草達克 的英文怎麼說
中文拼音 [cǎodákè]
草達克
英文
tritac-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。But so far, there have not report about forest soil microbe and soil enzymatic activity in westen sichuan. the study ' s object is bitch forest. spruce forest, fir forest, chrysanthemum alp, willow community and two couch grass, so the research of the soil microorganisma, soil enzymatic activity in the subalpine coniferous forests in western sichuan is significant to china. the result shows that : 1. in the soil, the relativity of the soil microbial puantity is very prominence, the relation with the quantity of the soil microorganism is that : bacillus > actinomyceto > fungi ; the amount of the microorganism of physiological group sequence ranging from high to low is : aminate > bacteriumazotobacter > denitrify bacterium > nitrobacteria > cellulose decomposing bacteria. under the different vegetable community, the microbial quantity is that : s5 > s7 > s6 > s1 > s2 > s4 > s3
其中,各群落中土壤微生物總數以白樺純林( s5 )群落最多,每克干土中的含菌量達66 . 13 10 ~ 6個;其次是冷杉針葉林( s7 )群落,每克干土中的含菌量達43 . 41 10 ~ 6個:第三是雲杉針葉林( s6 )群落,每克干土中的含菌量達42 . 85 10 ~ 6個;第四是繡線菊-茅草群落( s1 ) ,每克干土中的含菌量達33 . 83 10 ~ 6個;第五是高山柳群落( s2 ) 、茅草( s4 )群落,每克干土中的含菌量分別為33 . 33 10 ~ 6個和33 . 08 10 ~ 6個;第六是茅草群落( s3 ) ,土壤微生物數量最少每克干土中的含菌量僅為23 . 12 10 ~ 6個。The dioxin levels in local pastures are also much higher than those found in imported grass varieties, such as alfalfa hay, bermuda grass and oat grass, which contain less than 0. 3 ng / kg of dioxin, according to the study
根據這份研究,本地牧草的戴奧辛含量也高出進口牧草許多,像是苜蓿、百慕達草、燕麥草等進口牧草的戴奧辛含量多在0 . 3奈克公斤以下。In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑轉基因bobwhite小麥中,利用pcr克隆的方法擴增出bar基因全長,並在原核表達系統中表達,鑒定表達蛋白的活性,將能夠正確編碼ppt乙酰轉移酶的bar基因片段,經過適當的修飾構建入真核表達載體。A known amount of lycopodium spores was added to each sample to calculate the concentration of pollen ( grains / gram ). pollen was examined at magnifications of 100 + and 400 + and identified using modern pollen collections and published atlases
P2 - 1 :該亞帶木本花粉的濃度達到了9 . 79粒克,而草本植物花粉的濃度相對于p1帶有所下降,氣候條件較好,植被為草甸草原。Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分為三個部分,其主要實驗方法及實驗結果如下:第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段( be644542 )入手,設計了基因特異性引物和載體特異性引物進行巢式pcr擴增,結合人類基因組草圖搜索法,從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna , genbank登錄號為ay040204 ,同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因, genbank登錄號為af395083 。A binary plant expression vector with osg6b " driving report gene gus was constructed and transferred into tobacco via agrobacterium tumefaciens mediation
將克隆的啟動子與報告基因gus相連,構建植物表達載體,通過農桿菌介導轉化煙草。Root distribution and canopy structure parameters of s. godejevii in different sandy land habitats ( semi - fixed sand dunes, fixed sand dunes and inter - dunes low land ), as well as species composition and their frequency in the herb layer of the scrubs, were compared the results were as follows : the population hi the semi - fixed sand dunes had the most developed root systems, followed by he population in the fixed sand dunes, and that hi the inter - dunes low land was the last
為了更有效地利用這一重要植物資源,本文對分佈於渾善達克沙地三種不同沙地生境(半固定沙丘、固定沙丘、丘間低地)黃柳灌叢的根系分佈,冠層特徵進行了研究,同時測定了灌叢下草本層植物種類組成及頻度,結果表明:半固定沙丘上的黃柳根系最發達(根系分佈深,數量大) ,固定沙丘的黃柳明顯不及前者,而丘間低地的黃柳根系最不發達。The expression plasmid called psugv - badfe was constructed by inserting ba - dfe gene into e. coli - b. subtilis shuttle vector psugv4 and the
將ba0fe基因克隆到大腸桿菌一枯草桿菌穿梭載體psugv4中,得到重組質粒psugv badfe ,然後轉化到枯草桿菌wb600中進行了分泌表達。F ) to retreat cultivation 1. 467 million ha for reforestation or recovering grass. g ) attheend, to adjust the proportion of cultivated land, forest, grassland from 7 : 6 : 1 to 1 : 1. 4 : 1. 2, this proportion is reasonable ratio for land use structure. for getting the above strategy targets, we must handle of the following relations : a ) the relation between development and environment protection ; b ) the relation between population and land resources ; c ) the relation between construction and farmland protection ; d ) the relation between quality and quantity of farmland ; e ) the relation between land using and economy development ; f ) the relation between land exploitation and landscape worth maintaining, etc. for getting the above strategy targets, we must also adopt some good measures : a ) to control the construction occupying farmland ; b ) to use land intensive ; c ) to improve low - yield farmland ; d ) to make regional land resources distribution effective ; e ) to make comprehensive profit ; f ) to make family planning continually ; g ) to promote urbanization, etc. by carrying out these measures, land resources in shanxi province can be used sustainable
同時,論文對土地資源利用潛力和未來趨勢進行了科學推理,提出了山西土地資源永續利用的戰略目標:到2015年,全省耕地面積穩定在433 . 3萬公頃左右,基本農田面積保持在366 . 7萬公頃左右,保證糧食產量100億千克;各項建設用地控制在14 . 2萬公頃,其中佔用耕地在7 . 9萬公頃,土地開發、復墾、整理補充耕地10萬公頃,改造中低產田10萬公頃,治理水土流失面積1 . 13萬公頃,發展園林草地146 . 667萬公頃(含退耕還林還草99 . 2萬公頃、園地6 . 7萬公頃) ;經過長期努力,使林草地總面積達到865 . 6萬公頃,耕地、林地、草地比例由現在7 6 1逐步調節到1 1 . 4 1 . 2較合理的比例。In addition, the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well. by using mrna differential display, 5 desiccation sensitive cdnas, 52 desiccation - induced cdnas, 21 up - regulated cdnas, 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained. the cloning, sequencing, homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as g protein, protein kinase, vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica
對其中5個脫水特異誘導表達牛耳草光合作廠j的脫水保護和復甦機理的cdna (包括可能與復甦能力有關的cdna )和3個磷酸鹽處理誘導表達的cdna進行克險測序、同源性探測和northern雜交檢測表明,牛耳草脫水過程中誘導表達的基因可能涉及到脫水脅迫的信號轉導「蛋白、蛋白激酶等) 、調節基因的級聯作用( vp3 , mad3樣基因等) 、結構基因產物調節細胞結構(包括細胞質膜)在脫水脅迫中的穩定性等。If one looks through mankind s earliest maps or to the genesis of greatest scientific achievements, we often discover a spectrum of fine engravings, sketches and drawings by the likes of einstein, watson and crick and da vinci
在觀察人類史上最早期的一些地圖或追朔最重要的科學成果的起源時,我們經常能發現一些由例如愛因斯坦華特遜克萊克或達芬奇等等偉大人物留下的雕刻草圖或圖畫。While nixon attended to white house business, chiao and i spent the first day of negotiation going through the existing draft line by line to confirm what was already agreed.
在尼克鬆處理白宮事務的時候,喬和我利用第一天的談判時間逐行審查現有的草案,肯定我們已經達成協議的部分。From korla tourists can reach the southern silk route by air or by bus through the taklimakan desert, second largest in the world. major scenic spots include the bayanbulak grassland, tiemen pass, bosten lake, ancient city of loulan, huangmiao temple of balguntay and the wonderful landforms of yadan
自此乘飛機或乘汽車穿越世界第二大沙漠-塔克拉瑪干沙漠就到達絲路南線,主要名勝古跡有巴音布魯克草原鐵門關,博斯騰湖樓蘭古城巴輪臺黃廟雅丹奇觀等。It absolutely feels substantially different. " having already announced that he intends to miss the clay - court season, agassi goes to next week ' s nasdaq 100 in miami knowing it will be his last event before the grass - court season begins in june
由於傷病等原因,阿加西宣布下周在美國邁阿密舉行的納斯達克100網球賽將是他今年參加的最後一項紅土場地的比賽,之後他要全力備戰6月開始的草地球場比賽。The results represented upon observation of stomatal movement showed that the density of stoma in the epidermal of transgenic tobaccos was much lower than that of wild type and the rate of opening and closing of stoma increased because of strong expression oi atdofl. 7
我們利用rt - pcr的方法,從擬南芥中克隆了與在氣孔中表達較多的stdof1基因的同源基因atdof1 . 7 ,構建了該基因的超表達載體,獲得了t1代轉基因煙草苗。The sacvgb gene was then cloned into e. coli - - b. sublilis shuttle vector psugv4 and a recombinant plasmid named psu - sacvgb was constructed. after transformation with b. sublilis wb600, the transformants successfully produced vhb intracellularly. the influence of vhb on b. subtilis is under investigation
將sacvgb基因克隆到大腸桿菌-枯草桿菌穿梭載體psugv4中,獲得重組表達質粒psu - sacvgb ,然後轉化枯草桿菌wb600 ,轉化子經2蔗糖誘導,該基因在枯草桿菌中表達。In this study, by combining rt - pcr and race, a full - length cdna encoding the lagurus lagurus zp3 ( lzp3 ) was isolated from the lagurus ' s ovary and cloned into a prokaryotic expression vector and a eukaryotic expression vector for further immunocontraceptive investigations
本論文研究工作採用rt - pcr與race相結合的方法,首次克隆了草原兔尾鼠zp3全長cdna並進行了序列分析,構建了真核表達質粒,為草原兔尾鼠免疫不育的研究奠定了基礎。Canada has a well - developed forage industry, making it an ideal destination for the chinese participants, says program coordinator and cigi director of feed dr. rex newkirk
培訓計劃協調人及加拿大國際穀物研究院飼料部門主管雷克斯紐科爾克rex newkirk博士說,加拿大擁有十分發達的牧草工業,因此它是中國學員的理想培訓地。Expression of ns6 gene of rice grassy stunt virus in e. coli and construction of its plant expression vector
水稻草矮病毒核衣殼蛋白基因克隆及在大腸桿菌中的表達分享友人