組成型基因 的英文怎麼說
中文拼音 [zǔchéngxíngjīyīn]
組成型基因
英文
constitutive gene-
It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證By applying the th model theory to analyzing the cases of developed countries that mentioned in chapter 4, chapter 6 finds out the block gene and tendon compages that consists of the university - government - industry th. furthermore, it gives the pheno - types as some external expressions of the genotypes and the family - tree diagram constituted by these phenotypes. after a transition, it illuminates the scientometric, the webometric and the triple helix algorithm and their application
第六章運用th模型理論對第四章提供的案例挖掘梳理后，發現並給出了：構成大學?政府?產業三重螺旋體的完整板塊配基和鍵鏈組合，作為「基因型」外在表達的一些「現象型」屬類以及由它們所構成的圖式譜系。Despite several decades of trying, scientists have failed to fit einstein ' s general theory of relativity, which describes how gravity holds big objects together, with the quantum mechanics he pioneered, which describes the tiny fundamental particles of which matter consists and the forces by which they interact
盡管經歷了數十年的刻苦鉆研，科學家們仍然無法將愛因斯坦的廣義相對論與他所開拓過的量子理論統一在一起? ?這兩個理論前者是描述引力如何將大型天體維系在一起，後者則是描述組成物質的微小基礎粒子以及粒子之間的相互作用力。After entry of wto, there are still many maladjustments in guangxi ' s seed industry, such as the weak foundation of sees industry development ; the unformed market of the seed industry for fair competition ; small - scale seed enterprises ; no systematic connection among the cultivation, breeding and marketing ; lower qualification of staff who work in the seed industry and with weak sense of laws, and lack of the knowledge in operating experiences in the international market and trade etc. yet the un - efficiency system, unclear property right in enterprises, the lack of an effective mechanism to promote the rational use of resources in the seed industry and the lack of such concept as " the government creates environment and enterprises create fortune " are the deep - seated causes of the problems in guangxi ' s seed industry. therefore, the key points for promoting development of guangxi ' s seed industry under the wto framework are to focus on the promotion of the developing capability of seeds " integrated products, constantly deepen reforms, to adjust various relevant factors in the system of the seed industry which is inconsistent with each other, and to establish a new - pattern system with evident characteristics of the time spirit in order to meet the requirements of the market economy. hereinto, the specific strategies and measures for promotion of guangxi ' s seed industry development under the wto framework include kee ping up reform and innovation of the system of the seed industry, executing of non - nationalization reform in state - owned seed enterprises, formulating and executing relevant supporting policies, the improving the legal system in the seed industry, increasing public financial support on the seed industry, promoting the integration of cultivation, breeding and marketing, strengthening human resource development, developing the main body of the seed industry ' s market and making proper conditions for the functions of seed associations in the seed industry development etc
研究結果認為：發展種子產業應該把著眼點放在促進種子整體產品的開發上；種子產業的發展依賴于能充分發揮整體功能的新型種業體系的構建，而目前廣西種業體系中的品種選育、種子生產加工、種子經營以及政府管理、公共支持和社會服務六個主要組成部分都存在明顯的缺陷與不足；廣西種子企業綜合競爭力總體處于較弱水平；面對wto ，廣西種子產業仍有諸多的不適應，突出表現在產業發展基礎薄弱、尚未形成可以公平競爭的種業市場、種子企業規模小、育繁銷脫節、種業人才素質不高、種子企業法律意識淡薄、國際市場運作經驗和國際貿易知識不足等多個方面，而體制不順、企業產權不明晰、缺乏促進種業資源合理流動的有效機制以及「政府創造環境，企業創造財富」的正確理念正是導致目前廣西種子產業不能適應入世需要的深層次原因；因此，以提升種子整體產品開發能力為核心，不斷深化改革，調整種業體系中不相協調的各有關因素，構建起符合市場經濟體制要求的具有鮮明時代特徵的新型種業體系，是wto框架下加快廣西種子產業發展必須堅持的指導思想；其中，加快種業體制改革和創新、實施對國有種子企業的非國有化改造、制訂落實有關扶持政策、完善種子法律法規體系、加大公共財政對種子產業的支持力度、推進育繁銷一體化的形成、加強人才培養與引進、壯大種業市場主體、實行重點突破戰略、發揮種子行業協會作用等等，都是wto框架下加快廣西種子產業發展應該採取的具體對策措施。Guan xiaohong ( 1991 ) established a cell line of the monoclonal anti - idiotypic antibody ( anti - id ) np30 of schistosoma - 6 - japonicum, whose isotype was identified to be igm and which was testified to be internal image of gaa
分別以日本血吸蟲單克隆抗獨特型抗體wbo的雜交瘤細胞株總基因組dna和其提取rna進行rticr合成的cdna第一鏈為模板，擴增v 。 、 v 。Using the net assets per capital, the investment return rate, the t - m model, the h - m model, the single factor evaluating model which consists of the treynor index, the jensen index, the sharpe index and the square m index, we evaluate the performance of the twelve mutual funds. and we come to the following conclusions : ( 1 ) after the modification of the risk factor, our mutual funds in the recent one year outguess the market ; ( 2 ) better performance comes from the aid of the government, the improvement of the investment environment and the hard, smart work of the managers especially in the way of selecting some securities in the capital market. ( 3 ) though we make progress, there are still many problems which prevent the further development of our mutual funds such as the devise of the management fee and the characteristics of different funds, all of them divided into the subjective ones and the objective ones
通過使用投資基金單位凈資產和投資收益率指標、單因素整體績效評估模型，包括treynor指數、 jensen指數、 sharpe指數和業績的m ~ 2測度以及t - m 、 h - m模型對12隻樣本基金進行實證研究，實證研究表明： （ 1 ）經過風險調整后，在最近的一年中，我國證券投資基金的業績總體上優於市場基準組合； （ 2 ）基金業績的提高得益於管理層的重視、投資環境的改善和基金經理的經營，而基金經理的良好業績是通過一定的證券選擇來獲得的； （ 3 ）已成為證券市場上舉足輕重力量的基金在發展過程中雖然取得了一定的成績但其進一步發展還面臨著許多問題，有主觀存在的諸如管理費率的設定、基金風格方面的問題等等，也有客觀存在的諸如證券市場現階段的不完善等等，所以，我們應該抓住《證券投資基金法》問世帶給基金業發展的契機，大力促進證券投資基金規范發展，採取各種措施做大、做優和做強基金業。Compared with non - passerine, they appeared longer syllable length, higher basic frequency and more viable frequency ranges. though the amplitude modulation properties enhanced their vocal production, the type of syllable is still stable and less of variety. so the phrase consisted of syllables are comparatively few and changes of the tone are relatively less
且表現出很強的頻率調制特性和特有的幅度調制特性，相對的音節時程比較長，基頻較高且頻率變化范圍較大，所特有的幅度調制特性更增加了其控聲的能力，但其音節的種類仍很單一，缺乏變化，由音節所組成的句型較少，音調變化幅度不大，因此，其叫聲質量盡管較非鳴禽有所改善，但相對鳴禽而言卻又遠遠不如。The recombinant b. thuringiensis subsp. israelensis b - pmpx2 can produce a rhombus crystalline inclusion body during its sporulation sds - page analysis proved that cytlaa had overexpressed during the sporulation of the recombinant. it was found that b - pmpx2 strain remained stable toxicity, whatever during vegetative phase or sporulation phase, which was similar to the expression of mtxl gene in the strain of protease ( s ) - deficient b. sphaericus
同時，將來源於蘇雲金芽孢桿菌以色列亞種（ b . thuringiensissubsp . israelensis ， b . t . i ）的cytlaa基因和p20伴侶蛋白基因克隆連接到質粒pmt9中，並轉化到蘇雲金芽孢桿菌無晶體型中得到重組轉化子b - pmpx2 ，電鏡觀察發現重組轉化子b - pmpx2形成一菱形晶體。Thereafter, the applications of go sequences in single - cell s - cdma systems are discussed in this thesis. in additive white gaussion noise ( awgn ) channel, interference components in s - cdma systems accommodating more users than the spreading factor is analyzed based on the theoretical bound of the periodic correlation mean square property. then two interference - suppressed s - cdma system models of augmented capacity are proposed, along with the analysis and simulation results
在加性白高斯噪聲（ awgn ）通道條件下，以序列集周期相關均方特性理論界為基礎，對序列個數大於系統擴頻因子的s - cdma系統干擾組成進行了分析，並基於截短wh序列提出了兩種高容量低干擾s - cdma系統模型，給出了分析和模擬結果。Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively
用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。This gene, artificially put under the control of camv 35s, was introduced into tobacco with the aid of agrobacterium, and the transgenic plants obtained were identified by gus staining, pcr and southern blot analysis
將arge與組成型啟動子camv35s相連，構建植物表達載體，通過農桿菌介導轉化煙草。經過gus染色、 pcr及southern鑒定獲得了轉基因植株。As both the recombinant plasmids and the host dh5a had no laciq gene which encode lac represser, the trc promoter was not repressed by lac represser and always active. therefore the afpga could be constitutively expressed without iptg induction in the host dh5. in the shaking flask, the optimum ph and temperature for afpga expression was 7. 0 and 30
兩重組質粒再轉化宿主菌dh5 ，由於重組質粒和宿主菌dh5均不含laci ~ q調控基因，重組菌不產生阻遏蛋白，控製表達的trc啟動子始終處于「開」狀態，因而所得重組菌dh5 pkkfpga和dh5 psmlfpga不需誘導便能組成型表達pga 。In this dissertation, influences of various factors on plant regeneration and agrobacterium - mediated transformation of tall fescue embryogenic calli were systematically studied, and thereafter, stress tolerance - related cbf1 gene guided by constituent promoter camv 35s was incorporated into genome of this grass to obtain transgenic plants with increased stress tolerances
本論文在對高羊茅胚性愈傷組織植株再生與農桿菌介導遺傳轉化的多種影響因素進行系統研究的基礎上，將組成型表達啟動子camv35s引導的耐逆相關cbf1基因導入該草種的基因組，獲得耐逆性增強的轉基因植株。However, so far only the results obtained by whitelaw et al ( 2002 ) concerning leetrl gene were partly consistent with the negative model and partly against it. obviously, the action models for these ethylene receptors are far from being clearly understood
目前已經從模式植物番茄中分離了6個受體基因（ leetr1 6 ） ，對它們的基因和蛋白結構特徵、表達特性等已經有較多的研究，但是對組成型表達受體基因在乙烯受體系統中的功能研究較少。The plant expression vector pbin19 - rok219 was constructed, which contains cauliflower mosaic virus ( camv ) 35s promoter and noster. introduction of ibdv vp2 gene under control of camv 35s promoter resulted in the construction of binary expression vector pbr - vp2. then the vp2 gene was in the left and right border regions, which denote the limits of the dna that is integrated into the plant genomic dna via agrobacterium fwme / ac / ms - mediated transformation
構建了表達載體pbin19 - rok219 ，以ibdv甘肅天水株的抗原基因vp2為目的基因，將vp2基因置於植物組成型表達啟動子camv35s之下，構建了一個ibdvvp2基因的植物表達載體pbr - vp2 ，這樣使vp2基因位於農桿菌t - dna的左右邊界重復序列之間。We have created transgenic tobacco plants in which expression of the atnced3 coding region is under strong constitutive 35s cauliflower mosaic virus ( 35s ) promoter, stress - inducible rd29a promoter or stomotal - specific kst1 promoter by agrobacterium tumefaciens - mediated transformation
構建了組成型35s 、誘導型rd29a 、氣孔特異表達性kst1啟動子驅動atnced _ 3基因表達的真核表達載體，通過農桿菌介導的葉盤法轉化煙草，得到了轉基因植株。The result of rt - pcr of transgenic plants showed : in transcriptional level, vp7 gene was expressed in leaves and fruits of 4 transgenic tomato plants under control of camv35s promoter ; vp7 gene was expressed in the tomato fruits not in the leaves of 3 transgenic tomato plants under control of fruit - specific tfp promoter
Rt - pcr分析證明，在組成型啟動子camv35s控制下的5株轉基因植株的葉片和果實中，有4株vp7基因在轉錄水平上表達；而在果實特異表達啟動子tfp控制下的3株轉基因植株， vp7基因僅在果實中獲得轉錄水平上表達。The characteristics of various cis - regulatory elements of plant promoters, such as tata - box, transcription start point and upstream elements, and different types of the plant promoter, such as constructive promoter, tissue - specific promoter and inducible promoter, as well as bi - direction promoter, alternative promoter and linked promoter will be described or analyzed in this present paper
並著重從組成型、組織特異型和誘導型啟動子3個方面介紹了其結構特徵、功能，以及它們在植物基因工程中的應用和研究進展，簡述了雙向啟動子、可變啟動子和串聯啟動子的研究情況，提出植物啟動子研究中存在的問題與展望。The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。This reduced haplotype diversity is a result of the recent expansion of human populations from a small number of shared ancestors from africa, and is most marked in genomic segments called haplotype blocks, separated by gaps where genetic material has been historically reshuffled
單體型變化減少是因為近代人口的祖先都是源自少數非洲人，這可以在基因組分段的單體型區段清楚見到，由於進化演變關系造成之基因物質之間出現隔閡。分享友人