活體細胞培養 的英文怎麼說

中文拼音 [huótǐxìbāopéiyǎng]
活體細胞培養 英文
cell culture in vivo
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 體 : 體構詞成分。
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 活體 : living body活體檢查 biopsy; 活體鑒定 [法律] identification of the living
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. We hope that our study will provide us with more comprehensive knowledge of the mechanisms of immune regulation and the roles that dc and complement play in innate and acquired immunity, as well as to lay a foundation for further exploration of the roles dc play in antigen - specific immune responses and immune tolerance from a new perspective. part i expression of complement receptors and complement - associated molecules on dendritic cells derived from distinct - origin at different stages of development two subsets of dendritic cells were generated from precursor cells isolated by means of magnetic cell separation system

    曰補體受體及其相關分子在modc上的表達不同分化階段的單核細胞衍生性dc （ monocytes一deriveddc ， modc ）的誘導:將新鮮分離的單核細胞mo ，在含有gm一csf和工l一4培養體系中誘導5一7d ，即分化為未成熟modc ;對培養至sd的未成熟modc ，用tnfa刺激zd ，即分化為成熟modc ;此時再用lps刺激24h ，即為活化的modc 。
  2. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  3. The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased

    結合以上創建的方法和免疫熒光流式細胞術，用k562細胞株可溶性分泌物（上清）對外周血單個核細胞（ pbmc ）進行培養以模擬體內微環境，然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究，結果發現用腫瘤上清培養的pbmc細胞數量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細胞數的下降主要是由細胞壞死和凋亡引起的，腫瘤上清對細胞周期沒有阻斷作用，反而略有促進作用； t細胞亞群比例增加，並促進表達th1 、 th2細胞因子。
  4. Prolonged the culture term, hepatocytes both of experimental groups and control groups became pyknotic and were detached from the wall or fibroblasts became prominent in the culture pl ate. it was important to note that none of the condition caused an increase in the number of cell in the whole experimental process. conclusion : the primary hepatocytes in medium of additional special nutritive may benefit in comparison with common medium

    結論：在培養液中添加轉鐵蛋白、牛胰島素、煙酚胺、 p琉基乙醇以及促肝細胞生長因子，可以促進原代小鼠肝細胞的貼壁生長，並且可以改善肝細胞在體外的代謝，使之維持良好的生活狀態並且延長體外生存時間。
  5. In terms of human cell cloning, he gained a great deal of experience

    在人體活細胞（克隆）培養增殖技術方面積累了豐富的經驗。
  6. During the culture of the porcine ear skin fibroblasts, the successful rate was increased by selecting culture methods, contrasting donor age and improving culture condition. the rate of living cells in the method of combining trypsin cold treatment with trypsin heat treatment was much higher than that in the method of trypsin heat treatment. the results showed that the method of combining trypsin cold treatment with trypsin heat treatment had less adverse effects on cells than the method of trypsin heat treatment

    在培養豬耳皮膚成纖維細胞時從篩選細胞分離方法、比較供體年齡和改善培養條件這三方面著手提高培養的成功率。實驗中發現胰蛋白酶冷熱處理結合法培養的細胞存活率明顯高於胰蛋白酶熱處理法，說明使用胰蛋白酶冷熱處理結合法對細胞的不利影響較少，細胞產量較高。
  7. By this method of primary culture, cells and tissues could not only subsist for 11 months and more in vitro, but also be subcultured. these works lay a foundation of the establishment of silkworm embryonic cell line

    為建立新的家蠶胚胎細胞系奠定了一定的基礎。原代培養的細胞和組織塊在體外有一定的生長現象，細胞體外最長存活達門個月以上。
  8. Splenocytes of the primed mice were restimulated in vitro for 5 days with indicated peptide loaded syngeneic splenocytes irradiated to proliferate cytotoxic t lymphocytes ( ctls )

    在體外用射線滅活的相應肽致敏的未免疫同系小鼠脾細胞與之培養5天，以刺激ctls增殖。
  9. The optimized result of cell immobilization cultures was acquired, including the support matrices pretreatment, the class and size of support matrices, the level of dissolved 62 and inoculum. the optimal ratio of hormones was got by uniform design according to its high immobilization level, high metabolism and retaining vigorous cells with long period. a medium which fit well all the conditions was obtained, and the efficiency of producing ginkgolides by cell culture improved obviously

    進行了優良種系的誘導和優選，得到了一種生長快、分散性好，並很好的符合固定化要求的細胞株系；對固定化條件進行了最優化配置- -從載體預處理、載體種類、載體量、大小和溶氧到細胞接種量系統的研究；採用均勻設計尋求一種高固定化、高代謝及長期保持細胞活力的激素配比，最終得到一種基本滿足上述要求的培養基。
  10. The expression of ha in vero cells infected with rprv - ha was detected by western - blot. the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha. the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza

    結果表明所獲得的重組病毒（ rprvha ）遺傳性狀穩定，在培養細胞中能穩定的表達與sivha具有相似生物學活性的外源蛋白，為進一步制備抗豬流感的重組偽狂犬病毒活載體疫苗奠定了基礎。
  11. Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells

    方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。
  12. The products cover the fields of the study of molecule biology, expression of protein, from cell culture to layer purification, from antigen antibody, the whole set reagent kit to the microorganism culture and the research of theactivity of the biology

    其產品覆蓋了生命領域從分子生物學研究、蛋白表達、細胞培養到層析純化，從抗原抗體、成套試劑盒到微生物培養及其生物活性研究等相關試劑幾近所有過程。
  13. The other 15 peptides were classified into 2 groups, each group sharing a different consensus sequence that might bind a different site of the target. one peptide of each group was chemically synthesized and added to the rdrp reaction system. we found that one peptide refrained the activity of rdrp

    自2組結合膚中各選擇1個環膚分子用化學方法合成，將合成膚加入rdrp反應體系后，其中1個環膚分子對rdrp活性表現明顯的拮抗作用，加入細胞培養中可對den感染細胞產生明顯的保護作用。
  14. The expression patterns of soluble proteins, peroxidase and esterase isozymes were analysed on globular embryogenesis in tissue cultures of alfalfa, it was concluded that from embryogenic callus to globular embryo genesis h peptides emerged and disappeared orderly, the patterns of peroxidase isozyme changed dramatically, esterase isozymes changed slightly while the total enzyme activity was important to keeping the potential of somatic embryogenesis

    摘要試驗在苜蓿組織培養中，對球形胚形成過程中特異蛋白質表達的模式、過氧化物酶及酯酶同工酶酶譜變化進行研究，結果表明：苜蓿組織培養中從胚性愈傷組織到球形胚發育的進程中，順序消失和出現了11種中小分子量多肽；過氧化物酶同工酶酶譜發生了顯著的變化；酯酶同工酶酶譜變化不大，但其總活力對于維持體細胞胚胎發生是必須的。
  15. In this paper, rutilt oxide film, anatase oxide film on titanium, titanium surfaces containing calcium and containing phosphorus, and apatite coating - ti were surface characterized. based on investigation on apatite precipitation, protein adsorption and osteoblast culture in vitro on the titanium surfaces, the relationship between the bioactivity and the surface characterization of titanium was studied

    在本論文中，通過金紅石二氧化鈦膜、銳鈦礦二氧化鈦膜、含鈣的鈦表面、含磷的鈦表面和ti -羥基磷灰石塗層的表徵，結合羥基磷灰石沉積、蛋白質吸附和細胞體外培養，研究了鈦的表面表徵與生物活性的關系。
  16. In order to improve titanium alloy ' s biocompatibility, the titanium dioxide coating was fabricated on ti matrix. besides, the surface of titanium alloys was directly disposed by alkali to make it possess bioactivity. biocompatibility and bioactivity of the material after surface modification were evaluated through the simulated body fluid ( sbf ) cultivation and cell cultivation experiment

    為了提高材料的生物相容性，本研究對自製的鈦基體進行了二氧化鈦塗層的制備和表面堿液生物活化處理的研究，並採用模擬體液培養實驗和細胞培養實驗評價了表面改性后材料的生物相容性和生物活性，對基體以及表面改性后材料的組織形貌、相組成和生物相容性進行了觀察、分析與評估。
  17. Effects of platelet - rich plasma on proliferation and osteogenetic activity of marrow mesenchymal stem cells in vitro

    富血小板血漿對體外培養骨髓間充質幹細胞增殖及成骨活性的作用
  18. With the advancement of tissue cytobiology and material engineering, by the way of tissue engineering, it is compatibile that we are going to culture the smg. the principle of the construction of tissue engineering smg is that the smg cells are isolated and cultured in vitro, and the seeded cells in the three - dimensional scaffolds

    組織工程學研究的進展，無疑給解決這一問題帶來新的希望，隨著細胞生物學和材料工程學的發展，應用組織工程學的方法，在體外培養有生物活性，且在結構和功能上與自體頜下腺相類似的組織工程化頜下腺成為可能。
  19. Effect of vitamin c antagonism on fluorine - induced inhibition of antioxidant enzymes activities in human lymphocyte in vit

    拮抗氟抑制體外培養淋巴細胞抗氧化酶活性的研究
  20. Compared with the cytoplasts matured in the maturation medium directly for 16 ~ 18h, the fusion rate, cleavage rate and blastocyst development of reconstructed embryos from oocytes cultured in 2mmol / l 6 - dmap for 3 - 5h prior to ivm did not differ significantly from that of reconstructed embryos from oocytes matured in the maturation medium directly for 16 ~ 18h

    水牛卵母細胞先經水牛卵母細胞孤雌激活與體細胞核移植的研究6 dmap抑製成熟培養3 sh ，再在正常成熟培養液中成熟培養16 18h 。結果發現，與直接在正常成熟培養液中成熟培養16 18h的卵母細胞相比，其融合率，分裂率和囊胚發育率均無顯著影響。
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