硫酸胍 的英文怎麼說
中文拼音 [liúsuānguā]
硫酸胍
英文
guanidine sulfate-
Our efforts were taken to lay the foundation of further studies on cloning and function of new genes in fish. the construction and evalution of the a, gtlo cdna library of grass carp leukocytes are described. total rna was extracted from head - kidney leukocytes using single - step method of rna isolation by acid guanidinium thiocyanate - phenol - chloroform extraction
本文第一部分取病毒感染36小時后的草魚頭腎並分離白細胞，用改進的異硫氰酸胍一步法從其中提取總rna ，磁珠法分離純化mrna ，電泳檢測其質量。In this paper, the structures and the synthesis methods of glycosyl isothiocyanates are summarized, including the application as intermediates in the preparation of glycosylthioureas, glycosylheterocycles, glycosylguanidins and glycosylconjugates and so on
本文對糖基異硫氰酸酯的結構、制備方法，以及作為中間體在糖基硫脲、糖基雜環、胍基糖苷、糖基綴合物等重要化合物的合成中的應用作了較詳盡闡述。In the experiment, the first instar housefly larvae " metallothionein mrna expressing reach maximur. after induced by cd ( 2mm ) for 48 hours, then total rna was extracted with guanidinium isothiocyanate, and mrna was isolated by the polyattract mrna isolation systems surplied by promega company
試驗中採用2mmcd ~ 2對家蠅初孵幼蟲誘導48小時，使其體內金屬硫蛋白mrna表達達到高峰，在液氮中將其研磨成粉末后採用異硫氰酸胍法提取總rna ，再用promega公司提供的小量分離mrna試劑盒純化mrna 。The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。In this research, total rna was extracted from fetal liver by the modified single - step method of acid guanidinium thiocyanate - phenol - chloroform ( agpc ). based on reported sequence of hsa cdna, specific primers were designed to amplify its encoding region by rt - pcr. at the same time, kozak sequence was added to the translation site, to strengthen initiation of translation
本研究通過改進的異硫氰酸胍-酸性酚-氯仿一步法從人工流產胎兒肝臟中提取總rna ，然後根據報道的序列設計特異性引物，同時在上游引物中引入了kozak序列，經rt - pcr克隆到hsacdna編碼區序列，長度為1758bp 。分享友人