溶細胞因子 的英文怎麼說

中文拼音 [róngxìbāoyīnzi]
溶細胞因子 英文
cytolytic factors
  • 溶 : 動詞（溶化; 溶解） dissolve
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. Summary background data : soluble receptor proteins derived from the macrophage - monocyte lineage potentiate the inflammatory cytokine response early in ap

    背景資料：從單核巨噬細胞系來源的可溶性受體蛋白可能是急性胰腺炎早期的炎癥細胞因子。
  2. Efficiency : richly contain vitamin fresh fruit essence, deep sea squalene and vegetal whitening essence, dissolve oil and dirt, peel off aged horniness, promote cells metabolism, fade dull tone, decompose melanin and inhibit the formulation of melanin, whiten and shine skin

    功效：含豐富的維他鮮果精華、深海角鯊稀、植物美白素等，溶解油脂和污垢、退除老化角質，促進細胞新陳代謝、清除令膚色暗啞的黃黑因子，分解黑色素；同時阻斷黑色素的再次生成，美白煥膚。
  3. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  4. The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased

    結合以上創建的方法和免疫熒光流式細胞術，用k562細胞株可溶性分泌物（上清）對外周血單個核細胞（ pbmc ）進行培養以模擬體內微環境，然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究，結果發現用腫瘤上清培養的pbmc細胞數量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細胞數的下降主要是由細胞壞死和凋亡引起的，腫瘤上清對細胞周期沒有阻斷作用，反而略有促進作用； t細胞亞群比例增加，並促進表達th1 、 th2細胞因子。
  5. Construction of plasminogen activator and its expressive activity in human umbilical vein endothelial cell

    組織型纖溶酶原激活因子構建及其在人臍靜脈內皮細胞的表達活性
  6. Pcr analysis indicated that all lines had been integrated of ssmapkk. northern analysis revealed the presence of expression of ssmapkk mrna in transgenic lines. in principle, ssvp overexpression can increase proton electrochemical gradients across the vacuolar membranes, which permit the secondary active transport of na + and solute molecules

    理論上， ssop的過量表達可增加轉基因植株細胞跨液泡膜的質子電化學梯度，為次級轉運提供驅動力，從而增加可溶性物質和na十向液泡內的轉運，提高轉基因植株的抗旱和抗鹽性。
  7. 2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin

    二、在以上研究的基礎上，對cmiv抗菌肽的c端進行較大的改造，即將與腫瘤細胞過度表達的表皮生長因子受體（ egfr ）具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端，設計完整的基因，並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。
  8. Based on the theoretical analysis and experimental researches, it is presented that the wider spectra are resulted from the many fluorophores with large numbers of vibrational energy levels on the ground level in the blood cells, and the reduction of the spectral intensity is due to the reabsorption of the blood cells and the energy transfer of the collisions between the fluorophore and another one or other macromolecule. on the other hand, when the concentration of the blood cells is increased, the reabsorption of the blood cells, the secondary fluorescence due to the reabsorption and the influence of the concentration on the energy levels of fluorophores are all the factors of the red - shifted spectral peaks

    在進行理論分析和研究的基礎上，提出了因血細胞中存在多種熒光團，且這些熒光團的電子能級上又存在大量的不同的振動能級，從而導致被激發的熒光團發出較寬的熒光光譜；血細胞濃度的增大，熒光團以及其他大分子之間的距離變小，造成它們之間因碰撞的能量轉移概率加大，因而易產生熒光猝滅，結果導致熒光強度的變小；血細胞溶液中重吸收所導致的熒光猝滅和二次熒光發射，以及血細胞濃度的變化對其中熒光團能級系統的影響都是導致熒光峰值波長「紅移」的原因；進而研究了led光誘導血細胞產生熒光光譜的機理。
  9. Abstract : objective : to determine if 24 - hour blood concentrations of macrophage migration inhibitory factor ( mif ), soluble cd14, and cd163 receptors could predict complications associated with acute pancreatitis ( ap )

    摘要：目的：確定巨噬細胞移動抑制因子（ mif ） 、可溶性cd14 、 cd163受體的24小時血藥濃度能否預測急性胰腺炎（ ap ）的相關並發癥。
  10. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  11. Objectives : in our previous study, to improve the expression efficiency of human basic fibroblast growth factor ( hbfgf ) expressed in escherichia coli, we lowered the g + c content in hbfgf ' s tir region and used more preferred codons by e. coli. meanwhile we found the low insoluble product with a large quantity of dimer and higher multimer in the protein solution. it greatly decrease the bioactivity of hbfgf

    目的：我們在長期實踐中發現，大腸桿菌中表達的人堿性成纖維細胞生長因子（ humanbasicfibroblastgrowthfactor ， hbfgf ）在e . coli中表達量不高，可溶性低，蛋白溶液中大量存在二聚體，三聚體以至多聚體，使得hbfgf的生物活性大大降低。
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