活細胞培養法 的英文怎麼說

中文拼音 [huóxìbāopéiyǎngfǎ]
活細胞培養法 英文
biocytoculture
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52

    以細胞培養液m199 （含20 %的小牛血清）為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，採取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍，成活率為72 % ，第二組冷凍保存13天後解凍，成活率為520 / 0 。
  2. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  3. To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0. 25 % trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by mtt method, the viable count was detected by the rejection experiment of trypanblau

    摘要為探討適用於黃牛輸卵管單層上皮細胞的培養方法，採用機械剪取及0 . 25 %胰酶消化的方法分離獲得上皮細胞，取對數生長期細胞進行mtt比色實驗檢測細胞活力和生長速度；臺盼藍排斥試驗檢測活細胞數。
  4. In vitro endothelial proliferation inhibiting activity of recombinant angiostatin was examined with mtt method by using human umbilical vein endothelial cells ( huvec ). in this test we could draw the inhibition curve and calculated the ic50 to confirm its bio - activity further we do cam vascular inhibition test in vivo

    4驗證重組蛋白angiostatin的生物活性：採用mm法測定重組蛋白對原代培養的人臍靜脈內皮細胞（ huvec ）的抑制作用，繪出抑制曲線，並計算出ic 。
  5. Methods for qualitative and quantitative characterization of primmorph culture were developed, which included a modified mtt assay for measurement of cell viability and cell growth, a 5 - brdu - incorporation assay for detection of cell proliferation, propagation and metabolism of in vitro primmorph

    系統考察並優化了mtt法應用於海綿細胞培養過程細胞活性和細胞生物量定量的條件，結合5 - brdu摻入法建立了細胞團培養過程中細胞增殖能力和細胞活性的評價方法。
  6. The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased

    結合以上創建的方法和免疫熒光流式細胞術，用k562細胞株可溶性分泌物（上清）對外周血單個核細胞（ pbmc ）進行培養以模擬體內微環境，然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究，結果發現用腫瘤上清培養的pbmc細胞數量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細胞數的下降主要是由細胞壞死和凋亡引起的，腫瘤上清對細胞周期沒有阻斷作用，反而略有促進作用； t細胞亞群比例增加，並促進表達th1 、 th2細胞因子。
  7. The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment. in brief, the intact blastocysts were plated on sto feeder layer treated with mitomycin, and were cultured in the media supplymented with brl condition medium

    聯合evans和martin的方法，稍加改良來分離小鼠胚胎幹細胞，把昆明白小鼠完整的囊胚直接種植在經絲裂黴素滅活的sto飼養層細胞上，在含有brl條件培養基的es細胞培養液中培養。
  8. During the culture of the porcine ear skin fibroblasts, the successful rate was increased by selecting culture methods, contrasting donor age and improving culture condition. the rate of living cells in the method of combining trypsin cold treatment with trypsin heat treatment was much higher than that in the method of trypsin heat treatment. the results showed that the method of combining trypsin cold treatment with trypsin heat treatment had less adverse effects on cells than the method of trypsin heat treatment

    在培養豬耳皮膚成纖維細胞時從篩選細胞分離方法、比較供體年齡和改善培養條件這三方面著手提高培養的成功率。實驗中發現胰蛋白酶冷熱處理結合法培養的細胞存活率明顯高於胰蛋白酶熱處理法，說明使用胰蛋白酶冷熱處理結合法對細胞的不利影響較少，細胞產量較高。
  9. Cell morphology was similar no matter what method was used. percentage of live cells was significantly higher in erythrocyte splitting method that was ( 91 4 ) % than in centrifugation method that was ( 83 5 ) %. proliferative ability of mscs seperated by erythrocyte splitting method at 2 to 6 generation was much higher than by another method. the maximal cell number was 4. 67 vs 4. 10 times that of the initial cell number at cell inoculation

    紅細胞裂解法較密度梯度離心法細胞貼壁較慢， 36小時才可見零星細胞貼壁，兩種方法細胞形態相似。活細胞百分比，紅細胞裂解法為（ gi士4 ） ，密度梯度離心治（ 83士5 ） ，有明顯差異。傳代培養： 2 6代范圍內，紅細胞裂解法較梯度離心法細胞增殖更旺盛（分別是按種時細胞數的4
  10. Pavlova viridis, isochrysis zhanjiangensis and isochrysis galbana 3011 were cryopreserved in liquid nitrogen using encapsulation - dehydration. algal cells in early stationary phase were encapsulated in 3 % ca - alginate beads with 30 nacl, 2 million cells in one bead. beads were desiccated with silica gel then directly immersed in liquid nitrogen. the cell viability after warming was evaluated by chlorophyll content. the main factors influencing the cell viability, such as water content of beads, dehydration rate, dehydration procedure, preculture and recovery methods after thawing were studied. the results are as follows : 1

    本文以綠色巴夫藻（ pavlovaviridis ） 、湛江等鞭金藻（ isochrysiszhanjiangensis ）和球等鞭金藻（ isochrysisgalbana3011 ）等三種餌料金藻為試驗材料，用包埋脫水法進行冰凍保存。選擇靜止初期的藻細胞包埋在含有30氯化鈉的3 %的褐藻酸鈣膠球中，細胞負載約200萬個細胞/膠球，經過硅膠吸濕法脫水后，探討了膠球含水量、脫水速率、脫水程序、預培養以及化凍后恢復方法對冰凍保存存活率的影響。
  11. Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells

    方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。
  12. The other 15 peptides were classified into 2 groups, each group sharing a different consensus sequence that might bind a different site of the target. one peptide of each group was chemically synthesized and added to the rdrp reaction system. we found that one peptide refrained the activity of rdrp

    自2組結合膚中各選擇1個環膚分子用化學方法合成，將合成膚加入rdrp反應體系后，其中1個環膚分子對rdrp活性表現明顯的拮抗作用，加入細胞培養中可對den感染細胞產生明顯的保護作用。
  13. With the advancement of tissue cytobiology and material engineering, by the way of tissue engineering, it is compatibile that we are going to culture the smg. the principle of the construction of tissue engineering smg is that the smg cells are isolated and cultured in vitro, and the seeded cells in the three - dimensional scaffolds

    組織工程學研究的進展，無疑給解決這一問題帶來新的希望，隨著細胞生物學和材料工程學的發展，應用組織工程學的方法，在體外培養有生物活性，且在結構和功能上與自體頜下腺相類似的組織工程化頜下腺成為可能。
  14. L lp - hsdl ma of i. 5kb was detected in the rna extracted from cult ' ured chorionic trophoblast using northem blot hybridhation method. the expression of ll6 - hsdl wa was induced by treating the chorionic cells with dexamethasone ( l0 - ', l0 ' m ) for 24h, and the induction was blocked by co - treatinent of the cells with ru486. these results suggest a gr - mediated effect of glucocorticoid on the rnrna expression and reductase activity of l l 9 - hsd1

    人h合成的肌?一加x門』 m ）對培養的絨毛膜滋養層細胞的11p hsdi還原酶活性具有誘導作用，此誘導作用可以被gr阻斷劑ru486門0川）所阻斷； northern印跡雜交方法進一步證實， dex亦可誘導絨毛膜滋養層細胞11p叫inrna的表達，此誘導作用也可以被ru486門0叫川）所阻斷
  15. The white is pure oringial liquid anne a series is french of bolanduo protect the skin research center again on leading the hairdressing item, the item is various original liquid cell live the body is to be really connected the supply by the bio - chemical graduate school development of switzerland a live composition is up to 80 % - 95 %, having to very and quickly show the effect, all products according to the standard microor gansim examinatoin of talllest pharmacy in country of france, strict quality management, assurance the qualityis prtfect

    白純原液瓶系列是法國博蘭朵護膚研究中心又一領先美容項目，項目各種原液細胞活體是由瑞士生化研究所培養直接供應，安瓶活性成分高達80 % - 95 % ，具有極快顯著效用，所有產品都依據法國最高藥學標準微生物檢驗，嚴格品質管理、保證品質完美。
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