轉化基因 的英文怎麼說

中文拼音 [zhuǎnhuàjīyīn]
轉化基因 英文
transformed gene
  • 轉 : 轉構詞成分。
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 轉化 : 1. （轉變） change; transform 2. [化學] inversion; conversion
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  3. Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways

    我們發現，低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應，並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降， dna聚合酶譜發生改變，應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活，細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇，細胞信號轉導通路camp - pka - creb和jnk sapk被激活。
  4. Screening target ' gene of artemisinin antimalarials using drug - western from cdna expressing library : 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether was linked to bsa by using of edc cross ! inker and the product acted as drug - probe

    對重組pmd一18一t克隆載體及pqe一30表達載體雙酶切，提取tctp基因和pqe一30空載體並使二者重組，然後轉化m15 ，挑取陽
  5. Advances on genetic transformation of cotton shoot apical meristem via gene - gun bombardment

    以棉花莖尖分生組織為受體進行基因槍轟擊轉化的研究
  6. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  7. A genetic transformation model for the brown seaweed undaria pinnatifida has been primarily set up by using micro - particle bombardment as the method, female or male gametophytes as the gene recipients, hybridization as the regeneration route and chloramphenicol, hygromycin or basta as selective reagents

    本文從轉化受體、轉化方法、報告基因、再生途徑、篩選方法等方面對裙帶菜的遺傳轉化進行了研究。首先，分離並建立了裙帶菜雌雄配子體的無性繁殖系，進行了裙帶菜的不同再生途徑的研究。
  8. Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )

    獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片（基因槍93片，農桿菌88片） 。
  9. E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd

    以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ，以輔助噬菌體m13k07超感染，進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。
  10. Transformed shoots were selected on solidified medium containing kanamycin. ten kanamycin resistant transformants were obtained by direct or induction calla. these transformants were checked by pcr, pcr - southern blot and southern blot, confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad

    對所得到的抗性轉化株進行了pcr 、 pcr - southernblot 、 southernblot檢測，其中2株呈陽性，證明vp4基因已整合到苎麻基因組dna中。
  11. The results also revealed that it was a reasonable choice to use carbenicillin as the antibiotics of inhibiting growth of remnant agrobacterium after co - culture, and when hygromycin was used as the selective agent, continuous selection at low concentrations ( 50 ~ 150mg / l ) produced the highest numbers of transgenic plants without escapes

    試驗表明，在農桿菌介導高羊茅遺傳轉化中，抑菌劑宜選用梭節青霉素;以潮黴素作為選擇劑時，採用低濃度（ 50一15om留l ）連續篩選的方式比較合適，在該方式下，獲得的轉基因植株較多。
  12. Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded

    經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。
  13. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  14. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  15. On the basis of all the conclusions above, the thesis preparatorily advances that there is another factor of link transaction behavior affecting vertical relationship, and gives primary definition of relative concepts. the thesis advances that link transaction behavior can be classified into priority transaction, information informing, risk co - affording. in order to form effective vertical transaction relationship, the transaction parties will positively process link transation behavior, including transforming incontractible variants into contractible variants or analogously contractible variants

    在此基礎上，本論文初步提出影響縱向關系的因素應該包括關系交易行為這一重要變量，並對關系交易行為和關系交易行為的相關概念作出初步的定義，初步提出企業的關系交易行為可以分為交易優先、信息告知、風險共擔三類，並在此基礎上初步分析其中的機理，並根據分析結果提出，當事人交易的過程是不斷提升關系的強度的過程，為了形成有效的縱向交易行為，當事人會主動進行信息告知等關系交易行為，通過把不可寫入合同的變量轉化為可以寫入合同或者可證實的變量來獲得有效交易。
  16. Expression vectorion construction of hepatitis b surface antigen gene and transformation to crowtoe lotus corniculatus

    乙肝表面抗原基因表達載體的構建及對百脈根的轉化
  17. Plants are thought to remove na + from the cytoplasm by transporting it into the vacuolar or out of the cell using na + / h + exchangers localized in the vacuolar and plasma membranes, respectively. sos1 encoding a plasma membrane na + / h + antiporter and atnhxl encoding a vacuolar na + / h + antiporter were isolated from glycophytic arabidopsis thaliana, and overexpression of atnhxl and sos1 in arabidopsis thaliana increased the salt tolerance of transgenic plants significantly

    目前，擬南芥細胞內控制na ~ +外排的基因sos1及離子區隔化基因atnhx1均已克隆， sos1及atnhx1在擬南芥中的過量表達顯著提高了轉基因植株的耐鹽性，開創了降低na ~ +毒害的基因操作新途徑。
  18. Bar gene also is one of widely used selective marker gene. the cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology

    而且bar基因也是迄今為止應用最為廣泛的一個抗除草劑選擇標記基因，克隆出bar基因對植物的遺傳轉化，基因表達和生理學研究都有重要的作用。
  19. The constuction of plant expression vector, tissue culture of cabbage and gene transformation conditions were studied to obtain ubic transformants. ubic gene was obtained from the escherichia coli. by pcr amplication and was ligated to puc118 vector

    為獲得轉ubic基因甘藍，對植物基因工程載體的構建、甘藍的組織培養以及基因轉化條件進行了研究。通過pcr方法從大腸桿菌基因組中擴增得到了ubic基因，擴增產物克隆到puc118載體，轉化大腸桿菌jm109 。
  20. To make the ti plasmid pbi121 - nhap for transformants the tobacco ( nc89 ), at the same time to make the pemu - mcs - n - nhap and pwubi. tml - nhap for transformants the yan wheat 18. we getted 4 transgenic tobaccos, measured the ability of salt tolerance by treatment in 150 mmol / 1 nacl, found that the transgenic tobaccoes can growth and the control tobaccos ca n ' t

    通過比較轉synnhap基因煙草與對照煙草的耐鹽性，發現4株轉synnhap基因的煙草再生苗在含150mmnacl的培養液中處理兩周后，植株外觀無明顯變化，葉片生長情況正常，而對照植株的生長則受到抑制，葉片明顯萎蔫、失綠並最終脫落。
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