病毒株特異性 的英文怎麼說

中文拼音 [bìngdúzhūtèyìxìng]
病毒株特異性 英文
viral strain-specificity
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 株 : Ⅰ名詞1. （露在地面上樹木的根和莖） root and stem of a tree above the ground 2. （植株） individual plant; plant Ⅱ量詞（棵）
  • 特 : Ⅰ形容詞（特殊; 超出一般） particular; special; exceptional; unusual Ⅱ副詞1 （特別） especially; v...
  • 異 : 形容詞1 （有分別; 不相同） different 2 （奇異; 特別） strange; unusual; extraordinary 3 （另外的;...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 病毒株 : virus strain
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  • 特異性 : distinction
  • 特異 : 1 （特別優異） exceptionally good; excellent; superfine2 （特殊） peculiar; distinctive特異功能 s...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain

    本實驗採用spf雞胚及雞胚原代成纖維細胞，從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞，並且這種凝集可以被特異性抗血清所抑制。
  3. The two isolates were positive reaction hi polymerase chain reactions with two pair of primers specific for alv - j and gave antigenically strong reaction hi the indirect fluorescence antibody assay ( ifa ) with alv - j specific monoclonal antibody je9. negatively - stained electron microscopic and immune - electron microscopic observation demonstrated that viral particles of the inner mongolia and shandong isolate of alv - j, respectively designated imc10200 and sdc2000 strain of alv - j, showed characteristic morphology of alv

    利用pcr和間接免疫熒光反應進行鑒定，兩株j亞群禽白血病病毒可以被兩對alv - j特異性引物擴增（特異條帶約2 . 2kb和545bp ） ，且在特異性單克隆抗體je9的間接免疫熒光檢測中呈現強陽性熒光反應。
  4. " there may have been something about that particular season in terms of either natural variation in susceptibility patterns going on but also then potentially this low - level community transmission of variants with reduced susceptibility as well, ' ' he said

    他說： 「也許是在特殊的季節，病毒的敏感譜發生了自然變異，或者敏感性降低的變異病毒株在社區內低水平地傳播。 」
  5. The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous, but lowly homologous with other reference strains. the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains, and is identical with the facts in the field cases. all the guangxi isolates are classified into genotype vii of apmv - 1, the same genotype dominated in china and other areas in recent years

    結果發現，廣西分離株之間在信號肚的核旮酸和氨基酸同源性很高，而與其它參考株差異較大；廣西分離株在裂解位點的氨基酸組成和排列均符合強毒株的特徵，並與毒株在臨床上的致病情況相符；根據apmvlf基因第47位第420位核苦酸序列所繪制的系譜樹吵ylogenetictree ）來看，廠西雞和鵝分離株都歸屬于基因型vll 。
  6. By morphologic examination, structure characteristics ^ biological property n serological tests and pathology characteristic study, this virus was identified as the medium virulence chicken. new castle disease virus ( ndv ), whose pathology characteristics were appeared different from those of the standard virulence ndv. la sola vaccine could not give good protection against the infection of this virus isolate

    用spf雞胚在吉林省患病雞群中分離到一株病毒，經病毒形態學、結構特徵、生物學、血清學及致病性等試驗研究，確定該病毒為雞新城疫強毒，對lasota免疫雞的致病性及其致死率與雞新城疫標準強毒e _ 9f _ （ 48 ）對比差異顯著， lasota疫苗對該株病毒免疫保護效果不理想。
  7. The antigenic and genetic variability of porcine reproductive and respirators syndrome virus ( prrsv ) isolates in china were studied by immunofluoresent monolayer assays ( 1fma ) and restriction fragment length polymorphism ( rflp ) of reverse transcription ( rt ) and polymerases chain reaction ( pcr ) amplified - prrsvorfs fragments among 8 chinese isolates

    本研究通過對豬繁殖與呼吸綜合征病毒（ prrsv ）國內分離毒株的gp3 、 gp5和n蛋白的抗原性比較及其orf5和orf7遺傳變異性分析，系統研究了國內分離毒株的抗原特性和遺傳學差異。
  8. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  9. This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv

    為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。
  10. The gene cloning and sequence analysis of senv - d and senv - h isolated from china according to the published nucleotide sequences of sen viruses, specific primers were designed and synthesized. from the serum of two chinese patients with non - a - e hepatitis, one senv - d isolate named senv - d - bj1 spanning the complete coding region was amplified by semi - nested pcr, another isolate named senv - d - bj2 spanning the partial coding region ( including orf1 and orf2 ) was amplified too. from one blood donor serum, two senv - h isolates named senv - h12 - 1 and senv - h 12 - 2 spanning the complete coding region were amplified by nested pcr respectively

    Sen病毒d和h亞型中國分離株的克隆及序列分析我們在前期工作的基礎上，結合已發表的文章及基因序列，針對senv - d和senv - h基因組設計特異性引物，利用套式pcr技術從兩例non - a - e肝炎患者血清中分段克隆得到了一個senv - d亞型分離株（ senv - d - bj1 ）的全部編碼區基因序列，還得到了一個senv - d亞型分離株（ senv - d - bj2 ）的大部分編碼區基因序列（包括orf1和orf2 ） ；從一例健康人血清中分段克隆得到了兩個senv - h亞型分離株（ senv - h12 - 1和senv - h12 - 2 ）的全部編碼區基因序列。
  11. When inoculated into 11 - day - old meat - type chick embryos, alv - j imc10200 and sdc2000 strain induced typical ml occurring after 42 weeks post - hatching

    將分離毒株回歸感染肉雞， 21周齡時特異性引物pcr抽查檢測，抽查感染雞全部為陽性； 42周齡后出現典型ml病例。
  12. The sequences of the structural protein genes and deduced a mino acid sequence of isolate lx4 were compared. by computer software, complete main structural genes sequence of ibv domestic strain and molecular characteristic genetic - variant analyses, and probably t cell and b cell epitopes of the main structural protein of infectious bronchitis virus were analyzed premently

    通過計算機分析軟體，對我國ibv地方流行毒株lx4的主要結構基因全序列、分子特徵及遺傳變異進行分析比較，並初步分析預測傳染性支氣管炎病毒主要結構蛋白上可能存在的t細胞和b細胞表位。
  13. The amplified e2 fragments of two hcv strains were all 1280bp in length by 2 % agrose gel electrophoresis. expected size of 1280bp of 2 fragments and their specificity were confirmed by restriction endonuclease digestion and then they were cloned respectively into pmd - 18t vector

    以此病毒rna為模板，利用rt - pcr技術，擴增出了豬瘟野毒株hcv - jn 、 hcv - yc完整的e2基因，用pmd - 18t載體克隆，經電泳檢測、酶切分析及pcr鑒定初步證實了所擴增片段的特異性。
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