胞質抗體 的英文怎麼說
中文拼音 [bāozhíkàngtǐ]
胞質抗體
英文
cytoplasmic antibody-
With the polyclonal antiserum against testin and actn1, and the monoclonal antibody against ap - 2a, the expression of testin, actn1 and ap - 2a in hela cell was determined
用制備所得的抗gst - testin血清和抗gst - actn血清及ap - 2的單克隆抗體分別檢測了這三種蛋白質在hela細胞中的表達。Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。The purity of eorabl protein, cleaved gst - eorabl protein with thrombin protease, reached 95 percent
這為gtpase活性的深入研究，抗體的制備及細胞內定位準備了前提物質。Vasculitis associated with propylthiouracil - induced antineutrophil cytoplasmic antibody is not drug induced lupus
丙基硫氧嘧啶引起抗中性粒細胞胞質抗體相關小血管炎並非藥物性狼瘡After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Nutrilite protein is naturally derived from soya beans and offers nine essential amino acids necessary for cell maintenance, antibody production and immunity building
健爾力蛋白質粉提煉自純天然大豆，提供9種人體必需的胺基酸，能修補細胞，製造抗體，有助增強抵抗力。Nutrilite protein is naturally derived from soya beans and offers nine essential amino acids which are necessary for cell maintenance, the production of antibodies and to build up the body ' s immunity
健爾力蛋白質粉提煉自純天然大豆，提供9種人體必需的氨基酸，能修補細胞，製造抗體，有助增強抵抗力。Anti - if - protein antibodies were used as probes for immuno - fluorescence, and the reactions of them with different parts of the cell were detected, which suggested the possibility of the existence of the intermediate - like filaments in the cytoplasm. those proteins homologous to the antibodies distributed regularly in the protoplasm. to characterize the corresponding proteins, sds - page and immunoblots were utilized. the 21, 23, 33 and 68kd proteins were distinguished among the diverse protein constituents of the cell. some of these proteins also showed the cross - reactivities with anti - if - proteins antibodies derived from higher organisms. these two evidences both contributed to the homology of some proteins in
以抗中間纖維蛋白抗體作為探針進行免疫熒光實驗，得到細胞內不同部位的陽性反應，暗示原生質中可能存在類中間纖維。這些同源蛋白的胞內分佈具有一定的規律性。進一步採用sds - page和免疫印跡技術研究它們的生化性質，發現4種主要蛋白明顯有別于胞內其他蛋白組分。Enhancement of glioma cell apoptosis induced by anti - human dr5 dr4 monoclonal antibodies by sub - toxic dose of doxorubicin in human
5抗體誘導的神經膠質瘤細胞凋亡的研究By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。The who classification states that acute lymphocytic leukemia ( all ) should be classified by the pattern of reactivity of cell to a panel of lineage - associated antibodies and, where possible, genetic abnormalities. the expression of cd antigens on leukocytes is currently determined by flow cytimertry, which is expensive and labor - intensive, requiring 5 - 20ul quantities of fluores - cently labeled antibodies and allowing concurrent analysis for a limited number of cd antigens, usually three to four. from clinical point of view, an efficient method is required for analysis of a large number of samples in a single experiment
我們設計了一種新型的細胞晶元，其原理是充分利用玻片的硬度高，透明性好的特點，應用細胞膜表面不同的抗原物質，與包被在玻片上的不同抗體發生特異性結合，通過一次實驗可以將被測細胞懸液中不同膜表型的細胞分離、固定在同一張玻片的不同特定區域，保持了細胞的完整性及活性，又可通過簡單的操作一次將多細胞懸液進行免疫分群。These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr. it seems that the increased 1, 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n, while the decrease of this branch may prevent these processes
用特異性抗體結合westemblot結果發現，正義或反義gnt一vcdna的轉染並不引起pkb 、 p44 / 42mapk和mek蛋白質表達的變化，而gntv一s / h 」 21細胞pkbt308 、 5473位點磷酸化和免疫沉澱pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化為指標的pkb的活性都較mock細胞增加， gntv一as / h7721細胞中這些指標的變化則相反。Pre - clinical studies showed that needleless injected fluids follow the path of least resistance and does not pass into the substance of bone, media of blood vessels or into the fibre substance of the nerve trunks
臨床研究顯示，無針美槊療法所注入的液體不會滲入骨頭、血管或神經內，它會沿著最少抗體的路徑進入肌膚組織，並且加速皮膚細胞的新陳代謝，讓雞尾酒中胚層綜合物質更容易於肌膚所吸收。Thin sections of host leaf cells infected by bbwv - 2 isolate b935, which were gold - labeled by antibodies of bbwv - 2 coat protein ( cp ) and vp37, respectively, were prepared to elucidate the locations of vp37 in cell and possible function of vp37 and cp in cell to cell movement. observation in electron microscope showed that virus particles were presented not only in cytoplasma but also in chloroplast, while vp37 was existed only in cytoplasma and associated with tubular structure through the cell wall
為研究vp37在寄主細胞中的作用機制及其在細胞中的分佈，通過膠體金間接標記6his - vp37兔抗血清，同時還標記了病毒的外殼蛋白單克隆抗體，對bbwv - 2分離物b935感染的病葉超薄切片的電子顯微鏡觀察發現：病毒粒子除了聚集在胞質中，還存在於寄主的葉綠體內； vp37蛋白能在細胞壁上形成管狀結構，在胞質中亦有分佈。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。These proteins are ubiquitous in the plant kingdom where they form a multiple genetic family. they have been suggested to be involved in dif ferent aspects of plant physiology and cell biology through their ability to bind and / or carry lipophilic compounds, including the formation of cutin by transporting the hydrophobic cutin monomers to the apoplast and the defence of plants against pathogens as antimicrobial agents and in flowering
此外nsltp在植物體內廣泛存在並由一個基因家族編碼，由於它能結合和轉運脂類物質，因此推測它可能參與植物體內許多不同的生理過程，如轉運蠟質的角質單體到表皮細胞外側，作為抑菌蛋白抵抗病原菌的入侵以及花粉和柱頭的識別等。The recombinant was identified by dual enzyme digestion and the direction of cd40 / pcdna3 was analysed with t7 primer. after being packed by lipofectaminetm2000, the recombinant was transfected into b lymphocytes. cd40 expression on membrane, cell proliferation and antibodies concentration were detected with flowcytometry, mtt colorimety assay and el1sa, respectively
以脂質體為介質瞬時轉染健康人及sle患者b細胞系，利用流式細胞技術檢測膜cd40的表達情況，並利用mtt比色法檢測細胞的增殖能力， elisa法檢測培養液的ig濃度，以研究b細胞在cd40被抑制以後增殖能力、抗體分泌的改變。The surface - modified magnetic nanoparticles have been widely used in biological and biomedical areas such as immobilization of biomolecules ; separation of metal ions, cells, proteins, dna ; immunoassay, biochemical analysis and magnetic resonance imaging ( mri ) probes
其中的殼結構既具有生物適應性，又具有可鍵合生物分子如細胞、蛋白質、酶、抗體和核酸的活性基團，而核具有磁性特性。分享友人