菌落雜交 的英文怎麼說

中文拼音 [jūnlàzájiāo]
菌落雜交 英文
colon hybridization
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 落 : 落名詞[方言] （北方對蓮花落的俗稱） a kind of folk song
  • 雜 : Ⅰ形容詞（多種多樣的; 混雜的） miscellaneous; varied; sundry; mixed Ⅱ動詞（混合在一起; 攙雜） mix; blend; mingle
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  1. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  2. Capacity evaluation of different media for isolating predominant phenol - degrading bacteria from activated sludge with community structure - specific dna probes

    微生物群落結構探針雜交評價不同培養基從活性污泥分離優勢菌群的能力
  3. In this paper, we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid

    以dig - oligo2為探針，菌落原位雜交篩選cosmid文庫，得到56個陽性重組子。
  4. Selected one of the 14 strains - s93, s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18, then transformed into dh5 a. filtering the clone with hybridization in situ, a 1 kb frament clone has been cloned

    使用sau3ai對基因組dna進行不完全酶切，回收2 3kb片段，與puc18質粒連接轉化大腸桿菌，利用地高辛標記探針，使用菌落原位雜交篩選轉化子；篩選到包含有約1kb外源片段的轉化子。
  5. In y2h, rapgap from c. elegans was used as bait to screen c. elegans cdna library. after - lth screen, 63 colonies were checked

    在酵母雙雜交系統中，將ppc97 rpg 」與ppc86七dna文庫共轉化入酵母菌y190細胞，經過一lth篩選后，有63個菌落擬為陽性菌落。
  6. The distribution and amount analysis of these bacteria in different layers of core sediment indicated that there was an intact cycle that coupled sulfur metabolism with methane metabolism existed in this area, which may be the microbial response to the environment because there was seldom similar bacteria detected from " manganese nodule " area sediment by dna - dna hybridization with specific oligonucleotide probe and 16s rdna clone library analysis

    而16srdna克隆文庫分析和dna - dna雜交的結果表明「結核」區沉積物中這兩類細菌數目很少，說明「暖池」區沉積物中的微生物群落結構特徵是對環境因素的一種響應，同時也可能是影響該海區深海及海洋環境的一個重要因素。
  7. Through in situ hybridization and colony pcr, a positive recombinant plasmid was isolated from the genomic library and sequenced. the inserted dna fragment was about 4. 3 kb

    將pcr擴增片段用地高辛標記探針，利用原位雜交和菌落pcr從基因文庫中獲得含有陽性信號的重組質粒。
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