酵母片 的英文怎麼說

中文拼音 [jiàomǔpiān]
酵母片 英文
yeast
  • 酵 : 動詞（發酵） ferment; leaven
  • 母 : Ⅰ名詞1 （母親） mother 2 （泛指女性長輩） one s elderly female relatives 3 （配套的兩件東西里的凹...
  • 片 : 片構詞成分。
  • 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
  1. Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control

    選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
  2. Transgenic plants were comfirmed by molecular determination including pcr, southern blot and rt - pcr. 4. functional analysis and iron content of transgenic plant crude proteins of transgenic plant were extracted from leaves, and then used to test its antibiotic activities

    4 .轉基因植株抗菌能力加強，鐵含量得到提高提取轉基因植株葉片粗蛋白，以大腸桿菌dhsq和酵母菌ahiog為試驗靶標，側定了轉基因植株粗蛋白的抑菌活性。
  3. Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5 - dctp or cy3 - dctp into the reaction during reverse transcription of rna

    採用cartesian基因晶元列印儀，將純化的cdna片段列印在corning玻片表面，製作了酵母細胞基因表達譜晶元。
  4. It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation

    通過電轉化作用該基因片段被成功地整合至酵母cs115的染色體上，經過甲醇誘導之後，該基因得到了分泌表達。
  5. 6, we used gcn5 and rpd3 genes as probes to detect the homologous sequences in drosophila melanogaster by fluorescence in situ hybridization ( fish ). this work has provided useful information for the localization and cloning of related histone acetyltransferase and histone deacetylase genes in drosophila melanogaster

    6 ，利用己獲得的酵母gcns和rpd3基因為探針，對果蠅多線染色體進行原位雜交實驗，試圖找出與gcns和rpd3基因同源的基因片段。為今後克隆和分離果蠅中與乙酞化和去乙酚化相關的基因奠定基礎。
  6. The main research contents and results are as follows : ( 1 ) disc papers and oxford cups were used to ascertain bacteriostatic effect of hongqu. using 70 % ethanol and water as solvent, the extraction inhibited bacillus subtilis, bacillus cereus and staphylococcus aureus and had no effect on e. coli, salmonella. typhi, yeasts and algae

    主要研究內容和結果如下： （ 1 ）利用濾紙片法和管碟法確定了紅曲的抑菌譜：紅曲70乙醇和水溶液浸提物對蠟樣芽孢桿菌、枯草芽孢桿菌等革蘭氏陽性菌具有較強的抑制作用，對革蘭氏陰性菌、黴菌和酵母菌沒有抑制效果。
  7. Direct smear of urine from a patient with candidiasis of the kidney showing c. albicans in mycelial or tissue phase with blastoconidia budding from the pseudohyphae

    第二張圖片：是用患有腎念珠菌病的患者的小便做的直接塗片，顯示有菌絲的白假絲酵母菌。以出芽方式增殖，在組織內可見芽生孢子和假菌絲。
  8. In summary, we applied rd - pcr technique to the isolation of cdna fragments, which, compared with the conventional cloning procedure, is more speedy and higher throughput. the rd - pcr cdna fragments were constructed into a s. cerevisiae est ( expressed sequence tags ) library, which lay the foundation of cdna probe preparation and preparation of cdna microarray

    綜上所述，本研究採用rd ? pcr技術，探索了利用該技術快速分離大量cdna片段的具體過程，並建立了酵母cdna文庫及酵母cdna探針文庫，為dna晶元的制備打好基礎。
  9. In order to express the recombinant peptide of both gp52 and pp150 oterminal peptides from human cytomegalovirus ( hcmv ), which seem to show good antigenicity. recombinant dna technology was used to construct recombinant plasmid, which was transformed into the pichia pastoris to express the interesting peptide

    為了表達人巨細胞病毒（ humancytomegalovirus ， hcmv ）中抗原性較強的兩段蛋白片段? gp52c末端和pp150c末端的嵌合肽，用基因工程技術構建適于酵母表達系統的重組表達質粒。
  10. In our work, different hbv pres gene fragments were amplified by pcr, cloned into pgbkt ? vector in yeast two - hybrid system 3, and then transformed ah 109 yeast cells. the results showed that these pres expression products were nontoxic to yeast cells, but all self - activated the yeast two - hybrid system

    本研究將hbv前s區不同片斷進行擴增，克隆入酵母雙雜交系統3中的pgbkt7載體，轉化酵母細胞ah109 ，經檢測，證實了hbv前s區不同片斷對酵母細胞無毒性作用，但是都存在自激活作用。
  11. The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase. main results of this research are as follow : 1, xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser. 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418. they all grew quickly on both md plates and mm plates, which proved their phenotypes of methanol utilization were mut +

    主要實驗結查如下：西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句） a ，回收dna ，用genepulser電擊轉化畢赤酵母，塗布md平板，又用含不同濃度g418的ypd平板進行抗性篩選，得到98個可檢測到植酸酶活力的陽性轉化子，它們在md 、 mm平板上均表現快速生長，說明其甲醇利用表型是mut干。
  12. Baba fareed has also the distinction of writing the first piece of urdu poetry

    酵母酒蛋糕fareed並且有寫urdu詩歌第一片斷的分別。
  13. 11 interacting candidates proved to be the positives after isolating the mixed plasmids from yeast and recotransforming each purified ad / library plasmid and pgbd - nifa into s. cerevisiae pj69 - 4a respectively. the genes of the 11 positives had been sequenced and 4 different gene fragments were obtained

    分別對其中的ad / library質粒進行分離純化，將每個純化質粒再次與誘餌質粒共轉化酵母pj69 - 4a進行驗證，得到11個陽性克隆，測序結果顯示其中有4個不同的基因片段，分別命名為s4 、 s35 、 s64和s65 。
  14. The high at content of the native gene is very difficult to express, we designed a new he gene by reverse the codon used for e. coli and yeast, then, the he gene was cloned into the expression vector pqe - 60

    本研究嘗試了其保護性抗原基因在大腸桿菌及酵母中的表達。天然bont a的hc片段基因中at含量高達76 ，其通過同義密碼子置換人工合成hc段基因，將at含量降到57 。
  15. Multi - copies insertion transformants were screened on g418 plates. the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay. the n - carbamoylase activity of recombinant was 2. 26 and 2. 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169

    將hyucdna片段連接到真核表達載體ppic3 . 5k上，經bgl酶切線性化，通過peg法轉化導入畢赤酵母gs115感受態細胞，利用g418抗性篩選得到12個插入多拷貝目的基因的轉化子。
  16. The moose a cow and her calf had become drunk over the weekend by eating fermented apples they found outside the home in sibbhult, southern sweden, said anna karlsson, who works there

    工作人員安娜卡爾森表示，這兩頭馴鹿其實是一頭成年母鹿和它的幼崽，它們于上周末在養老院里發現了一些已經發酵的蘋果，在飽餐一頓后便迅速沉浸到了一片醉意之中。
  17. Methods : 1. expression and purification of recombinant human ctla4 extracellular domain : a 400bp dna fragment of ctla4 extracellular domain was obtained from the pctla4 / ig plasmid with genetical technique. then, this dna fragment was inserted into ppic9 plasmid to construct the single copy plasmid of yeast expression system ( ppic9 - ctla4125 ). furthermore, the multi - copy plasmid ( ppic9k - ctla4125 ) was constructed

    重組人ctla4胞外區蛋白在酵母gs115中的表達和純化：首先通過基因操作技術，從pctla4八g質粒中擴增400hp的ctla4胞外區片段；將ctla4胞外區片段插人ppicg質粒中獲取單拷貝酵母表達質粒ppicg ctla4125並進一步構建多拷貝酵母表達質粒ppicgk （ 。
  18. Clones were isolated and gene fragments were sequenced. subsequent gene sequence database were also establishen to manage the identified gene information. the purified cdna fragments were used as probes for the gene chips and arrayed upon microscopic slides by cartesian microarrayer

    本研究利用rd ? pcr （ restrictiondisplay - pcr ， rd ? pcr ）技術，從酵母細胞中分離出大量cdna片段，克隆和構建了cdna片段文庫，進一步將單基因片段純化出來，建立了相應的數據庫對所有信息進行管理。
  19. The 1. 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e. coli strains ( ff4169 ). otsa gene is in charge of trehalose - 6 - phosphate synthesis in e. coli. in growth curve experiment, the transformants that carried the 1. 488kb dna fragment grew well in minimum medium, which contains 0. 5mol / l nacl, while control strains could n ' t endure it

    提取釀酒酵母的總dna ，以此為模板，採用pcr的方法從釀酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段，通過xba和sma雙酶切，與同樣經過xba和sma雙酶切的puc118載體質粒連接，轉入大腸桿菌dh5中，通過藍白斑篩選重組子。
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