細菌接種法 的英文怎麼說

中文拼音 [xìjūnjiēzhǒngfǎ]
細菌接種法 英文
bacterial inoculaion
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 種 : 種名詞（姓氏） a surname
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 細菌 : germ; bacterium （pl. bacteria）; fungus （pl. fungi）
  • 接種 : [醫學] vaccinate; have an inoculation; inoculate
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Materials and methods : fecal, cloacal and tracheal swabs from different types of poultry were collected in 6 live - bird retail markets once a week. they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 " c for 72 hours. hi and nl assays were performed to detect the subtype of viruses if ha test were positive ( ad ^ 16 ) and contamination test were negative

    材料和方法：每星期收集一次標本，收集的標本常規處理， 9 - 11日齡雞胚尿囊腔接種， 35培養72小時，收取尿囊液，血凝陽性（ ad 16 ）且細菌培養陰性者- 70保存並進一步做hi ， ni實驗鑒定亞型。
  3. Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses

    沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
  4. En iso 9308 - 5 water quality - detection und enumeration of escherichia coli and coliform bacteria in surface and waste water - part 3 : miniaturized method most probable number by inoculation in liquid medium

    水質.表面和廢水中大腸桿菌和大腸菌類細菌的檢測和計數.第3部分:液體介質中的小型接種法
  5. The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target, lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt. the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1. 6 0. 1 104 the dosage of amd was lug / ml, and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution. the credibility of the established system was detected with rhtnfp developed by r & d

    為確定經上述步驟純化后得到的目的蛋自lt 27的生物活性，本研究以l929細胞為靶細胞、淋巴毒素國際標準品為參照，採用結晶紫染色法檢測經淋巴毒素處理后存活的細胞，對淋巴毒素生物活性測定的細胞接種濃度、淋巴毒素標準品板上稀釋的起始濃度和梯度稀釋的倍數、放線菌素d的使用劑量等進行條件實驗后，建立了人淋巴毒素生物活性測定方法。
  6. Because malaria parasites are far more complex than disease - causing viruses and bacteria for which vaccines now exist, malaria vaccines may never carry the same clout as, say, measles or polio shots, which protect more than 90 percent of recipients who complete all recommended doses

    因為瘧疾寄生蟲遠比致病的病毒與細菌復雜，而現有的疫苗也都是針對病毒與細菌，因此瘧疾疫苗也許永遠無法像麻疹或小兒麻痹疫苗那樣，只要打完所有建議的劑量后，接種的人90 %以上就可以受到保護。
  7. Design : viable mtb were detected in mesenteric lymph nodes, spleen and lungs at day 15, 30and 60 after intragastric administration of bcg to mice : the proliferation of t - lymphocyte with the stimulus of the purified protein derivative ( ppd ) was measured at day 60 and 90 after intragastric administration of bcg to mice ; the expression of interleukin - 2 ( il - 2 ) of t - lymphocyte in spleen was detected at day 60 and 90 after intragastric administration of bcg

    方法：在小鼠胃內接種bcg的第15 ， 30 ， 60天，檢測結核桿菌在小鼠體內臟器的定植；接種bcg的第60 ， 90天，檢測小鼠脾淋巴細胞受到ppd刺激后轉化試驗小鼠脾淋巴細胞受到ppd刺激后il - 2表達量。
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