病毒性酶 的英文怎麼說

中文拼音 [bìngdúxìng]
病毒性酶 英文
viral-specific enzyme
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 病毒性 : [醫學] viral病毒性肺炎 viral pneumonia; 病毒性肝炎 virus hepatitis; 病毒性感冒 virus flu
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Through expert s analysis, aloe contains rich natural protein, vitamin, chlorophyl and the neccessary microelements. it has effects of laxative, stomach care, detoxifcation, detumescence, acesodyne and diminish inflammation. so aloe is usually used to treat astriction, cold, cough, headach, car sickness, bronchia, gastric ulcer, liver disease, hypertension, diabetes, eczema, fleck, chilblain, scald, cancer, etc

    經科學分析，它含有大量天然蛋白質、維生素、葉綠素、洛性酶和人體必需的微量元素及蘆蔡大黃素等七十多種成份，具有催瀉、健胃、通經、解毒、消腫止痛、清熱抗炎等作用，對便秘、感冒、頭痛、咳嗽、暈車、支氣管、胃瘍病、小兒厭食癥、肝病、出血癥、高血壓、糖尿病、濕疹、雀斑、凍瘡、燙傷、刀傷、癌癥等數十種疾病有療效。
  3. Change of catalase activity in interaction of tobacco and cucumber mosaic virus

    煙草與黃瓜花葉病毒互作中過氧化氫酶活性的變化
  4. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks

    生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。
  7. The homology of the other two motifs ( v and vi ), which are also quite conserved in other helicases, were lower than 30 %. the hasn pv helicase protein had considerable amino acid sequence similarity with other baulovirus helicases ( 58 % ), and the highest ( 66 % ) with semnpv and the lowest with xcgv. ( 43 % ). hasnpv helicase was the first helicase reported in single - nucleocapsid nucleopolyhedrovirus there are 5 homologue regions ( hrs ) in hasnpv genome which may play important roles in the viral replication

    同源性比較發現hasnpv解螺旋酶的氨基酸序列與甜菜夜蛾核多角體病毒（ spodopteraexiguemnpv ， semnpv ）的解螺旋酶具有最高的同源性（ 66 ） ，與xestiac - nigrum顆粒體病毒（ xcgv ）解螺旋酶同源性最低（ 43 ） ， hasnpv解螺旋酶基因是第一個報道的單粒包埋核多角體病毒的解螺旋酶基因。
  8. Correlation between signal cutoff ratios of anti - hcv enzyme immunoassay and their true positivity in blood donors

    獻血員丙型肝炎病毒抗體酶免疫法檢測試劑的測量值與其真陽性的相關性
  9. Studies on virus resistance of transgenic tobacco with tmv replicase gene

    轉煙草花葉病毒復制酶基因煙草的病毒抗性研究
  10. Only antibody of e of second liver virus and core antibody two positive, transaminase is normal also, this does not have the requirement of second liver

    僅乙肝病毒e抗體和核心抗體兩項陽性，轉氨酶也正常，這不具備乙肝的條件。
  11. The main bioactivities include antibacterial, antimicrobial, antitumor, antiviral activity, enzyme and enzyme inhibition, and antitumor activity is more important

    其生物活性包括：抗菌、抗腫瘤、抗微生物、抗病毒、酶及酶的抑制活性等，其中以抗腫瘤活性最為重要。
  12. In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not

    合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。
  13. The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production

    應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。
  14. Christian d, stephan g, wolfgang p, et al. identification of a novel coronavirus in patients with severe acute respiratory syndrome [ j ]. engl j med, http : / / content. nejm. org / cgi / reprint / nejmoa 030747v2. pdf, 2003 - 0410

    吳新偉，程鋼，狄飈.熒光聚合酶鏈反應檢測嚴重急性呼吸綜合征冠狀病毒的方法建立及臨床初步應用[ j ] .中華檢驗醫學雜志， 2003 ， 26 : 300 - 302
  15. Studies suggest that it probably interacts directly with the viral polymerase, and we want to confirm that by mutagenesis and by enzymatic activity assays, " said tao

    有研究表明，核蛋白可能會和病毒的聚合酶直接發生相互作用，我們將通過人工誘變技術及酶活性測定等技術來核實這一說法。 」
  16. Protocol of reverse transcription polymerase chain reaction rt - pcr for infectious haematopoietic necrosis virus

    傳染性造血器官壞死病毒逆轉錄聚合酶鏈式反應操作規程
  17. Analysis on myocardial enzymes in patients with vival encephalitis : a report of 60 cases

    病毒性腦炎時心肌酶譜的分析附60例報告
  18. Protocol of detection antibodies against avian viral arthritis virus - enzyme - linked immunosobert assay

    雞病毒性關節炎抗體檢測方法.酶聯免疫吸附試驗
  19. Changes of tumor necrosis factor and neuron specific enolase in cerebrospinal fluid of children with viral encephalitis

    兒童病毒性腦炎腦脊液腫瘤壞死因子和神經元特異性烯醇化酶的變化
  20. Methods : extracting the total rna of human pbmc, the objective include 60 healthy blood donator, 30 patient with viral encephalitis and multiple sclerosis and parkinsonian syndrome, 30 patient with schizophrenia and affective disorder, this indviuals were inpatients or outpatients of the first hospital of chongqing university of medical science from december, 2000 to june, 2001. using nested rt - pcr techique to detect borna disease virus " middle fragment in orf i, and using southern blot hybridization to analyze the pcr product

    重慶醫科大學碩士學位論文方法：提取從2000年12月至2001年6月在重慶醫科大學第一附屬醫院神經科和精神科住院及門診的60例健康獻血者、 30例包括原因未明的病毒性腦炎、多發性硬化、帕金森綜合征，以及30例包括精神分裂癥和情感障礙患者pbmc中的總kn 』 a ，採用套式逆轉錄聚合酶鏈反應estedrticr ）技術進行了bdvorf基因中部片段的檢測，並對pcr產物進行電泳分析及southernblot雜交分析。
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