蛋白分級分離 的英文怎麼說

中文拼音 [dànbáifēnjífēnlí]
蛋白分級分離 英文
protein fractionation
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 級 : Ⅰ名詞1 （等級） level; rank; grade 2 （年級） any of the yearly divisions of a school course; gra...
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。
  2. Abstract : the effects of terbium ion on the conformation of calmodulin and on the interaction between calmodulin and melittin have been studied by the endogenous fluorescent spectrometry of calmodulin and melittin, and the sensitized fluorescent spectrometry of terbium ion, respectively. the results show that terbium ions have a tight binding site in the i and ii metal - binding sites of calmodulin. the conformation of calmodulin induced by terbium ion can bind melittin and transfer the tryptophane residue of melittin to a relatively hydrophobic environment, while the binding of melittin to calmodulin produces effect on the binding orders of terbium ion in camodulin. results from ft - ir spectrometry have revealed that upon binding of lanthanum ion, apo - calmodulin undergoes a conformational change with the increase of - helix content and the decrease of - sheet content. melittin ' s binding to calmodulin has no effect on its conformation induced by the binding of lanthanum ion to calmodulin

    文摘:分別用鈣調蛋白和蜂毒素的內源熒光光譜以及鋱離子的敏化熒光光譜考察了鋱離子對鈣調蛋白構象變化以及對鈣調蛋白與蜂毒素相互作用的影響.結果表明，鋱離子首先結合在鈣調蛋白的第和第位點，鋱離子不影響鈣調蛋白與蜂毒素的相互作用，蜂毒素與鈣調蛋白作用后不影響鋱離子在鈣調蛋白上的鍵合順序.傅里葉變換紅外光譜結果表明三價的鑭離子與鈣調蛋白作用使鈣調蛋白的螺旋結構增加，折疊結構減少，與鈣離子對它的二級結構影響相類似.稀土離子在鈣調蛋白-蜂毒素復合體系中主要與鈣調蛋白作用
  3. A water soluble crude polysaccharide in the cells has been isolated from the zymogen thread of hericium erinaceus pers. after the crude polysaccharide has been deamylumized by the combined methods of enzyme and sevage, the four graded component - hpa, hpb, hpc, hpd - would be abstracted by the method of ethanol grading

    從猴頭菌（ hericiumerinaceuspers ）發酵菌絲體中提取出胞內水溶性粗多糖，經酶法和sevage法聯合脫蛋白后，用乙醇分級法分離出hpa 、 hpb 、 hpc 、 hpd四種級分。
  4. - acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography

    本文對來源於枯草芽孢桿菌（ bacillussubtilis ） 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟，得到sds - paeg電泳純，通過n末端氨基酸序列分析驗證酶蛋白的純度。
  5. Part i this paper has minutely studied the interaction between ag ( i ) and serum albumin. the binding of ag ( i ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 5. 4 ). the scatchard analysis indicates that there exists several strong binding sites of ag ( i ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of ag ( i ) with hsa or bsa using uv - visible spectrometry at ph ( 5. 4 ), which shows that the binding between ag ( i ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for ag ( i ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has been also studied by resonance light - scattering spectrum ( rls ) and flurescence quenching

    第一部分：等離子點ph （ 5 . 4 ）條件下，用平衡透析法和紫外光譜，熒光光譜，共振散射光譜研究了ag （ ）與人血清白蛋白（ humanserumalbumin ，簡稱hsa ）或牛血清白蛋白（ bovineserumalbumin ，簡稱bsa ）的結合。 scatchard圖分析表明， ag （ ）在hsa或bsa中有強弱兩類結合部位，通過計算機擬合獲得結合的逐級穩定常數值。紫外掃描發現ag （ ）與hsa或bsa的結合存在滯後效應，表明ag （ ）與hsa或bsa的結合可能誘導蛋白質構象發生緩慢變化（ a - b ） ，測得並討論了這一構象變化的速度常數和活化參數。
  6. Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively

    Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。
  7. The plant hormone ethylene has a profound role in plant growth and development. a number of ethylene signaling elements have been isolated and identified. although nuclear cascades in ethylene signal transduction pathway from ein3 to erf1 to pr proteins has been established in arabidopsis thaliana, a similar route from ein3 to erebps to fruit ripening and senescence has not been elucidated in lycopersicon esculentum ( tomato )

    乙烯對植物的生長發育起重要的調控作用，隨著乙烯信號轉導研究的不斷深入，許多乙烯信號轉導組分已被分離和鑒定，並在模式植物擬南芥中已建立乙烯信號傳遞過程中的核內級聯反應： ein3 erf1 （ erebps ）病原相關蛋白，但在番茄中還沒有建立ein3 erebps果實成熟相關基因的相似路徑。
  8. We have demonstrated isolation of single - class plasma lipoproteins based on the immunological method associated with streptavidin ? biotin capture technology, and the lipoprotein cholesterol was consecutively quantified on the same strip without handling of reagents ( 11 )

    我們還論證了基於免疫學方法結合鏈親和素捕獲技術分離單一級別血漿脂蛋白，並且在同一條帶上不用對反應物進行操作就能連續定量檢測脂蛋白膽固醇。
  9. It is a light yellow flour centrifugal separated, grinded, disinfected, screened which is made from low - temperature de - solvented white soy meal and it is a high - protein, high - solubility, low - fat and good - quality feedstuff which can be made into feed for domestic animals, birds ， fishes and the special farming

    採用優質大豆生產的低溫脫溶白豆粕為原料，經離心分離、研磨、滅菌、篩分等工藝精加工而成的淺黃色粉狀飼料級脫脂大豆蛋白粉，具有高蛋白、高溶解性、低脂肪等特性，是畜、禽、魚及特種養殖飼料的優質原料。
  10. In addition, we are highly interested to study non - covalent interaction involved in the protein quaternary structure by using various biophysical methods ( including analytical ultracentrifugation and nanospray - esi - ms ) as these approaches can be applied to decipher protein - protein interaction

    此外，我們也使用一些生物物理的方法，例如分析型超高速離心、電子噴?離子化質譜技術，探討在蛋白質四級結構中的非共價性結合的交互作用，希望所得的結果能夠應用在蛋白質與蛋白質的交互作用。
  11. After ultrafiltration and rough separation with ammonium sulfate and anion - exchange chromatography, the recombination protein of ctla4 extracelluar domain was rectified from the ferment supernatants

    表達菌甲醇誘導發酵；發酵上清液經超濾、硫酸胺粗分級分離以及陰離于交換層析，純化出ctla4胞外區蛋白。
  12. The protein has the immunogenicity of human calcitonin verified by western blot analysis. after optimization of condition, fusion protein was concentrated by ultrafiltration and cleaved by factor x a, then recombinant novel hct was obtained

    表達條件優化后，可溶性蛋白的表達量占胞內總蛋白的10 。經超濾濃縮后，融合蛋白在xa因子作用下產生重組的新型降鈣素，進一步分離純化得到該蛋白的初級純品。
  13. A large number of these proteins have been isolated and their primary and secondary structures have been identified

    已經分離出許多物種的非特異性脂轉移蛋白，其一級和高級結構均已闡明。
  14. With high quality chinese medicine sauce, ckfood ' s veggie mutton stew has its unique mutton taste and great sauce flavor. this is the product you can ' t miss it

    簡介新鮮香菇頭經按摩分離抽絲后，加入天然組織型大豆蛋白漿與精燉后的高級中藥湯汁融合其中，更加提升小羊排獨特的口感與嚼勁。這就是我們要的天然素材。
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