蛋細胞質 的英文怎麼說
中文拼音 [dànxìbāozhí]
蛋細胞質
英文
ooplasm- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 質 : Ⅰ名詞1 (性質; 本質) nature; character; essence 2 (質量) quality 3 (物質) matter; substance;...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
-
Localization of specific glycoproteins of sperm cell membrane from
玉米精細胞質膜特異糖蛋白膠體金免疫定位As for the sub - cellular locations of cortactin, laser confocal scanning microscope pictures shew that in cleavage stage, cortactin was distributed ubiquitously in the cytoplasm with the exception that in cells undertaking cytokinesis, positive stained signals were detected in the cleavage furrow
在利用激光掃描共聚焦顯微鏡進行的皮層蛋白亞細胞定位的觀察中發現,卵裂期,皮層蛋白在整個細胞質中分佈,在正在進行胞質分裂的卵裂溝中染色較強。Enucleate cytoplasm can still synthesize protein.
去核的細胞質仍然能合成蛋白質。The majority of mitochondrial proteins are encoded by nuclear genes, synthesized in the cytosol and subsequently imported into mitochondria
摘要線粒體的大多數蛋白質是由核基因編碼、細胞質合成,而最終運輸到線粒體。In animal and lower plant cells, cytoplasmic dynein involved in not only trafficking of subcellular organelles and target molecules, but also organizing and positioning of organelles such as golgi apparatus
細胞質力蛋白在動物和低等植物細胞中不僅參與細胞內細胞器和靶分子等的運輸,還參與高爾基體等細胞器的組裝和定位。Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法,用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定,結果表明:抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應,同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在,表明在植物中有類三磷酸肌醇受體蛋白的存在;用免疫膠體金方法,發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。Any of the proteinaceous cylindrical hollow structures that are distributed throughout the cytoplasm of eukaryotic cells, providing structural support and assisting in cellular locomotion and transport
蛋白質圓柱形空心結構,廣泛存在於真核細胞的細胞質中,為細胞內的移位和傳輸提供結構支持和協助。By sds - page and immuno - blotting, we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen. under confocal laser scanning microscopy after immunoflurescence labeling, we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube
免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現,類細胞質力蛋白中間鏈在百合花粉管中存在於顆粒狀細胞器上;免疫熒光雙標及激光共聚焦掃描顯微鏡觀察發現,百合花粉管中類細胞質力蛋白中間鏈和微管存在部分共分佈。Salt treatment had effects on growth, succulence and some physiological parameters. in present study, suaeda salsa seedlings were treated with different salts and isoosmotic peg to examine the succulence and some physiological parameters. the hydraulic conductance ( lo ) of the roots, the water permeability of protoplasts and western blot analysis of aquaporins in plasma membrane and tonoplast under nacl were determined
本實驗以鹽生植物堿蓬幼苗為材料,用不同的鹽及與nacl等滲的peg處理,測定肉質化及有關生理指標,並測定nacl處理下植物根的導水性,原生質體的水滲透性,並在分子水平上進行了細胞質膜及液泡膜水孔蛋白免疫雜交分析。The results indicates that the expression of ap - 2 a is concentrated in nuclear extract, whereas the major expression of testin is in cytoplasmic extract, and the expression of actn1 is in both nuclear and cytoplasmic extract. the interaction between ap - 2a and testin or actnl should be comfirmed by further test
結果顯示三種蛋白在hela細胞中均有表達,且ap - 2主要表達于細胞核提取物中; testin大部分表達于細胞質提取物中,細胞核提取物中也有少量; actn1既表達于細胞質又表達于細胞核提取物中。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。Recently, a great number of biochemical analyses and immunoctochemical experiments have proved that actin presents in nuclei and chromosomes in plants and animals, showing that actin is involved in the nuclear processes such as chromosome condensation, rna transcription and so on
肌動蛋白主要存在於細胞質中,是細胞骨架微絲的重要組成成分,受多種肌動蛋白結合蛋白的調控,參與與細胞形態和結構相關的多種生理過程。Recent studies have indicated the existence of dynein heavy chain and light chain in higher plants, but no data about dynein intermediate chain in higher plants have been reported
近來的研究表明在高等植物細胞中存在細胞質力蛋白的重鏈和輕鏈,但力蛋白中間鏈在高等植物細胞中還未見報道。These results suggest that the cytoplasmic dynein intermediate chain - like protein exists in lily pollen, and it distributes mainly on either cis - and trans - golgi - associated vesciles
以上的結果表明類細胞質力蛋白中間鏈存在於百合花粉及花粉管中,並且在百合花粉管中順面和反面高爾基體附近的囊泡膜上均有分佈。After treatment with bfa, immunoflurescence labeling and confocal laser scanning microscopy indicated that the cytoplasmic dynein intermediate chain - like protein in lily pollen tube was insensitive to bfa treating, but spectrin - like protein was sensitive to it under the same condition
用bfa處理百合花粉管后,通過免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現類細胞質力蛋白中間鏈對bfa不敏感;同樣的處理發現類紅膜肽對bfa敏感。By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現,抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應;進而通過免疫金標結合電子顯微鏡觀察發現,大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上;統計結果表明,類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。The new synthesized protein was led to endoplastic reticulum cavity by eukaryotic secretory signal peptide sequence and then anchored to innerwall of endoplastic reticulum by kdel sequence, which interdicted the process of protein entering golgi body and cytoplasm, and then avoided heterogeneous glycosylation modification of foreign protein and prolonged the disappearance of half life of protein in organism. 2
真核分泌信號肽序列可以引導新合成的蛋白質進入內質網腔, kdel序列將進入內質網腔的蛋白質錨定在內質網內壁上,從而阻斷了蛋白質進入高爾基體和細胞質的過程,進而避免了外源蛋白質的異源糖基化修飾,延長了蛋白質在生物體內的半衰期。An unusual rice calmodulin isoform, oscam61, was first obtained in our lab, which contains an n - terminal cam domain and a c - terminal basic extension with a potential prenylation site. in vitro activity assays confirm oscam61 as a functional calmodulin. using the green fluorescent protein ( gfp ) as a visual marker, we further studied subcellular localization of oscam61 in stably transformed tobacco cells
利用綠色熒光蛋白( greenfluorescentprotein , gfp )作為標記,研究了oscam61在煙草細胞中的定位, gfp - oscam61融合蛋白(具有開放的異戊烯化修飾位點)定位於細胞質膜和細胞器膜上,而oscam61 - gfp (異戊烯化修飾位點被gfp封閉)定位於細胞核的核質中。The protein encoded by orf1 show strong homology with cation efflux family protein from geobacter sulfurreducens pca [ identities = 65 / 272 ( 23 % ) ], and contains mmt1 - like putative conserved domains kog1485, mmt1 contains conserved domains kog1485 is mitochondrial fe2 + transporter ( cation diffusion facilitator superfamily ). so it can be considered that the protein encoded by o rf1 is fe2 + transporter involved in magnetosome biosynthesis in magnetospirillum gryphiswaldense msr - 1
經同源比較和保守性結構域分析,推測orf1編碼的蛋白具有fe ~ ( 2 + )轉運功能,在磁小體合成過程中將細胞質中fe ~ ( 2 + )轉運到磁小體囊泡中,合成磁小體的同時去除fe ~ ( 2 + )對細胞的毒害作用。Our previous work has also shown that there is a cam - binding site within maize cytoplasmic hsp70 and that hsp70 binds cam in a ca2 + - dependent manner ( sun et al, 2000 ). the high conservation of cam - binding suquence implies that the binding of cam to hsp70 might have an important biological function
先前我們在玉米中,通過體外結合和競爭抑制實驗證明了熱激蛋白家族中最重要的成員之一純化的細胞質hsp70能與cam結合,並且證明了玉米細胞質hsp70中存在一個非常保守的21個氨基酸組成的cam結合序列。分享友人