轉基因動植物 的英文怎麼說
中文拼音 [zhuǎnjīyīndòngzhíwù]
轉基因動植物
英文
genetically modified animals and plants-
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。To verify the integration of st901 gene into transgenic plants genome, the stable transgenic plants were analyzed by pcr and southern blotting. the aberrant phenotypes were observed in pollens and anthers of the transgenic plants. most of pollen grains in the transgenic plant were distorted, shrunken, invagination and not stained with the solution of acetocarmine
通過對轉基因植株花粉、花藥形態的觀察和花粉活力的測定，表明st901啟動子驅動的st901基因在轉基因植株中的表達造成花粉嚴重敗育，花粉粒皺縮，扁癟、塌陷，缺乏內容物；轉反向表達載體的馬鈴薯花粉育性僅為對照的5 . 2 ，育性下降94 . 8 。7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp
7 、首次以fam熒光素標記探針5 』端作為發光基團，以tarma標記探針3 』端為淬滅基團，以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a ）為檢狽目標，設計、篩選出特異性引物和探針，優化實驗參數，建立了轉基因植物通用性熒光pcr定性檢測方法體系。The transgenic plants have been studying for more than ten years, which have some advantages including that the plants are autotrophy not requiring expensive media or strict culture conditions, and the plant viruses cannot infect human, compared with other systems such as microbe fermentation and transgenic animals
與微生物發酵、動物細胞和轉基因動物等生產系統相比，轉基因植物不需要昂貴的設備和嚴格的培養條件，具有光合自養、成本較低，植物病毒不感染人類等優點，已成為一類比較廉價和安全的生物反應器。This gene, artificially put under the control of camv 35s, was introduced into tobacco with the aid of agrobacterium, and the transgenic plants obtained were identified by gus staining, pcr and southern blot analysis
將arge與組成型啟動子camv35s相連，構建植物表達載體，通過農桿菌介導轉化煙草。經過gus染色、 pcr及southern鑒定獲得了轉基因植株。To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。Gene engineering is the one of science and technologies which are developing fastly. with the developing of gene - group school and element engineering school, specially " the human beings gene - group plan " having been completed, gene engineering has already developed a new history stage. trans - gene plants and animals, trans - gene foodstuff, gene diagnosis. gene therapy. gene medicine, ects, all these spur the people ' s nerve, and gene engineering becomes the focus. people expect gene engineering to give them the property, health and happiness
基因工程是當前科技發展最為迅速的領域之一，隨著基因組學和分子工程學的發展，特別是「人類基因組計劃」的初步完成，標志著基因工程發展到一個新的歷史階段。轉基因動植物、轉基因食品、基因診斷、基因治療、基因藥物等基因產業的異軍突起，刺激著普通民眾的神經，成為人們關注的熱點。Acbf is a transcription factor that combined with the ac - rich region of the promoter region. it has been cloned at arabidopsis thaliana, nicotiana tabacum and so on
Acbf編碼了結合在苯丙氨酸解氨酶啟動子ac富集區上的一個轉錄因子， acbf基因已經在擬南芥、煙草等植物中得到了克隆。A binary plant expression vector with osg6b " driving report gene gus was constructed and transferred into tobacco via agrobacterium tumefaciens mediation
將克隆的啟動子與報告基因gus相連，構建植物表達載體，通過農桿菌介導轉化煙草。Pcr analysis indicated that all lines had been integrated of ssmapkk. northern analysis revealed the presence of expression of ssmapkk mrna in transgenic lines. in principle, ssvp overexpression can increase proton electrochemical gradients across the vacuolar membranes, which permit the secondary active transport of na + and solute molecules
理論上， ssop的過量表達可增加轉基因植株細胞跨液泡膜的質子電化學梯度，為次級轉運提供驅動力，從而增加可溶性物質和na十向液泡內的轉運，提高轉基因植株的抗旱和抗鹽性。By means of plant genetic engineering, foreign insects resistance gene can be transferred into plant cell. we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method. and obtained the transgenic mustard plants. the main results are as follows : 1. isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves. the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers
本實驗是利用植物基因工程獲得抗蟲的轉基因芥菜植株，結果如下： 1豇豆胰蛋白酶抑制劑基因的分離分別提取豇豆種子、子葉及葉片的總rna ，逆轉錄成cdna 。以豇豆胰蛋白酶抑制劑基因兩端的序列為引物，用rt - pcr的熱啟動方法從上述cdna中擴增出目的基因片斷。The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin
本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。5. in this study, we have cloned camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryia ( b ) genes used as positive collate
5 、克隆了camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1ao ）的陽性質粒作為轉基因植物檢測的陽性對照。These improvements bring many benefits to farmers, such as reducing the amount of hard physical labour required to grow the crop and simplifying weed control
這些轉基因操作為種植者帶來了許多好處，如減少了種植這些作物所需的重體力勞動勞動者數量，並使控制雜草變得簡單。As many other countries, chinese consumers also demonstrate great variance in their acceptance and purchase will towards gm foods under different food categories. to be more specific, the consumers are most willing to accept and buy gm foods that improve nutrition, followed by pest - resistant types foods with those for extending shelf life being the least preferred
盡管如此，我國消費者對轉基因食品的接受程度和購買意願在不同的食品之間還是表現出了較大的差異，具體表現為：改善營養的最高，抗病蟲害的次之，延長貯存期的最低；轉基因植物性食品高於動物性食品。And the fusion protein could been purified quickly by thiobond ? resin. this was key step to produce cmiv largely by fermentation. meanwhile, the structure of the mutation ii of cmiv showed a new way to design peptide of cecropin which have the targeting sequence to tumor cell
以上研究的意義： 1 、初步解決了抗菌肽cmiv突變體在大腸桿菌中高效可溶性表達的問題，為進一步大量生產cmiv基因突變體打下基礎； 2 、為進一步設計具有特異導向性的抗菌抗腫瘤cmiv突變體基因和表達以及以後進行動植物轉化打下基礎。In abroad, the study of integration site used for transgenic detection had just begun. in this study, according to the collection of the global commercialized transgenic crops, select seven exogenous genes which basically cover the total commercialized crops, namely camv35s and fmv promoter, nos terminater, mark gene nptii, and aim genes pat, epsps and cryia ( b ). use endogenous 18srrna gene as collate, design a large pairs of specific primers, screen the optimum primers groups, optimized the test condition and parameters, establishing the qualitative pcr detection system
本研究根據收集的國內外已商品化的轉基因作物品種，選擇了能基本覆蓋商品化轉基因品種的7個外源基因，即： camv35s 、 fmv啟動子、 nos終止子、 npt標記基因和目的基因pat 、 epsps 、 cryia （ b ）作為篩選目標，以植物18srrna基因作為內源參照基因，設計了多對特異性引物，並篩選出最佳組合，優化了檢測條件和參數，建立了pcr定性檢測方法體系。With all these results, we may make such a conclusion that kst1 promoter is quite useful to realize the spatial and quantitative expression patterns of atnced3 in order to improve water use efficiency in plants by manual manipulation
綜合以上實驗結果，可以得出初步結論：利用atnced _ 3基因來人工調控植物的水分利用效率， kst1啟動子是比較理想的實現植物轉基因表達調控的基因開關系統。Leaf disks from tobacco are infected with cultures of a. tumefaciens transconjugants harboring the different plasmids, and transgenic plants are generated by the leaf disk procedure, plant extracts are analyzed by the fluorescent method for detecting gus activity. conclusions are drawn from our experiments and shown below : 1
融合啟動子與gus報告基因融合構建相應的植物表達載體，並以含camv35s啟動子的表達載體pbi121作對照轉化煙草nc89 ，利用熒光分光光度計法定量測定各轉基因煙草植株的gus基因表達活性。To explore the feasibility of ib edible vaccine, we have transformed si gene of ibv into potato and researched the immunogenicity of it ' s expression product. the findings are as follows : 1. a pair of primers for ibv si gene were designed and synthesized according to the published nucleotide sequence of ibv si gene and multiclone sites of expression vector pbi121
為了探索ibv可食性疫苗的可行性，我們進行了轉基因馬鈴薯表達ibv免疫原基因及其表達產物免疫原性的研究，取得以下結果： 1根據周繼勇等報道的ibv - zj971毒株s1基因核苷酸序列和pbi121植物表達載體的多克隆位點，設計併合成引物，以含s1pbs質粒為模板擴增s1基因，將擴增片段定向克隆到pbi121質粒的35s啟動子下游。分享友人