穿梭載體 的英文怎麼說

中文拼音 [chuānsuōzǎitǐ]
穿梭載體 英文
shuttle vectors
  • 穿 : Ⅰ動詞1 （破; 透） pierce through; penetrate 2 （通過孔、隙、空地等） pass through; cross; go thro...
  • 梭 : 名詞（梭子） shuttle
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 穿梭 : （像織布梭子一樣來回） shuttle back and forth
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate

    將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。
  2. The expression plasmid called psugv - badfe was constructed by inserting ba - dfe gene into e. coli - b. subtilis shuttle vector psugv4 and the

    將ba0fe基因克隆到大腸桿菌一枯草桿菌穿梭載體psugv4中，得到重組質粒psugv badfe ，然後轉化到枯草桿菌wb600中進行了分泌表達。
  3. With the aim of increasing the expression and stability of mtxl, the mtxl gene originated from b. sphaericus ssii1 was cloned to a shuttle vector pbu4. two recombinant plasmids pmt9 and pmt4 were obtained, with the inserted fragments in the opposite orientations

    為了提高mtx1殺蚊毒素蛋白的表達量和穩定性，本文將來源於球形芽孢桿菌b . sss - 1的mtx1毒素基因克隆至穿梭載體pbu4上，得到mtx1插入方向相反的重組質粒pmt9和pmt4 。
  4. The sacvgb gene was then cloned into e. coli - - b. sublilis shuttle vector psugv4 and a recombinant plasmid named psu - sacvgb was constructed. after transformation with b. sublilis wb600, the transformants successfully produced vhb intracellularly. the influence of vhb on b. subtilis is under investigation

    將sacvgb基因克隆到大腸桿菌-枯草桿菌穿梭載體psugv4中，獲得重組表達質粒psu - sacvgb ，然後轉化枯草桿菌wb600 ，轉化子經2蔗糖誘導，該基因在枯草桿菌中表達。
  5. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  6. Genoraic dna of b. megaterium was partially digested by restriction enzyme sau3a and was used to establish the genomic library in plasmid pbluscripts. selection for endoglucanase positive clones from transformed e. coli was carried out on the cmc medium. seventy - four clones that showed hydrolysis ability on the cmc plate were obtained

    用ecori和sall對含有幾丁質酶基因的重組質粒tvchi （含幾丁質酶編碼基因的pmd18一t載體）和穿梭質粒phy300plk進行雙酶切並連接，轉化e . colidhs 。
  7. Construction of corynebacterium glutamicum e. coli shuttle promoter - probe vector

    大腸桿菌穿梭型啟動子探測載體構建
  8. We constructed 3 kinds of shuttle plasmid encoding hcv structural proteins by means of molecular cloning technique ; based on it, 3 kinds of adenovirus vectors of hcv structural proteins were packed, screened and identified. we also selected and synthesized 5 kinds of ctl epitope peptides of hcv structural gene which induced 5 kinds of corresponding hcv specific ctl, which were evaluated by experiments in vivo and in vitro

    本研究利用分子克隆技術，構建了三種hcv結構蛋白腺病毒載體穿梭質粒，並以此為基礎包裝、篩選和鑒定了三種hcv結構蛋白腺病毒表達載體，選擇併合成了hcv結構基因區5條ctl表位多肽，並以此誘導了5種hcv特異性ctl ，並對其進行了體內外的實驗測定。
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