白粒的 的英文怎麼說
中文拼音 [báilìde]
白粒的
英文
white grained-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。In vitro study of peripheral blood polymorphonuclear leukocyte degranulation induced by the filtrate of ultrasonic pulverization from actinobacillus actinomycetemcomitans
伴放線放線桿菌超聲粉碎濾液誘導外周血中多形核白細胞脫粒的體外研究The light reflected and refracted several times by a droplet and then finally returned to the observer is therefore white.
光由粒子反射和折射數次,最後到達觀察者,所以是白色的。The knowledge about higher plant mitochondrial ribosomes is scanty.
關于高等植物線粒體核蛋白體的知識也少的可憐。Introduction telomerase is a ribonucleoprotein reverse transcriptase that synthesizes telo - meric dna sequences using its rna as template. it can remedy the loss of te - lomere after cellular mitosis, maintain telomere length and stabilize chromosome
前言端粒酶( telomerase )是一種核糖核蛋白逆轉錄酶,能以自身的rna為模板,從頭合成染色體末端的端粒dna ,彌補細胞分裂時端粒dna的丟失,維持端粒的長度並穩定染色體。Pepper piper nigrum l., whole or ground. specification. part 2 : white pepper
整顆粒的和或磨成粉胡椒.規范.第2部分:白胡椒Molecular systematics of partial species belonging to 5 subfamilies of miridae are investigated in this study by using esterase isozyme ( est ) and cytochrome b ( cyt b ) gene in mitochondrial dna as molecular marks
運用酯酶同工酶和線粒體dna中編碼細胞色素b蛋白質的基因cytb作為分子標記,對盲蝽科miridae的5個亞科的部分種類進行了分子系統學研究。Bonification and centrifu - gation were recommended for the lysis of collected cells and the supernatant and precipitation were collected respectively. as to the plenty of include bodies in the precipitation, denationalization, detergence, purification and dissolution, last regeneration were recommended to acquire great deal of expressed gst fusion proteins
Coltblzi生產融合蛋白,重組后的dna序列包括一個pgex質粒,依照pgex質粒的融合蛋白的表達是在tao啟動子的控制之下,而枯啟動子可由乳糖的類似物i剛來誘導它表達。Then the plasmid was extracted from them and determined by dna calculator. the protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1 % glutaral, pay it to electric stimulation in muscle of injection site and extract omni - rna from injection site of rabbit muscle, expression of grf were detected by reverse transcription and pcr ; ratio of expression of grf were detected by elisa. extract dna form injection site of mouse muscle to research time of expression
本實驗是將含grf重組質粒的jm109菌株大量培養,用堿裂變法提取質粒,用dnacaculator定量;制備含grf的原生質體,經1的戊二醛處理后注射於家兔肌肉,在注射部位給予電刺激,提取總rna ,用rt - pcr檢測grf在肌肉中的表達;用elisa法定性檢測grf在肌肉中蛋白質水平的表達;將該原生質體注射于小鼠肌肉中,定期提取dna ,初步探討原生質介導的外源性grf在小鼠肌肉中的表達時間。According to whether remnant grains exist, post - penecontemporaneous dolostone can be divided into remnant grain dolostone and crystalline dolostone
準同生后白雲巖根據殘餘顆粒的有無可分為殘餘顆粒白雲巖和晶粒白雲巖兩大類。In our study we have cloned the osd gene from s. typhimurium by pcr, characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd
再將hpylori尿素酶b亞單位基因與尿素酶b和熱休克蛋白a融合基因分別克隆入ptrc99a一asd質粒的多克隆位點之內。Goat anti - human ige antibody were used as second antibody to make sure that the positive clones were ige related. through three cycles of screening, the inserted cdna fragments of the positive clones were amplified by pcr and sequenced. the results showed that the inserted cdna fragment from one clone was 1200 bp in length, with a orf of 507 bp which encoded 169 amino acids
Sj43b pgex 6p 1重組質粒的誘導表達、表達產物的鄉寸和免疫學性質鑒定分析為獲得可溶性的rsj43b月6gsta蟲合蛋白,對不同iptg誘導劑濃度、誘導表達溫度和誘導表達時間等因素對融合蛋白可溶性表達的影響進行了觀察。Robinson p j, dunnill p, lilly d m. preparation of fine magnetic particles and application for enzyme immobilization. ibid, 1973 ( 15 ) : 603
余藝華,薛博,孫彥,等.殼聚糖親和磁性毫微粒的制備及其對蛋白質的吸附性能研究.高分子學報, 2000 ( 3 ) : 340 344The extent of the influence that concentration, incubation time and free folate exerted on the uptake of folate - bsanp by ovarian cancer cells ( skov3 ) was determined using fluorescence spectrophotometer
以螢光定量法確定濃度、時間、游離葉酸對腫瘤細胞攝取葉酸偶聯白蛋白納米粒的影響程度。Uptake of folate - bsanp by cancer cells was gadually increased with the extension of incubation time or the increase of folate - bsanp concentration, and the uptake could be competitively inhibited by excess free folate
葉酸偶聯白蛋白納米粒的腫瘤細胞攝取量隨納米粒濃度增大、培養時間延長而增加,腫瘤細胞對葉酸偶聯白蛋白納米粒的攝取可被過量的外源性游離葉酸所抑制。According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此,本試驗首先擴增出整合在酵母基因組里的人血清白蛋白( hsa )基因作為目的基因,並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體,以構建整合型載體,再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。It can be make into leather soften agent, and such soften agent can make leather soft, clean, pore exquisite and comfortable handle
可製成皮革軟化劑,經木瓜酶軟化的皮革柔軟、潔白、粒面細致,手感好。[ leather processing industry ] it can be make into leather soften agent, and such soften agent can make leather soft, clean, pore exquisite and comfortable handle
可製成皮革軟化劑,經木瓜酶軟化的皮革柔軟、潔白、粒面細致,手感好。In those layers of leaves, dotted with white flowers here and there, some are gracefully in bloom, some shyly in bud, like scattered pearls, like stars in the clear sky, like beautiful girls just after a bath
層層的葉子中間,零星地點綴著些白花,有裊娜地開著的,有羞澀地打著朵兒的;正如一粒粒的明珠,又如碧天里的星星,又如剛出浴的美人。The bulkheads, all painted in clear white, and beaded round with gilt, bore a pattern of dirty hands
漆成純白嵌著金色珠粒的艙壁上留著泥手印。分享友人