胚細胞中毒 的英文怎麼說
中文拼音 [pēixìbāozhōngdú]
胚細胞中毒
英文
blastotoxy- 胚 : 名詞[生物學] (初期發育的生物體) embryo
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞,從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞,並且這種凝集可以被特異性抗血清所抑制。Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks
生物學特性:雞胚尿囊液經離心、磷鎢酸負染后,電鏡觀察該病毒為典型的冠狀病毒;該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用,當繼代到第5代后,胚體嚴重病變;病毒在雞胚中隨著接種時間的延長,其效價增高, 96h可達到48h的2倍;該毒株可在cef上生長,但不能形成明顯的蝕斑;經1胰酶處理后可凝集雞紅細胞;雞胚的第四代尿囊液病毒回歸動物體,病死雞腎臟呈典型的花斑腎,腺胃則未見肉眼可見的病變。The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時,還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試,並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學,細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞,但其遺傳學和細胞生物學背景知之甚少。The propagules of b. gymnorrhiza and a. corniculatum undergo some growth before becoming detached from the parent plant. they accumulated large amounts of ions during the development while the level of ion concentrations are consistently lower in their embryos compared to leaves of parent plant. before detachment, the growth of hypocotyls were reduced, the water content declined, while the osmotic potential was approaching to or more negative than that of the solution on which their parent plant vegetating
另一方面維持較大比例的有機滲透調節劑,控制細胞液中鹽離子的水平以減少代謝毒害;木欖成熟胚軸的密度接近或低於生境中海水的密度,適于隨水漂浮和傳播;胚軸內離子濃度呈下降趨勢,但最終以達到和母樹生境中的離子濃度水平相一致為目標。In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures
幼雞感染該病毒后,引起麻痹、頭頸震顫甚至共濟失調,而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響,鎂離子可增強病毒對熱的穩定性,病毒的浮密度為1 . 31g ml ,直徑為22 - 30nm ,該病毒主要在雞胚中增殖,在大多數細胞培養物中不生長。In the present thesis, it is mainly to express the human angiostatin ( kl - 3 ) in bombyx mori cells ( bmn ) and larvae of silkworms with baculovims expression vector system ( bevs ), to investigate the effect of human angiostatin ( kl - 3 ) on cell apoptosis in endothelial cells, and on vascular growth in chick embryo, and to elucidate cooperation between angiostatin and endostatin
因此,在本實驗中我們用家蠶桿狀病毒表達系統表達了人血管抑素( k1 - 3 ) ,並研究了其對內皮細胞凋亡和雞胚新生血管生長的影響,闡明了其與內皮抑素的協同作用。The results revealed that antigenic drift had occurred in virus strains of the same h9n2 subtype in the north of henan province during 1998 - 2002. h9n2 subtype aivs were stable from 1998 to 1999, since the mutual protection between 98a5 and 99s approached to 100 %, which was consistent with results in hi and vn tests
1998年和1999年h _ 9n _ 2亞型禽流感病毒較穩定, 98a5和99s毒株間的保護力接近100 ,在hi 、雞胚中和、細胞中和試驗結果的相關性均在0 . 74以上,表明兩毒株間的抗原性相近。The use of retrovirus - mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell growth
運用反轉錄病毒介雜的插入突變技術,在脊椎動物斑馬魚中已經獲得了許多影響胚胎發育和細胞生長過程的突變體,並找到了對應的基因。分享友人