要積累「 2 」組分（進一步改造可成為伊維菌素的前體b _ 2組分） 。
  11. Besides, the endo - cellular metabolites of the all strains were detected and screening, based on the analysis methods in which samples were compared with authentic ginkgolides by hplc and esi - ms. thus, it may be concluded that ginkgolide a, ginkgolide b and bilobalide occur in metabolites of strains f3 and g3b

    在菌株f _ （ 1 - 3 ）的發酵液中，檢測到有抗革蘭陽性菌的物質存在，通過對活性成分的追蹤，分離得到化合物f1 - 3a和f1 - 3b 。
  12. A review surveys the application of surfactants in fermentation industry. application of antifoamer, disinfection and antiseptic, bioseparation are introduced in detail

    摘要綜述了表面活性劑在發酵工業中的應用，具體介紹了在消泡、滅菌消毒、分離三個方面的應用。
  13. The study was performed to isolate biosurfactant - producing bacteria from composting at different temperature, 45 and 55, biosurfactant ' s producing was determined by measuring the surface tension of fermentation

    從不同溫度的堆肥過程中（ 45和55 ）篩選產生物表面活性劑的細菌，生物表面活性劑的產生通過測定其發酵液的張力值來判斷。
  14. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  15. After ten generations growing on drug - free plate, the resistance of pa9911 - c64 kept unchanged. 800 broth samples of endophytes were tested on this model, among which several samples showed antimicrobial or synergic activity

    以此耐藥突變菌株作為模型，對800份植物內生菌所產生的發酵樣品進行了初步篩選，並獲得了幾份活性樣品。
  16. 1760 samples produced by endophytes reserved by shanghai health creation center for biopharmaceutics r & d were tested on several models, and many bioactive samples were selected. the result of second round indepth - screening for these bioactive samples demonstrated that three samples still possecced great inhibitory ability against foam cell formation process of macrophage

    本研究對1760份植物內生菌發酵樣品的活性通過多個模型進行了初步篩選，結果發現多個活性樣品，對活性樣品進行復篩，發現其中有三份樣品對巨噬細胞泡沫化抑制活性仍較強。
  17. It is studied that the activity against eumycetes of the fermentation filtrate ( 16h ) of strain jw - 725 is the strongest. the optimum nutritional sources are wheat bran and soya bean meal. the fermentation filtrate reserved under 4 still has bioactivity after 20 days or so, but its bioactivity decreases much while being treated under high temperature. the ph of fermentation filtrate has much influence upon the bioactive substance, and it remains activity at the range of 5

    發酵液在4下保存20天左右仍有抗菌活性；但經高溫處理后，其抗菌活性降低很多； ph對抑菌物質的生物活性影響比較大，抑菌物質保持活性的ph范圍是5 . 0 7 . 0 。分離到的多粘芽孢桿菌（ b polymyxajw - 725 ）對柑橘青黴菌（ p
  18. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  19. Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia

    挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后，用釩鋁酸按法測定了表達產物的酶活性，結果表明重組菌株可有效表達具有生物學活性的植酸酶。
  20. The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively

    三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
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