細菌基因 的英文怎麼說
中文拼音 [xìjūnjīyīn]
細菌基因
英文
bacterial gene-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Bacterial transposons carry genes encoding antibiotic resistance.
細菌轉座子可以攜帶對抗生素的抗性基因。Isolate all grew well in the culture medium with initial ph 4 - 10, the optimal growth temperature range was from 28 to 30. 5 degree c. it grew well on the medium for fungi growth, such as pda medium and czpek medium etc, and also grew well on the cause ' s i medium and the non - nitrogen medium, but little growth on the luria bertani medium ( lb ) and beef extract peptone medium. it did not need special nutrition factors for growth, but source of the carbon was the key factor to growth, all of its nutrition needs were different from that of common bacteria
該菌在初始ph4 - 10的培養基中都能夠生長，生長最適溫度范圍為28 - 30 . 5 ，在pda 、查氏等真菌培養基中生長旺盛，在高氏1號和無氮源培養基中同樣生長良好，而在lb與牛肉膏蛋白腖等細菌培養基中生長很差，碳源是其生長的關鍵因子，這有別於一般細菌的營養需求。Hence, the widely quoted conclusion was reached that bacterial chemosynthesis provides the foundation for hydrothermal - vent food chains ? an exciting prospect because no other communities on earth are independent of photosynthesis
因此，曾有一度，被廣泛引用的結論是細菌化學合成提供了熱液噴口食物鏈的基礎? ?這是一個令人興奮的前景，因為地球上還沒有哪個群體能不依賴光合作用而生存。Key experiments that validate mathematical models are also discussed, as well as molecular, cellular, and developmental systems biology, bacterial chemotaxis, genetic oscillators, control theory and genetic networks, and gradient sensing systems
同時，亦會討論能驗證數學模型的重要實驗，同時也討論分子生物學、細胞生物學、發育系統生物學、細菌趨化性、基因震蕩器、控制理論、基因網路和梯度感應系統等。Computational analysis of the distribution of
序列在不同細菌基因組中分佈的分析Using pij903 bearing tsr gene as a vector, the two furthermost fragments of the cluster, 4. okb and 1. 5kb in size respectively, were inserted into it
Scp2 *的應用有兩個主要的限制，一是必須有被克隆基因簇的完整而詳細的遺傳學信息，二是要求它在被克隆基因簇的來源菌株中不能復制。A new transgenic system of gerbera mediated by agrobacterium tumefaciens
小細胞團為受體的根癌農桿菌介導的非洲菊基因轉化體系Dr venter reckons he will be able to synthesise a working bacterial genome from scratch within two years
文特爾博士估計，他能夠在兩年內從零開始，合成一個活細菌基因組。The strategy of bacterial genetic mutation and its application
細菌基因突變的策略及應用Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。Centrifuge for 10min at full speed in a tabletop microcentrifuge. the whitish pellet contains proteins and the bacterial genomic dna
用桌上型微量離心機以最高速離心10分鐘。白色沈澱物含有蛋白質與細菌基因組dna 。Further tests indicated that the bacteria have genes characteristic of ca - mrsa
進一步化驗顯示細菌具社區性抗藥性金黃葡萄球菌基因特徵。Hiroshi kiyono of the university of tokyo and his team experimented with genetic material from the bacterium responsible for cholera
東京大學的博史清野和他的研究組利用對能霍亂產生反應的細菌基因材料進行了實驗。Progress in genomic study and biochemical formation of magnetosome chains in magnetotactic bacteria
趨磁細菌基因組和磁小體成鏈機制研究進展Babies acquire their gut flora as they pass down the birth canal and take a gene - filled gulp of their mother ' s vaginal and faecal flora
嬰兒在經過產道並充分「攝取」富含細菌基因的母體陰道分泌物和糞便菌落之後，細菌便開始進入胃腸道。Sequencing of bacterial genes could also help researchers prospect for hitherto unknown chemicals made by our microbes that protect our health
細菌基因測序也能幫助研究者了解保護我們健康的由微生物構成的迄今尚未知的化合物。It is widely used in genetic research and genetic engineering as a repository for fragment of dna incorporated into plasmids in its cytoplasm
大腸桿菌細胞質中的dna片段可與質粒融合，並整合在細菌基因組中，所以被廣泛的用於基因研究和基因工程。The ability to synthesise huge screeds of dna at will means the genomes of viruses can be replicated already, and replicating those of bacteria is not far off
現在的科技可以隨意合成冗長的dna鏈，這意味著我們已經能夠復制病毒的基因組，而細菌基因組的復制也指日可待。分享友人