缺失重組 的英文怎麼說

中文拼音 [quēshīzhòngzǔ]
缺失重組 英文
deletional recombination
  • 缺 : Ⅰ動詞1 （缺乏; 短少） be short of; lack 2 （殘缺） be missing; be incomplete 3 （該到而未到） be ...
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 缺失 : hiatus; deficiency; [遺傳學] deletion
  • 重組 : bpr
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank

    以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. The analysis of five typical cases, which are miketer ' s cheating in listing, the artificial accounting by yinguangxia, the deceitful reorganization and inside story of yian technology, the chaos of creditor ' s rights and liabilities of jiuzhou stock company and the misappropriation of stock company ' s benefits by meiera stockholder, are listed from chapter 2 to chapter 6 respectively. the operation report, pronunciamentos and the medium and research institutions " comments on those companies are referred in this research, and the introduction of those companies, the facts of the credibility deficiency and the comments of those facts are also included in this analysis

    第二章至第六章分別對麥科特欺騙上市案例、銀廣夏假賬案例、億安科技虛假重組及內幕交易案例、九州股份債權和債務混亂案例和美爾雅大股東侵佔股份公司利益案例進行分析，案例研究參閱了幾家上市公司歷年業績報告、公告書、有關媒體及研究機構對公司及事件的評論等材料，分析過程包括這幾家上市公司基本情況介紹、信用缺失的主要事實以及對案例的評述等。
  5. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  6. Deficiency of apoe may promote to produce and develop atherosclerotic lessions. the apoe gene - targeted mice will result in marked regression of both early and advanced atherosclerotic lesions by injected apoe recombinant protein, or by transfected adviral vector with apoe cdna to express human apoe transgene in liver, or by transplantation of bone marrow with normal rat apoe gene. this demonstrates that apoe gene and its expressing product can inhibit progression of atherogenesis. apoe3 has a more effective prevention from as than apoe2 and apoe4

    Apoe的缺失可促進動脈粥樣硬化的發生發展，給apoe基因敲除鼠反復注射apoe重組蛋白、在肝組織中用腺病毒載體表達apoe蛋白、移植帶有正常apoe基因的小鼠骨髓，都能使apoe基因敲除鼠的動脈粥樣硬化得到回復，表明apoe基因及其表達產物對動脈粥樣硬化的發生具有抑制作用， apoe _ 3對動脈硬化的阻抑作用要比apoe _ 2和apoe _ 4都明顯。
  7. The result shows that a vvibdv strain was obtained, the above work lay a important role for further studying on the molecular biological mechanism of antigenic drift and virulence variation of ibdv, molecular epedimiology, it also provided the basis for recombinant and gene deleted vaccine of ibdv

    本實驗可以幫助我們進一步探討ibdv抗原性漂移和毒力變化的分子生物學機制，追溯ibdv的起源，理解病毒的傳播方式。同時也為研製開發基因重組疫苗和缺失疫苗打下一定的基礎。
  8. Chromosome karyotypintg. the classical method in the technology of chromosome genetic analysis, is one of the important means in genetic research and supplementary clinical diagnosis. and it is then key index to analyze chromosome translocation or deficiency, and diagnosis of a variety of genetic diseases. the goal of chromosome analysis is to relate deviations from normal structure to biological or clinical effects

    染色體核型分析，染色體遺傳分析技術的經典方法，是遺傳學科學研究和輔助臨床診斷的重要手段之一，是分析染色體易位，缺失，診斷各種遺傳病變的關鍵指標，染色體分析的目的就是要確定細胞或個體的染色體組成，尤其是要將其與正常結構間的偏差和生理的或臨床疾病關聯起來。
  9. In part in, the author studies the obligation - blinding mechanism to the trustees, which is the core of governance structure of fund, pointing out that one of the main reason for the serious abnormality of current funds is the lacking of duty performance of the trustees. though it is hand to set up an effective obligation - binding mechanism to the trustees under the chinese current legal system, we can learn from britain and the u ? s ? a. in part iv, the author studies the mechanism of balance inside the governance structure of funds, that is the supervision mechanism of trustors and the balance mechanism of fund - holders, then puts forward that strengthening the balance mechanism inside the funds is an important step to establish an effective governance structure of found ; further more, to deal with the problems in the two mechanisms the author makes some suggestion about perfecting the mechanism of balance inside the fund in china

    本文共分四個部分，第一部分通過對信託制基金本質和特點的分析，指出了架構信託制基金治理結構的必要性，並闡述了架構我國基金治理結構應遵循的基本原則；第二部分對架構信託制基金治理結構的基礎，也就是信託制基金的組織結構進行了研究，認為我國基金組織結構存在較大缺陷，但現存基金組織結構的立法模式也不適宜我國照搬照抄，而自益信託、共同信託才是我國信託制基金組織結構的應然選擇；第三部分對基金治理結構的核心問題，即基金管理人的義務約束機制進行了研究，指出：我國目前存在的基金行為異化現象嚴重的主要根源之一就是基金管理人義務的缺失；但在我國現行法律制度框架內無法架構起有效規制管理人的義務機制，而英美法對基金管理人的受信人義務的規定值得我們借鑒；第四部分對基金治理結構的內部監控機制，即基金託管人的監督機制和基金持有人的制衡機制進行了研究，指出：強化基金內部監控機制是架構有效的基金治理結構的重要一環；並針對我國這方面機制存在的問題提出了完善我國基金內部監控機制的建議。
  10. The purpose of this study is to construct a recombinant pseudorabies virus expressing ha gene of swine influenza virus and to develop specific diagnosis for differentiating the recombinant virus immunized animals and those naturally infected with prv or sfv

    本試驗的目的是以ge基因缺失的prv為載體構建可以表達sivha基因的重組病毒疫苗用於預防這兩種病，同時，基於此重組病毒的特點，建立可以區分此重組病毒疫苗和prv及siv自然感染的鑒別診斷方法。
  11. Though shanghai bar association was one of the best organizations in shanghai and had great influence in associations of the whole nation, lacking functions of credential examination and punishment were its tremendous defect

    雖然上海律師公會是上海組織得最好的團體之一，並在全國律師公會中具有重大影響，但資格審核功能與懲戒功能的缺失卻是其重大缺陷。
  12. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  13. A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss

    採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。
  14. A deletion recombinant virus ( hasnpva132 ) of hal32 was generated by homologous recombination in e. coli. electron microscope pictures revealed the deletion virus could replicate in hzaml cells, which indicates hal32 is not essential for the replication of hasnpv

    通過在大腸桿菌內同源重組構建得到orf132的缺失重組病毒（ hasnpv凸132 ） ，轉染成功后的電鏡切片表明， hasnpv凸132能夠在hzami細胞內正常復制、增殖。
  15. Not seem as the traditional research route this paper in terms of maxism and the evolutionism theory to study the absorptive capability attribute and impress along with the knowledge innovation, to study knowledge innovation chain structure and contain among the different course compare with the lack and narrow sense and broad sense state of absorptive capability, accompany with market and state innovation system working. it reaches conclusion as follow : 1. the absorptive capability is substantial which make up of core competence and take a important role for enterprises to capture overflow knowledge of competitor and common knowledge in society, to deepen inner r & d activity, to build up the utility of organization and information intercourse

    本文以馬克思主義唯物史觀和辨證分析理論為指導，運用企業能力進化論和企業發展演進的分析方法，圍繞隨著企業知識創新在各個階段的不斷深化所帶來企業吸納能力的形成與增進，探討了吸納能力范疇及其在不同創新過程中的特徵；提出了企業知識創新鏈結構，分析了吸納能力缺失、狹義吸納能力和廣義吸納能力增進的不同內涵；知識創新市場機制和國家創新體系對企業吸納能力增進的環境效應等，得出以下結論： 1 、吸納能力對于企業有效地獲取外界公共知識和競爭對手溢出知識、深化r & d活動、增強組織結構的有效性和信息交流的充分性等具有十分重要的意義。
  16. In default of monetary incentives and promotion incentives, it ' s very important to intensify the construction of organizational culture

    在貨幣報酬激勵和職務晉升報酬激勵缺失的條件下，加強組織文化建設意義重大。
  17. " tax - synergy " may affect the financial decisions of the firms so that investments are undertaken, which would not have been made without taxation. if asset reorganizations of other companies are favored, for example, the tax system is not neutral in this respect. one may call this the absence of " reorganization - neutrality " in the tax system

    反對給予稅收激勵政策的學者則認為，資產重組中的要約溢價主要來源於稅收節約，稅收引致的財務協同效應對公司投資決策產生影響，這種稅制的「缺乏中性」將導致私人收益和社會收益的偏離，產生效率損失。
  18. To confirm that the histamine receptor connects the drug, ampk activity and appetite, the team gave clozapine to mice genetically engineered without a histamine receptor

    為證實組胺受體和藥物、 ampk活性、和食慾相關，研究組給經遺傳重組后缺失去組胺受體的小鼠氯氮平藥物。
  19. Many streptomyces species exhibit a very high degree of genetic instability which is usually manifested as genomic rearrangement such as large deletion, and high - level dna amplification of those sequences flanking the deletion

    許多鏈黴菌表現出高度的遺傳不穩定性，通常為大片段缺失或基因組重排，以及缺失區域兩側序列的高水平dna擴增。
  20. The groc also shepherds a workshop series designed to bring multiple ggf working groups and research groups together to look at overlaps and missing pieces

    Groc也負責一項工作室計劃，目的是將多個ggf工作組和研究組結合起來，看看是否有重復和缺失的方面。
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