酶多肽合成 的英文怎麼說
中文拼音 [duōtàigěchéng]
酶多肽合成
英文
enzymatic peptide synthesis-
In this paper, the relationship between cyanide - resistant respiration and expression of alternative oxidase ( aox ) using polyantibodies prepared by synthetic polypeptide in mung bean seedling, as well as the effects of vernalization on cyanide - resistant respiration and expression of aox in winter wheat were studied respectively. in the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic s helix of aox. were synthesized by solid - phase method
第一部分,我們按照交替氧化酶( alternativeoxidase , aox )位於線粒體內膜外側親水區s螺旋的氨基酸序列,用固相法合成由12個氨基酸殘基組成的多肽,將此多肽與-牛胰凝乳蛋白酶原a相連,用作半抗原,免疫家兔制備人工合成12肽的抗體。The gene of amoa in ammonia - oxidizing encodes the active - site polypeptide of ammonia monooxygenase which catalyzes the oxidation of ammonia to hydroxylamine. we designed a pair of primers special for the amoa gene by comparing the known amoa gene sequences and used pcr to amplify the amoa gene fragments
Amoa基因是編碼氨單加氧酶活性多肽位點基因,我們通過引物篩選合成了對氨氧化細菌amoa基因特異結合的引物序列,利用pcr技術對活性污泥中的amoa基因片段進行特異擴增,得到的dna片段大約為490bp 。Objective : polypeptide : n - acetylgalactosaminyl transferase is the initiation enzyme catalyzing the linkage of o - glucan chain. recent study shows that o - glucosylation is closely related to molecular recognition, tumor formation, development and metastasis, as well as embryonic development. due to the initial study on function of o - glucosylation in china, this thesis aims to obtain stably expressed pp - galnac - t2 gene clones for further study
目的多肽: n乙酰氨基半乳糖轉移酶是合成o糖鏈的起始酶,而目前的研究認為, o -糖基化與分子及細胞識別、腫瘤的發生發展和轉移以及胚胎發育等功能密切相關。Construction and expression of tandem multi - copy gene edcoding ca ( 1 - 8 ) me ( 1 - 10 ) hybrid fused acidic peptide to improve antimicrobial peptide ' s expression, a novel mass - production method is proposed. it is based on the neutralization of the positive charges of antimicrobial peptide by fusing to an acidic peptide to avoid the lethal effect of the expressed peptide on the host cells
為提高抗菌肽的表達,設計在抗菌肽基因的n端融合一段編碼酸性短肽的片段作為對抗菌肽前體的模擬,以減輕表達產物對宿主的毒性;同時通過含有酶切位點的接頭將該融合肽基因以同向串連的方式連接成多拷貝基因。Twenty - six induced proteins were analyzed by matrix assisted laser desorption / ionization time - of - flight mass spectrometry ( maldi - tof / ms ) and identified by database searching. among them, 20 ones were assigned and their functions were known, including salt stress proteins, or enzymes are related to salt stress, y - butyrobetaine and iipopolysaccharide synthesis, metabolism pathway and signal transduction
應用maldi - tof ms對26個鹽激誘導蛋白進行分析,將獲得的肽指紋圖譜經數據庫檢索,初步確定了20個誘導蛋白的功能,其中包括與鹽脅迫、 -丁酰甜菜堿、脂多糖合成、代謝途徑和信號傳導等有關的酶。While there are some difference in amino acid with other group ( except tw 95 ), the amino acid in 65 - 75 of hn of ndv group are conserved, while he in 81 is conserved. based on epitope prediction, five pairs primers are designed with enzyme sites. five segments of f1, f2, f3, f4 and hn1 coding epitope was amplified and cloned to prokaryotic expression vector, respectively
在表位預測的基礎上,將臨近的表位合併,設計引物並引入酶切位點,以fa 、 fb 、 hna為模板擴增含抗原表位的多肽片段f1 、 f2 、 f3 、 f4 、 hn1 ,克隆到原核表達載體ppro ~ ( ex ) ht上,成功地構建了五個表達載體。In yeast, the synthesis of trehalose is catalyzed by trehalose - 6 - phosphate synthase / phosphatase complex, which is encoded by four genes : tpsl, tps2, tps3 and tsl1
酵母細胞中的海藻糖由tps1 、 tps2 , tps3和tsl1四個基因負責合成,其編碼的多肽組成一個海藻糖合酶復合體。分享友人