真菌基因 的英文怎麼說

中文拼音 [zhēnjūnjīyīn]
真菌基因 英文
fungal gene
  • 真 : Ⅰ形容詞（真實） true; genuine; real Ⅱ副詞1 （的確; 實在） really; truly; indeed 2 （清楚確實） cl...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. Isolate all grew well in the culture medium with initial ph 4 - 10, the optimal growth temperature range was from 28 to 30. 5 degree c. it grew well on the medium for fungi growth, such as pda medium and czpek medium etc, and also grew well on the cause ' s i medium and the non - nitrogen medium, but little growth on the luria bertani medium ( lb ) and beef extract peptone medium. it did not need special nutrition factors for growth, but source of the carbon was the key factor to growth, all of its nutrition needs were different from that of common bacteria

    該菌在初始ph4 - 10的培養基中都能夠生長，生長最適溫度范圍為28 - 30 . 5 ，在pda 、查氏等真菌培養基中生長旺盛，在高氏1號和無氮源培養基中同樣生長良好，而在lb與牛肉膏蛋白腖等細菌培養基中生長很差，碳源是其生長的關鍵因子，這有別於一般細菌的營養需求。
  3. His research interests lies in : 1 ) insect fungal pathogenesis and strain improvement by genetic modification ; 2 ) fungal conidiation, conidial germination and dimorphism

    研究領域： 1 ）昆蟲病原真菌致病機理和基因工程研究； 2 ）真菌孢子發育和形態轉換的分子機理研究。
  4. The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide

    核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。
  5. Like eukaryotes, but unlike bacteria, they have introns in their transfer rna, but like bacteria they have polycistronic operons ( gene regulators )

    像真核生物而不像細菌，轉運rna中含有內含子，還有細菌不具備的多順反子的操縱子（基因的調控子） 。
  6. Plant defensin disrupts the cell membrane of phytopathogen differently from cema that contacts directly with the cell membrane. plant defensins are thought to interact with specific receptor harboring in the cell membrane

    由於對真菌，特別是絲狀真菌有很強的抑制作用，而對植物細胞沒有毒害作用，防禦素有望在植物抗病基因工程中得到廣泛應用。
  7. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  8. The resulting derivative strains produced antifungal activity in a manner different from the parental strain, indicating the original promoter was replaced with the engineered promoters and the epitopes

    衍生菌株合成抗真菌抗生素的產量與親本菌株不同，表明fr - 008基因的原始啟動子已被改造后的啟動子和抗原決定簇所代替。
  9. Expression of this truncated gene in plant was expected to give information about expression in plant of high g + c content genes but no antifungal activity was expected in this stage

    由於載體中2 . 7kb的pks基因編碼了兩種酶活性的結構域而不是完整的型pks模塊，因此我們目前並不期望這個截短的pks基因在植物能產生抗真菌活性。
  10. The three fungi also differed in base exchange capacity, with paxillus involutus having the highest base exchange capacity and suillus bovinus the lowest

    供試的三種菌根真菌的菌絲都具較大的鹽基代換量值，因而具有較強吸附重金屬潛力。
  11. The results showed that the base exchange capacity of the three ectomycorrhizal fungi was much higher than cation exchange capacity of plant roots, indicating that the fungi may have great potential to adsorb heavy metals

    菌根真菌耐重金屬的能力與真菌吸附重金屬的能力有關，因而需要了解菌根真菌對重金屬吸附和固持的特性。真菌的吸附能力在一定程度上受真菌鹽基代換量影響，因此測定二種真菌的鹽基代換量。
  12. Armed with modified antibacterial peptide ( spcema and mcema ) and plant defensin afp, transgenic plants with broad - spectrum and strong disease resistance may be obtained

    以上三個單價和一個雙價抗菌肽表達載體在植物中的遺傳轉化，有望獲得對病源真菌有廣譜抗性的轉基因植株。
  13. Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses

    沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
  14. Mechanism of fungal pathogenesis in insect and strain improvement by gene engineering

    昆蟲病原真菌致病寄主的機制和基因工程改良
  15. Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa

    將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。
  16. The modified sequence was cloned into binary vector pbi121. the constructed expression vector was named pbi121 - bar, then transferred into agrobacterium tumefaciens lba4404 by triparental crossing. thus the project bacterium has been successfully constructed

    改造后的目的基因克隆于真核表達載體pbi121 ，構建表達載體pbi121 - bar ，通過三親雜交法將真核表達載體pbi121 - bar轉入農桿菌lba4404 ，成功構建出工程菌。
  17. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  18. Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag

    目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。
  19. This paper summarizes the commonly used methods for the research of gene function of filamentous fungi, such as transposon tagging, gene knockout, rna interference, over - expression and yeast hybrid system, and provides a discussion on the advantages and disadvantages of those methods

    本文總結了目前絲狀真菌基因功能研究中常用的方法，如轉座子標簽法、基因敲除技術、 rna干擾、超表達及酵母雜交系統等，並對各方法在絲狀真菌研究中的優缺點進行了闡述。
  20. The new molecular biology methods applied for genotyping medical fungus

    新的分子生物學方法用於真菌基因型鑒定
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