轉基因大鼠 的英文怎麼說
中文拼音 [zhuǎnjīyīndàshǔ]
轉基因大鼠
英文
transgenic rat-
Effect of angiotensin ii on vasopressin gene transcription in the hypothalamus of rats
血管緊張素ii對大鼠下丘腦內血管升壓素基因轉錄的影響2. rat orthotopic liver trarlsplantation was performed in the strong rejecter combination of bn ( rtl " ) to lew ( rtl l ). this al1ogenic rejection model was stable and the survival time without immunosuppression agents was less than three weeks ( the average survival time was l 6. 6 i 2. 5 days, n = 5 )
建立了大鼠bn ( rt1 ~ n ) lew ( rt1 ~ l )原位肝移植同種異體排斥模型,該模博士學位論義基因轉移hu la個ig誘導大鼠移植肝免疫耐受的實驗研究中文摘要型排斥反應時間較為恆定,在不應用免疫抑制劑的情況下,生存期3周(平均生存時問人66士2Effect of acupuncture on the expression of choline acetyltransferase mrna in the brain of ovarietomized rats
針刺對去卵巢大鼠腦內膽堿乙酰轉移酶基因表達的影響Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen, fibronctin, laminin ln, and the ecm metabolism influencing factors, including mmp2, tissue inhibitor of metalloproteinase timp2, transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system
動態檢測各組大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ,末次給藥后測定大鼠血漿糖化血紅蛋白hba1c透射電鏡觀察各組大鼠腎臟超微結構改變,應用免疫組化技術及圖像採集分析系統測定各組大鼠腎臟組織中型膠原c纖維連接蛋白fn層粘連蛋白ln的表達,測定影響ecm代謝的基質金屬蛋白酶2 mmp2基質金屬蛋白酶抑制劑2 timp2及轉化生長因子1 tgf 1的表達。In vitro cytotoxicities of gcv and ctl to rat mammary cancer cells transfected with hsv - tk b7 gene
7基因轉染的大鼠乳腺癌細胞殺傷的體外實驗Objective : the purpose of this study was to screen the signal transduction genes in rat liver - cancer tissues down - regulated by invigorating spleen and regulating qi
摘要目的:篩選出健脾理氣法明顯下調大鼠肝癌組織中與信號轉導有關的基因。Design : induced by administration of den solution, we got the rat liver cancers model to observe the regulation effects of invigorating spleen and regulating qi to genetic transcription, which is relevant to the transcription and signal transduction in genome of rats - liver - cancer tissues, controlled treatments about chemotherapy of medicine - ftorafurum, and the two tcm treatments including expelling toxin by cooling and promoting blood circulation to remove blood stasis
方法:以den誘導大鼠肝癌形成為模型,以西藥喃氟啶化療、中醫清熱解毒、活血化瘀治法為對照治療,採用基因晶元技術,觀察健脾理氣法對大鼠肝癌組織基因組轉錄水平及信號轉導相關基因轉錄的調控作用。Conclusion : the genetic chips revealed that the method of down - regulated the genes associated with its signal transduction of liver cancer ' s tissues, referring to plenty of links in the lattice of transduction, among the functional molecule targets of these different treatment of tcm to prevent and cure liver cancer
討論:基因晶元很好地揭示了不同中醫治法各自防治肝癌的分子作用靶點,其中,健脾理氣法下調大鼠肝癌組織與信號轉導有關的基因轉錄中,涉及到細胞信號轉導網路的多條通路的多個環節。In the first part of the present work, the expression changes of angiotensinogen ( agt ) and angiotensin - converting enzyme ( ace ) as well as its time course characteristics were investigated by rt - pcr, in situ hybridization and western blotting. we also prepared the polyclonal antiserum against agt for the following work
故本工作應用rt - pcr和原位雜交、 western印跡分析等方法對模擬失重大鼠不同部位動脈血管血管緊張素原( angiotensinogen , agt )及血管緊張素轉化酶( angiotensin - convertingenzyme , ace )基因表達變化的時程特徵進行了觀察,並為后續工作制備了抗agt多克隆抗血清。Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence
以pcr擴增的人血清白蛋白( hsa )基因片段為探針,從人的基因組文庫中雜交篩選的陽性克隆中,經測序分析,有一個克隆含有全長hsadna ,用從其它的陽性克隆中選取兩種dna片段,即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ,與人hsadna一起,進行顯微共注射,成功制備了轉多基因小鼠。The experiment purpose is to confirm that there exist il - 2rand er in c6 glioma cell line whether at protein level or mrna level, and explore the effects of il - 2 and estradiol on the cells as well as the possible mechnism of signal transduction of them
本次研究擬從基因與蛋白水平系統的證實c6大鼠神經膠質細胞有il zr與er表達,並分別初步探討ilz與雌激素對該細胞基本生理活動的影響,及其可能的信號轉導途徑。Expression of target gene in mesenchymal stem cells after transfection of basic fibroblast growth factor gene
堿性成纖維細胞生長因子基因轉染大鼠骨髓間充質幹細胞后目的基因的表達Angiogenic effect of bone marrow mesenchymal stem cells transfected with human vegf gene on myocardial infarcts in rats
基因轉染骨髓間充質幹細胞對大鼠心梗區血管新生的影響5 dvork hf, brown lf, detmar m. et al. vascular permeability factorp vascular endothelium growth factor, microvascular hyperpermeability, and angiogenesis. a j pathol, 1995, 146 : 102921039
2姜寧,高煒,王日勝,等.肌肉電轉促血管生成素21基因治療大鼠下肢血管閉塞病.中國介入心臟病學雜志, 2001 , 9 : 1012103Purification of the recombinant expressed endostatin in this work we have successfully cloned mouse endostatin gene, and constructed pbv220 - endostatin expression plasmid that is a non - fusion expression vector. the positive pbv220 - endostatin was transformed into dh5a, and expressed mouse endostatin protein successfully. this study is the basis of the endostatin gene - engineering production
本文成功克隆鼠內皮抑素基因,構建了內皮抑素基因的原核表達載體pbv220一endostatin ,該載體為非融合性表達載體,將pbv22o一endostatin成功轉化到大腸桿菌表達菌dh5a中,並誘導表達了鼠內皮抑素蛋白,為基因工程方法生產內皮抑素奠定一定基礎。Using ex vivo gene transfer technique, exogenous gene was introduced to the liver graft during cold preservation and express locally in the graft. the effect of inhibition of rejection and inducing liver graft tolerance was observed. through this study, the possibility of achieving graft tolerance by gene transfer without routine immunosuppressive drugs was explored
我們採用adeasy腺病毒載體系統,自行構建攜帶huctla4 - ig的重組腺病毒,通過先體外后體內( exvivo )基因轉移技術,于供肝冷保存時,將該治療基因導入大鼠移植肝,使其于移植肝局部表達,觀察其抑制排斥反應,誘導大鼠移植肝免疫耐受的作用。In this paper, three human dna fragments were coinjected into mice in order to study the influence of uncoding large dna fragment on hsa or hfen1 gene ' s integration efficiency, to explore methods to produce high expression of human serum album animals, to find out how to establish multi - transgenic animals in a way of saving time and money, to provide experience and reference to others. it mainly contains the following aspects : firstly, according to the sequence of human serum album gene cdna in genebank, one primer was designed
本實驗以小鼠為研究對象,採用兩種基因? hsacdna 、 hfen1和一種非編碼大片段dna進行共注射,旨在探索非編碼大片段dna對hsa和hfen1基因整合的影響及建立提高hsa基因整合效率的轉基因動物的方法,同時,探索多基因轉基因動物建立的策略,以便尋求一種既節省時間,而又簡單經濟的轉基因方法,為以後的研究提供一點的探索性經驗和參考。The study of ischemic mechanisms has been vastly abetted by the use of transgenic and knockout mouse mutants
轉基因和被擊昏的老鼠突變體的使用極大地支持了對缺血機械的研究。Influences of chinese herbal medicine compound on insulin and frame muscle glucose transport 4 gene expression of type 2 diabet in rat
糖脈平膠囊對2型糖尿病大鼠胰島素及骨骼肌葡萄糖轉運體4基因表達的影響分享友人