胞質粒 的英文怎麼說
中文拼音 [bāozhílì]
胞質粒
英文
cystomyxo-adenoma albuminous granule-
The changes of the expression of gfp in biu - 87 cell that induced by the aconitine and hab toxins, gtx were detected with fluorescence microscope, and quantitatively measured with image - pro plus software
經誘導,抗性細胞發出較強的綠色熒光,表明重組質粒pegfp - c - fos在biu - 87細胞中成功表達。First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。Stable suppression of afp gel expression by rnai in smmc - 7721 cells
表達質粒穩定轉染肝癌細胞克隆的構建The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量,我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列,目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上,構建ppic9k - fl表達質粒。The cytoplasm between the desmosomal attachments shrinks.
橋粒間的細胞質皺縮。A small region of cytoplasm adjacent to the nucleus that contains the centrioles and serves to organize microtubules
靠近細胞核的一個細胞質小區域,含有細胞中心粒,為形成微管服務。In submicroscope, respiratory cell is flat, there are microvillus on it s surface, and lots of chondriosome in the cytoplasm and some gdgi 10 complex distribute near the nuclear. the other cell organ has not been found
電鏡下呼吸細胞扁平,表面有微絨毛,胞質中分佈有一定數量的線粒體,在核旁分佈有高爾基復合體,其它細胞器則少見。Secretic granules in b cell are fairly big, cores are various. there is fairly big gap between the core and the limiting membrane ; the center of secretic granules in a cell exist compact core and there is no gap between the core and the limiting membrane ; secretic granules in d cell are too much, electron density of granules is lower, and there is no gap between the core and the limiting membrane, what ' s more, fairly big chondriosome can be seen in the cell
B細胞的分泌顆王曉麗尼羅羅非魚胰島顯微和亞顯微結構的研究13粒較大,芯的形態多樣,電子密度差異大,芯與界膜之間常有較大的空隙; a細胞分泌顆粒形態不規則,芯的大小不等,界膜與芯之間無空隙; d細胞分泌顆粒較多,芯的電子密度較低,界膜與芯之間無空隙,此外,胞質內可見較大的線粒體。A lot of vacuolus occurred in the cytoplasm of the cells. the nuclear membranes and cristae in mitochondria were disintegrated partially
中腸細胞內含有鉛顆粒,細胞質出現空泡化,核膜和線粒體內嵴部分解體。There were copper granules in the cells of midgut. a lot of vacuolus occurred in the cytoplasm of the cells. the cristae in mitochondria were disintegrated partially and copper granules were absorbed into mitochondria
中腸細胞內也分佈有銅顆粒,細胞質出現空泡化;線粒體內嵴部分解體,並有銅顆粒附著在線粒體上。In spermatogenous cell, we could find dilated cytolemma, dilated nuclear membrane and fused crista of mitochondria. most of the sperm ' s cellular membrane dissolved and broke down into pieces. the central granule and mitochondrion showed little dissolving
久效磷可以使金魚精子細胞質膜溶解,出現間斷性斷裂;有的精子頭部變形,頂部出現凹陷;少量精子頸部中心粒復合體溶解;精子頸部線粒體有溶解現象,精子尾部的膜結構斷裂。At low concentration of zn, the changes in ultrastructure were nuclei deformation, chloroplast swelling and disorder of thylakoid arrangement ; serious damages in ultrastructure caused by greater zn stress were indicated by scattered nucleoli, condensed chromatin, almost empty nuclei with nuclear membrane disrupted and nucleoplasm flowing into cytoplasm, swollen and partly dissolved cristae of mitochondria, disrupted and collapsed chloroplast envelopes, and some dissolved thylakoids that flew into cytoplasm
超微結構的變化也呈現加重趨勢,低濃度處理的變化為細胞核變形、葉綠體膨脹、類囊體排列紊亂;嚴重的超微結構的損傷是核仁散開、染色質凝集,細胞核幾乎成為空核和核膜破裂,核質散出;線粒體脊突膨脹和部分溶解;葉綠體膜斷裂、消失和部分類囊體溶解和散到細胞質中。Prokaryotes lack organelles such as nucleoli, mitochondria, plastids, golgi apparatus, and do not exhibit cyclosis
原核生物無細胞器,如核仁、線粒體、質體、高爾基體等,不出現胞質環流。Cytosol the soluble fraction of cytoplasm remaining after all particles have been removed by centrifugation
胞質溶膠(細胞溶膠) :離心后細胞質可溶的部分,不包括細胞中的顆粒成分。The majority of mitochondrial proteins are encoded by nuclear genes, synthesized in the cytosol and subsequently imported into mitochondria
摘要線粒體的大多數蛋白質是由核基因編碼、細胞質合成,而最終運輸到線粒體。The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise
本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物( primer & primer ) ,以pbluescript ks +和puc18 / puc19質粒為載體,用常規的pcr方法從xanthomonascampestrispv . malvacearum ( smith ) dye等七株黃單胞菌( xanthomomasspp . )的基因組dna中克隆得到8個基因片段,分別命名為zhyf001 zhyf008 。The plasmid extraction from wild strains needs an appropriate method. first, lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1. but the expected result had not been got, and a lot of cracked plasmid dna fragments were got only
對於野生菌株的質粒提取,需要選擇合適的提取方法,我們首先採用了細菌細胞質粒提取常用的堿裂解法,但是未取得理想的效果,只得到了許多破碎的質粒dna片段。The main mechanisms of earthworms ' resistance to metal pollutants are also elaborated : ( 1 ) its lipid antioxidative enzyme system helps relieve the stress of oxidation ; ( 2 ) compartment and immobilization of metals ; ( 3 ) process of chelating and detoxicification ; ( 4 ) lysosome and cellular plasmid are activated to restrain activity of heavy metals
並闡述了蚯蚓對重金屬的主要耐性機制: ( 1 )脂質過氧化保護酶系統緩解氧化脅迫; ( 2 )分隔、固定作用; ( 3 )螯合解毒作用; ( 4 )溶酶體和細胞質粒抑制重金屬活性。These results are very important for us to further understand the genetic background, biological characteristics, evolutionary rule and the anti - schistosomajaponicum mechanism of microtus fortis at the molecular levels. the specific base changes of the dna fragme nt between the two subspecies are probably correlative with the animal immigration, survival conditions, and species evolution. the cdna library of microtus fortis bone marrow cells was transferred in situ to nylon membrane, which was divided into eight equals ( ga ~ - gh )
利用已經建立的東方田鼠骨髓細胞質粒cdna文庫,將cdna文庫轉化菌落印跡至尼龍膜,將膜均分成8份( ga gh ) ,制備基因池,分別培養、提取基因池質粒dna ,通過lipofect - 2000脂質體轉染技術,將基因池質粒dna導入hek293細胞, 48h后收集轉染細胞上清液,即條件培養基。分享友人