稀釋基因 的英文怎麼說

中文拼音 [xīshìjīyīn]
稀釋基因 英文
dilution gene
  • 稀 : Ⅰ形容詞1 （事物出現得少） rare; scarce; uncommon 2 （事物之間距離遠; 空隙大） sparse; scattered 3...
  • 釋 : Ⅰ動詞1 （解釋） explain; elucidate 2 （消除） clear up; dispel 3 （放開; 放下） let go; be reliev...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 稀釋 : [化學] dilution; thinning; attenuation; deliquate; dilute稀釋處理化 dilution; 稀釋劑 diluent; att...
  1. An allele can not be “ watered down ” by outcrossing

    一個基因等位體不會由於與異體通婚而被稀釋。
  2. Used the genomic dna extracted by low melting - point agarose embedding method as pcr template, the full length of structural genes of bacillus subtilis bio operon were gained by long pcr method

    將該方法提取的基因組dna稀釋100倍作為模板，採用長距離pcr方法，獲得了枯草桿菌生物素操縱子基因全長。
  3. The research consist of four parts. the first part is multiplication, purification and electron microscope examination of the avian encephalomyelitis virus. a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos. respectively. after incubation for 10 days, the urinay vesicle liquid was collected. making a comparison the size of the chickens embryos between the test group and the control group, the results showed that the size of the control group is bigger than that of the test group. purified virions were examined under the electron microscope, the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos. the second part was seguence analysis of the genome of the aev - nh937. nine pairs of primers were designed according to published calnek vaccine strain of aev

    本研究共分四個部分：第一部分為aev的增殖，純化和電鏡觀察，用1 : 5倍稀釋的aev - nh937株和陰性對照pbs分別經卵黃囊接種於6dspf雞胚，繼續孵化10d后，收集尿囊液。比較接種組和健康對照組雞胚的大小，結果顯示，健康對照組雞胚明顯大於接種組。分離、提純aev ，把純化的病毒在電鏡下觀察，證明確有大量aev病毒粒子存在，說明aev在雞胚中成功擴增；第二部分是aev - nh937基因組的序列測定工作。
  4. Gfral was high expressed in e. coli after iptg induction. by ni2 + chelation affinity chromatography, the purified recombinant gfral protein were obtained. based on the evolutionary trace method, multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family

    2 . gdnf的表達及其對pc12基因工程細胞的影響用本教研室已構建好的表達質粒pet一gdnf轉化大腸桿菌bl21 （ de3 ） ，經lmmipto誘導gdnf表達，並在niz氣nta柱上進行純化，稀釋復性后，純度達90 %以上。
  5. 2. cloning of structural genes of bacillus subtilis bio operon diluted the genomic dna of bacillus subtilis as the template, long pcr product ( 10. 3kb ) and three salvage pcr products were separately gained by optimization of reaction conditions of pcr

    枯草桿菌生物素操縱子基因的克隆將枯草桿菌基因組dna稀釋后，通過pcr反應條件的優化，分別擴增得到了生物素操縱子基因的長距離pcr產物（ 10 . 3kb ）和3個分段pcr產物。
  6. By increasing the h2 dilution ratio, it is found that atomic hydrogen can selectively etch amorphous phase and stabilize crystalline phase. from the study on the distance from substrate to catalyzer, choosing a proper distance can ensure the gas fully decomposed, while a relatively low substrate temperature can cause the nanocrystalline particles to lose mobility and keep their sizes. the pre - carbonization process can enhance the nucleation density and make the growth of high quality nanocrystalline p - sic films much easier

    實驗結果表明：隨著工作氣壓的減小，薄膜的晶粒尺寸有所減小；通過提高氫氣稀釋度，利用原子氫在成膜過程中起的刻蝕作用，可以穩定結晶相併去除雜相；選擇適當的熱絲距離能保證反應氣體充分分解，又使襯底具有較高的過冷度，是形成納米薄膜的重要條件；採用分步碳化法可以提高形核密度，有利於獲得高質量的納米- sic薄膜；襯底施加負偏壓可以明顯提高襯底表面的基團的活性，因負偏壓產生的離子轟擊還能造成高的表面缺陷密度，形成更多的形核位置。
  7. Aim : to analyze the mechanism, thermadynamic theoretical basis, dynamic mechanism and influencing factors of thermally induced phase separation ( tips ) in order to completely grasp the factors affecting the size, distribution and form of pores, so that the adjusted range of pore can be widened and the preparation of porous membrane can be repeated and controlled. methods : considering from the structural characteristics of tissue engineered materials, the methods of preparing porous membrane using tips technique, the hermadynamic theoretical basis, dynamic mechanism and influencing factors were analyzed, the problems and investigative directions in the future were also analyzed. tips technique is a process of phase separation of polymer homogenous solution under quenching, and it is suitable for diameter and structural form of the micropore materials prepared using tips are closely correlated with the kind and dispensing proportion of polymer attrnuant, polymer concentration and polymer molecular mass, etc. conducted, including determination of polymer - solvent system phase diagram, study of form and appearance of porous membrane of different thickness, study of form and appearance of porous membrane prepared with systems of different x, which is the parameter of polymer - solvent interaction

    目的:分析熱致相分離成膜過程的機理、熱力學理論基礎、動力學機制以及影響因素，以便充分掌握影響孔度大小、分佈、形態的因素，使孔度調控范圍得以拓寬，使多孔膜的制備能重復可控.方法:從組織工程材料結構特點出發，分析熱致相分離聚合物多孔膜的制備方法及該法成膜的熱力學理論基礎、動力學機制以及影響因素.並分析實驗中存在的問題及今後的研究方向.結果:以熱致相分離法可制備聚合物多孔膜.熱致相分離法制備多孔膜是高聚物均相溶液在淬冷條件下發生相分離的過程，它適用於上臨界共溶溫度型聚合物一稀釋劑二元體系.熱致相分離法成膜的過程，可以認為是旋節線機理佔主導地位.熱致相分離法制備的微孔材料，其孔隙率、孔徑大小、結構形態與聚合物稀釋劑的種類、組成配比、聚合物濃度、聚合物分子量等因素密切相關.結論:可採用熱致相分離技術制備多孔膜，通過改變不同的成膜條件可獲得一系列不同孔徑尺寸和孔徑分佈的多孔膜材料.對熱致相分離成膜過程中聚合物-溶劑體系的相圖測定，不同厚度的多孔膜形貌研究，不同x （聚合物-溶劑相互作用參數）體系所制備的多孔膜形貌等需深人研究
  8. Dodecyl glycidyl ether is a good reactive diluent with low toxicity and no irritation. the studies were concentrated on the synthesis and the factors effecting the quality of the diluent

    摘要對稀釋劑十二烷基縮水甘油醚的合成及影響產品質量的因素進行了研究，並以十二、十四混醇為原料進行了對比試驗。
  9. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  10. Essential oils in the pure state are too highly concentrated to be used directly on the skin, therefore, dilute in a carrier oils vegetable oils so that they can be massaged or rubbed on to the skin in the correct dosage

    基礎油基礎油就是將精油加以稀釋的植物油，因為純精油的刺激性十分強烈，直接擦在皮膚上，會造成傷害，所以精油在皮膚上使用前，一定要先稀釋。
  11. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
分享友人
分享到 Line

分享到臉書