純化的 的英文怎麼說

中文拼音 [chúnhuàde]
純化的 英文
clean
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 的 : 4次方是 The fourth power of 2 is direction
  • 純化 : purification; purifying; depuration; edulcoration; purify
  1. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum

    使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。
  2. The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27. it was released from the cbd fusion tag efficiently

    利用融合蛋白中目的蛋白lt 27與cbdtag接頭處的腸激酶特異性識別位點，用腸激酶處理粗純化的融合蛋白，可將卜
  3. By co - transfection of the mutants with full - length raplgap, we found the mutant at 82 position could still bind to full length raplgap, while the mutants a

    本文採用a衛中標記的gtp與純化的rapla共孵育，使rapla全部與放射性同位素標記的gtp結合。
  4. The substance with antibacteria action obtained from forest frog is made up of alanine, aminoacetic acid, leucine, isoleucine, proline, aminoglutaric acid, threonine, serine, lysine. the substance with antibacteria action is a kind of poly peptide with a micromolecul

    純化的林蛙皮膚抗菌活性物質經尿素? sds ? page電泳分析，表現為一條帶，分子量約為6 . 28kda 。
  5. Several method for identification, separation and purification of hemocytes in lepidoptera

    鱗翅目昆蟲血細胞鑒定與分離純化的幾種方法
  6. The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e. coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method. several conditions, which include diffend ph buffer and concelltheion of imidazole, were selected to purify recombinan nucleorotein

    根據載體pproexhtb含有（ his ） 6特點，將融合蛋白進行純化，在純化過程中經各項條件的探索，確定為在裂解液中含有1mmpmsf的條件下，分別經過2倍體積的buffera和bufferb洗脫后，再收集ph5 . 9 ，含有80mmol / l咪唑的1倍體積bufferc洗脫液，可得到純化的融合蛋白。
  7. In the part, the author ' s intention is mensurating the molecular weight of the polypeptides by sodium dodecyl sulfate polyacrylamide gel electro - phoresis. the result shows that one of the polypeptides which we have mensurated is about 17, 000da. the conclusion is that the components which we have separated and purified are small molecule polypeptides. 5

    紅褐林蟻多肽蛋白質的分子量測定本部分旨在通過sds -聚丙烯酰胺凝膠電泳測出所得多肽蛋白質的相對分子量，測得其中一組分的相對分子量為17000da左右，結論為所分離、純化的物質是小分子的多肽。
  8. Solubilization is required of any protein to be purified.

    增溶溶解對于任何要純化的蛋白質都是必需的。
  9. With the treatment of thrombin for 16 hours at 24, the fusion protein was cut into 26 kd gst and 50 kd ri

    純化的融合蛋白於24經凝血酶作用16小時，可被切割成50kd的班和26kd的gst 。
  10. The purity was detected by sds - page, and only igg ' s heavy chain and light chain protein tope were showed up. the activity of igg was tested by technique of immune credeschs gold

    用sds - page進行igg的純度檢驗，電泳結果出現兩條蛋白帶，是igg的重鏈和輕鏈，說明純化的程度較高。
  11. Furthermore, the growth of escherichia coli tg1 and staphyloccus aureur atcc25923 were not under the influence of apss. the switching pulse induced a perceptible sustained transmembrane current on the planar lipid bilayer after unpurified aps added

    機理研究表明未經純化的aps作用於脂質平面膜，可以形成跨膜電流，提示活性成分可能通過改變脂質雙層膜通透性抗菌。
  12. The research consist of four parts. the first part is multiplication, purification and electron microscope examination of the avian encephalomyelitis virus. a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos. respectively. after incubation for 10 days, the urinay vesicle liquid was collected. making a comparison the size of the chickens embryos between the test group and the control group, the results showed that the size of the control group is bigger than that of the test group. purified virions were examined under the electron microscope, the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos. the second part was seguence analysis of the genome of the aev - nh937. nine pairs of primers were designed according to published calnek vaccine strain of aev

    本研究共分四個部分：第一部分為aev的增殖，純化和電鏡觀察，用1 : 5倍稀釋的aev - nh937株和陰性對照pbs分別經卵黃囊接種於6dspf雞胚，繼續孵化10d后，收集尿囊液。比較接種組和健康對照組雞胚的大小，結果顯示，健康對照組雞胚明顯大於接種組。分離、提純aev ，把純化的病毒在電鏡下觀察，證明確有大量aev病毒粒子存在，說明aev在雞胚中成功擴增；第二部分是aev - nh937基因組的序列測定工作。
  13. The substance with antibacteria action which had high purity was tested with escherichia coli, staphylococcus aureus, bacillus pyocyaneus and yeast by the antibacteria test to decide the minimal inhibitory concentration as 6. 37umol. l - 1, 3. 18umol. l - 1, 6. 37umol. l - 1, 6. 37umol. l - 1 25. 48umol. l - 1

    純化的抗菌活性物質通過對大腸桿菌、金黃色葡萄球菌、枯草桿菌、綠膿桿菌、酵母菌做抑菌試驗，最低抑菌濃度分別為6 . 37 mol
  14. The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter, length and shape being identical to that of muscle f - actins, which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ), and were identified as having a 9 nm diameter by negative staining, corresponding with that of the muscle f - actins ( 7 - 10 nm ). under polymerization conditions, his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin, that is, an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained. the critical concentration is 0. 75 umol / l near to that of chicken muscle actin ( 0. 56 umol / l ) under the same condition

    熒光標記結合熒光顯微觀察表明：從可溶性上清中純化的his - taggedpeac1 - gfp聚合形成的微絲不僅可以直接在熒光顯微鏡下觀察，也可被微絲的特異標記物鬼筆環肽所標記，而且其直徑、長度以及形態上與已知的聚合肌動蛋白熒光絲一致；電鏡負染的結果進一步證實其直徑為9nm ，與傳統微絲直徑相當（ 7 ? 10nm ） ；聚合曲線有明顯的停滯期，為典型的s型聚合曲線，聚合臨界濃度為0 . 75 mol l ，這一結果與已有報道相似。
  15. Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5 - dctp or cy3 - dctp into the reaction during reverse transcription of rna

    採用cartesian基因晶元列印儀，將純化的cdna片段列印在corning玻片表面，製作了酵母細胞基因表達譜晶元。
  16. But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation

    將表達產物破包涵體后經glutathinonesepharose4b柱親和層析分離， sds page顯示純化的蛋白質為一分子量60kd的單一蛋白帶。
  17. By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles

    對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。
  18. The study on the pureness method of the fat - soluble pigment of tagetes patula l

    天然孔雀草脂溶性色素純化的研究
  19. The gold particles and virus could be seen binded together in the electronic microscope, which indicated the activity of purified igg was high. clinical application of hyperimmunalserun was used to treat dogs with clinicalsigns compatible with canine distemper and parvovitus enteritis

    將純化的igg與提純的cdv 、 cav病毒反應後分別與膠體金標記的spa結合，在電鏡下可清楚地觀察到病毒與膠體金顆粒的結合，說明提取的igg的效價、活性較高。
  20. In 1994, li et al isolated agmatine from cow brain. like clonidine, agmatine is an endogenous agonist at imidazoline receptors ( ir ) and a noncatecholamine ligand at ( ai - adrenergic receptors ( ( x2 - ar )

    胍丁胺（ agmatine ）是1994年li等從牛腦中分離純化的一種內源性可樂定置換物質（ clonidinedisplacingsubstance ， cds ） ，它可能作為一種新型的神經遞質或調質。
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