缺氨酸突變型 的英文怎麼說
中文拼音 [quēānsuāntūbiànxíng]
缺氨酸突變型
英文
glutamic acid mutant-
The comparative analysis of sequences indicated that the sequences of meq in different pathotypes are relatively conserved and the homology of the amino acid sequences is very high. the significant differences include two mutations in both mdv - 1 vaccine strains cvi988 / rispens and 814 strain : the deletion of a proline ( no. l93aa ), and this mutation is just exactly located in a 15 - amino - acid ( eelcaqlcstppppi ) repeat sequence within the c - terminal transactivation domain of meq protein ; and a point mutation with a shift from alanine ( a ) of all virulent strains to serine ( s ) was occurred on the no. 71 aa
結果發現， mdv不同致病型的meq基因序列相對比較保守，它們相互間核苷酸和氨基酸序列的同源性均很高；但是，與所有七個致瘤性的mdv毒株相比，二個型弱毒疫苗cv1988 rispens株和814株均出現了兩個特徵性的突變：即第194位的p缺失性突變和第71位氨基酸由a變成了s的位點突變；缺失性突變恰恰位於meq基因中轉錄激活域內的一個多脯氨酸的重復序列（ pppp ）之中。A combined metabolic mutated strain ( ys - c ) was get by traditional filter method from corynebacterium glutamicum ( ys ). this mutant is threonine auxotrop, aec and ahv resistant, and it can grow in the medium which contants sca as the only source of carbon, and the mutant can endure hight leveal of leu. the mutanted strain can concentrate lysine. the concentration of lysine leveal was 1. 20g / l
運用傳統育種手段篩選到谷氨酸棒桿ys的多重代謝突變組合菌株ys - c 。該菌株為蘇氨酸營養缺陷型，具有對賴氨酸的結構類似物aec和ahv的抗性，並且能以琥珀酸為唯一碳源生長，能夠耐受高濃度亮氨酸。The orf1 protein of senv - h12 - 1 isolate was consisted of 675 amino acids and 92 amino - terminal amino acids less than senv - h ( ax025838 ), it had insertion in two sites also. the orf1 protein of senv - h 12 - 2 isolate was 7 amino acids less than senv - h ( ax025838 ) because of deletion. because the first start code was mutated, the orf2 protein of senv - h 12 - 2 isolate started from the second atg code, so that it was 7 amino acids less than senv - h ( ax025838 ) from its n - terminus
兩個senv - h亞型分離株的情況與已發表的博士學位論文: sen病毒分子病毒學和流行病學研究對sen琴h （ ax025838 ）株分析的結果有所不同: senv - h12一1分離株的orfi僅由675個氨基酸組成，比senv - h （ axo25838 ）的orfi少n一末端的92個氨基酸，此外在2個位點上還存在插入: senv - h12一2分離株在一些位點上的缺失造成o盯1蛋白比senv - h （ axo25838 ）少7個氨基酸，由於第一個起始密碼子發生突變，採用位於下游的第二個起始密碼子使得o即2蛋白比senv - - h （ axo25838 ）少n一末端的7個氨基酸。
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